DEVELOPMENT OF A NANO-BIOSENSOR TO DETECT GLUCOSE FOR DIABETICS USING RECOMBINANT MANGANESE PEROXIDASE FROM CORN GRAIN

The overall dual goal of this project is to strengthen A-State's research emphasis and improve collaboration in the area of chemistry and biosciences, as well as to enhance participation of minorities and women in STEM disciplines and careers.Hypothesis: Our hypothesis is that an electrochemical sensor based on PPMP can be developed that is 5-fold more sensitive than the HRP-based sensor and that can resist fouling for 50% longer.Objectives to prove this hypothesis:Generate a two-layered electrochemical biosensor membrane applying GOx with a novel electrocatalytic enzyme, PPMP, via crosslinking.Enhance the sensitivity of the biosensor using nanoparticles.Apply an antifouling polymer during biosensor modification to enable real world measurements.