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Use of Protein Profiles to Characterise the Concentration Effects Curve for Mixtures of Estrogenic Compounds


This research project aims to investigate the effects of low concentrations of combinations of estrogenic chemicals.

<p>This proposal will utilise a novel approach to conduct a mechanistically-based investigation into the effects of low concentrations of combinations of estrogenic chemicals, determining the profile of expressed proteins in human breast tumour cell lines.

<p>Protein profiling of cell lines varying in estrogen receptor status will be undertaken using SELDI-TOF mass spectrometry.

<p>Those proteins changing significantly will be identified by conventional proteomic techniques and assessed for biological relevance.

<p>This could form the basis of a new way of assessing responsiveness.

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The possibility that mixtures of dietary chemicals, such as estrogenic compounds, act synergistically is a cause for concern.

<p>Such chemicals act directly, via estrogen receptors, or indirectly to modulate expression of estrogen-responsive genes.

<p>If successful this work will reveal how the proteome of the cell responds to mixtures of estrogenic compounds and to fully characterise the concentration-effect curve.

<p>Find more about this project and other FSA food safety-related projects at the <a href="; target="_blank">Food Standards Agency Research webpage</a>.

Imperial College - London
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