1. Develop new or refine current methods for filtration and concentration of P. ramorum propagules in spiked nursery water samples. 2. Test antibodies reactive against P. ramorum with water samples containing infectious propagules of P. ramorum, focusing on detection in irrigation and surface water samples from the National Ornamental Research Site -Dominican University of California (NORS-DUC) mock nursery site. 3. Refine immunoassay diagnostic protocols for nursery water samples.