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To find specific mutations in bacteriophage Å"ÃœX174 that render the bacteriophage non-infectious and that will revert to plaque-forming ability only when mutation occurs by specific mechanisms: 1. Base substitution at a G:C base pair or 2. Frameshift caused by deletion of one or two nucleotides. An additional objective is to determine the feasibility of using Å"ÃœX174 to detect 3. The deletion of an extended sequence. Phage harboring these mutations will be used to construct a transgenic mouse model for measuring mutations in vivo.
To find specific mutations in bacteriophage 0X174 that render the bacteriophage non-infectious and that will revert to plaque-forming ability only when mutation occurs by specific mechanisms: 1) base substitution at a G:C base pair or 2) frameshift caused by deletion of one or two nucleotides. An additional objective is to determine the feasibility of using 0X174 to detect 3) the deletion of an extended sequence. Phage harboring these mutations will be used to construct a transgenic mouse model for measuring mutations in vivo.