Microbial Genetics of Non-0157:H7 Shiga-Like Toxin Producing Escherichia Coli Insulated From Humans and Foods

Objective

<p>
<ol>
<li>Obtain E. coli isolates from clinical, food-related outbreaks and veterinary diagnostics samples.</li>
<li>Map the epidemiological profiles of the isolates for specific genetic markers attributable to the origin of isolates and their phenotypic diversity.</li>
<li>Determine the antimicrobial-resistance profiles and potential mechanisms of drug resistance among EHECs of various serogroups.</li>
<li>Identify the antimicrobial-resistance and virulence gene determinants in the bacterial isolates that contribute to their pathogenicity.</li>
<li>Examine the role of plasmids, if any, in mitigating the transfer of drug resistance.</li>
<li>Compare the cytoxicities of selected EHEC strains to cultured RAW264.7 macrophage cells.</li>
<li>Evaluate the expression of Shiga-like toxin using in vitro enzyme assays.</li></ol></p>

More information

Responsible Division: Microbiology

Investigators

Nayak, Rajesh

Institution

DHHS/FDA - National Center for Toxicological Research

Funding Source

Nat'l. Center For Toxicological Research

Project number

E0735701