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Summary of Investigations Conducted at CEFAS into the Effects of Oyster Matrix on HPLC and MBA PSP Results


<p>This discrepancy in results could not be explained through any method performance issues associated with the HPLC analysis and so (further to some previously documented research findings) it was assumed that matrix components within the oysters may be influencing toxicity estimations. Given the importance of demonstrating the equivalence of the two methodologies prior to any potential implementation of HPLC methods into official control programmes, the potential causes of these findings were further investigated.</p>

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<p>Background: Previous validation work on the AOAC 2005.06 HPLC method highlighted significant differences in method performance between the High Performance Liquid Chromatography with Fluorescence Detection (HPLC-FLD) and Mouse Bioassay (MBA) official control methods for the quantitation of PSP toxins (PSTs) in oysters. Results showed that the total PSP toxicities determined using HPLC are on average 200% and 280% higher than the MBA toxicity results for Pacific oysters and native oysters respectively, as compared with a better correlation between the two methods for mussels and cockles (mean HPLC/MBA = 101% and 133% respectively). </p>

Centre for Environment, Fisheries and Aquaculture Sciences (CEFAS)
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