DEVELOPMENT AND VALIDATION OF NOVEL DIAGNOSTIC TOOLS FOR RAPID AND EARLY DETECTION OF EMERGING INFECTION FROM ASFV VARIANTS

The overall goal of this project is to develop novel diagnostic tests and provide uniqueresources for validation of African swine fever (ASF) diagnostic tools. Objectives of our research efforts include:Collect field clinical samples from pigs naturally infected with ASFV in commercial swine farms in Vietnam and the Philippines.Collect field clinical samples from ASF-free pigs in local farms in the United States.Transfer the collected clinical samples from Vietnam and the Philippines to the United States.Develop, optimize and validate cELISA system using p30 protein as the capture antigen and mAb3007 as the competitive antibody.Express CD2v protein with Baculovirus Expression System and generate anti-CD2v mAbs.Develop, optimize and validate cELISA system using CD2v protein as the capture antigen and anti-CD2v mAb as the competitive antibody.Develop, optimize and validate a reliable multiplex real-time PCR assay for ASFV variants testing.