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DEVELOPMENT OF METHODS AND STRATEGIES TO IMPROVE THE MICROBIOLOGICAL SAFETY OF AQUACULTURE PRODUCT

Objective

The objectives are: a) develop rapid nucleic acid extraction techniques for molecular detection of hepatitis A virus and Norwalk virus in molluscan shellfish by RT-PCR-based analyses and develop inclusive primer sets for RT-PCR detection of Norwalk-like viruses, b) develop cell culturebased assays for the enumeration of hepatitis A and human caliciviruses, and c) evaluate the uptake, distribution and elimination of enteric viruses and vibrios from shellfish maintained under simulated natural conditions.

Investigators
Richards, Gary
Institution
USDA - Agricultural Research Service
Start date
2001
End date
2006
Project number
1935-42000-045-00D
Accession number
404298