The objective of this cooperative research project is to evaluate the extent of dissemination of Salmonella from gastrointestinal lumen to regional lymph nodes within 3 hours of inoculation.
Nine pigs will be fed probiotics. Rectal swabs will be taken every 3 to 4 days after inoculation. On day 17, all 12 pigs will be inoculated (intranasal) with Salmonella typhimurium 3 hours prior to necropsy. Space allocations will be maintained as per ACUC guidelines. Histopathology and microbiological evaluations will be performed after necropsy.