The novel protocol to measure HGT from algae to non-photosynthetic organisms illustrated in Figure 1, with Eq. (1) for the HGT rates [3], will be used to quantitively measure the rates of HGT from GE cyanobacteria to soil-environmental bacteria (Objective 1). It will also be used to characterize the mechanisms whether the HGT is through a direct cell-to-cell interaction or by an indirect cell-to-liquid environment (eDNA)-to-cell process (Objective 2). In addition, the stability of the transgenes and the survivability of GE cyanobacteria in comparison with their related wild-type organisms will be assessed (Objective 3).
GE CYANOBACTERIA BIOSAFETY ASSESSMENT RESEARCH
Objective
Investigators
Lee, J.
Institution
OLD DOMINION UNIVERSITY RESEARCH FOUNDATION
Start date
2023
End date
2026
Funding Source
Project number
VA.W-2023-02441
Accession number
1031327