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Validation of Testing Methods for the Detection and Quantification of Escherichia Coli 0157:H7, Salmonella SPP


The objective of this study is to evaluate TMECC and EPA pathogen detection methods in order to accurately determine the presence of populations of E. coli O157:H7, Salmonella, non-pathogenic E. coli, and fecal coliforms in ‘point of sale’ composts collected from commercial operations across the United States. Correlations between the presence of pathogens and several physico-chemical parameters (moisture, water activity, electrical conductivity, soluble carbon, pH, and carbon:nitrogen ratios) will be investigated.

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ARS will acquire the basic knowledge about the stresses that pathogenic E. coli or Salmonella strains undergo in compost and their prevalence in various types of compost. The carbon:nitrogen ratio will evaluated in commercial formulations. Based on these findings, compost formulations using specific C:N ratios will then be formulated to determine survival and inhibition of E. coli and Salmonella.

USDA - Agricultural Research Service
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