HOST-PATHOGEN INTERACTION DURING CRYPTOSPORIDIOSIS A MODEL FOR DISEASE PATHOGENESIS AND DISCOVERY OF EFFECTIVE THERAPEUTICS

AIM 1:Assess the impact of age andIFN-L3on theinherentimmune signature induced by IEC-DC crosstalk afterex vivoCP-exposure.AIM 1A. Assess transcriptomicand metabolicprofiles,cytokine secretionand cellular phenotypeinduced by CP-exposed IEC-MoDCcrosstalk.AIM 1B. Evaluate IEC-MoDC induced T cell activation following CP-exposure.AIM 2: Assess the impact of soluble factors, in an age-dependent manner, and IFl-L3,on the immune signature induced by IEC-DC crosstalk afterex vivoCP-exposureAIM 2A. Assess the impact of soluble mediators onthetranscriptomicand metabolicprofiles, cytokine secretion, and phenotypeof CP-exposed IEC-MoDC.AIM 2B.Evaluatethe impact ofIEC-MoDC-exposed to soluble mediators on induced T cell activation following CP-exposure.AIM 3:Assess the impact ofin vivoCP infection on the immune signature induced by ex vivo IEC-DC crosstalk during concurrent IFN-l3 exposure.AIM 3A. Assess metabolic,transcriptomic profile,cytokine secretion, and phenotypeinduced by CP-exposed IEC-MoDC.AIM 3B. Evaluate IEC-MoDC induced T cell activation following CP-exposure.