<p>1) To isolate, identify and characterize nalidixic acid and fluoroquinolone-resistant Salmonella spp. from chicken farms (animal feed, feces, manure,litters, and animals) by biochemical and polymerase chain reaction. 2) To determine minimum inhibitory concentration for environmental isolates, development of molecular techniques and its comparison with clinical strains. 3) To determine drug-resistance mechanisms in the environmental isolates and their characterization by molecular techniques. 4) To determine influence of seasons and the frequency of isolation of fluoroquinolone-resistant Salmonella spp.</p>
FY 2000 Accomplishments: 1) Developed multiplex PCR method for the rapid detection of multidrug-resistant Salmonella typhimurium. 2) Developed mismatch amplification mutation assy for the detection of ciprofloxacin-resistant Salmonella spp. 3) Isolated several nalidixic acid (antibiotic)-resistant E. coli from chicken and turkey farms. 4) Characterized fluoroquinolones-resistant Campylobacter spp. From chicken and turkey farms by pulse-field gel electrophoresis.
FY2001 Plans: 1) Isolation and molecular characterization of fluoroquinolone-resistant Salmonella spp. And E. coli from chicken and turkey litters. 2) Determine the influence of climatic conditions on the occurrence of quinolone-resistant Salmonella spp. And E. coli.