Studies on Mechanism of Fluoroquinolones Resistant Salmonella spp. Isolated from Animal Feeds (Poultry), Animal Production Environment and the Development of Molecular Methods for Screening the Drug Resistance Genes

Objective

<p>1) To isolate, identify and characterize nalidixic acid and fluoroquinolone-resistant Salmonella spp. from chicken farms (animal feed, feces, manure,litters, and animals) by biochemical and polymerase chain reaction. 2) To determine minimum inhibitory concentration for environmental isolates, development of molecular techniques and its comparison with clinical strains. 3) To determine drug-resistance mechanisms in the environmental isolates and their characterization by molecular techniques. 4) To determine influence of seasons and the frequency of isolation of fluoroquinolone-resistant Salmonella spp.</p>

More information

FY 2000 Accomplishments: 1) Developed multiplex PCR method for the rapid detection of multidrug-resistant Salmonella typhimurium. 2) Developed mismatch amplification mutation assy for the detection of ciprofloxacin-resistant Salmonella spp. 3) Isolated several nalidixic acid (antibiotic)-resistant E. coli from chicken and turkey farms. 4) Characterized fluoroquinolones-resistant Campylobacter spp. From chicken and turkey farms by pulse-field gel electrophoresis.

FY2001 Plans: 1) Isolation and molecular characterization of fluoroquinolone-resistant Salmonella spp. And E. coli from chicken and turkey litters. 2) Determine the influence of climatic conditions on the occurrence of quinolone-resistant Salmonella spp. And E. coli.

Investigators

Khan, Ashraf

Institution

DHHS/FDA - National Center for Toxicological Research

Start date

2000

End date

2001

Funding Source

Nat'l. Center For Toxicological Research

Project number

E0704801