Research Publications from Peer-Reviewed Journals

Wed, 10/16/2019 - 03:29
ABSTRACT To further our understanding of one-carbon metabolism in the protozoan parasite Leishmania, we conducted genomic screens to study how the parasite responded to sinefungin (SNF) selection. SNF is a structural analogue of S-adenosylmethionine (AdoMet), a key methyl group donor to a number of biomolecules. One screen consisted of sequencing SNF-resistant mutants generated by stepwise...
Wed, 10/16/2019 - 03:28
This study reports the use of reverse transcription – loop-mediated isothermal amplification (RT–LAMP) to detect Listeria monocytogenes in meat. The assay was designed to target the iap gene of L. monocytogenes, to which four primers, recognizing six distinct iap sites, were designed. We optimized the RT–LAMP conditions and established the following optimal systems: 60 min, 63 °C, 2.0 mmol/L...
Wed, 10/16/2019 - 03:28
Author(s): Armand Van Deun, Tom Decroo, Sabira Tahseen, Arnaud Trébucq, Valérie Schwoebel, Nimer Ortuno-Gutierrez, Bouke C. de Jong, Hans L. Rieder, Alberto Piubello, Chen-Yuan ChiangAbstractThe 2018 World Health Organization (WHO) treatment guidelines for multidrug-/rifampicin-resistant tuberculosis (MDR/RR-TB) give preference to all-oral long regimens for 18-20 months. The document strongly...
Wed, 10/16/2019 - 03:25
Author(s): Yujing Zhang, Yu Ma, Ningjuan Liang, Yuehui Liang, Chan Lu, Fang XiaoAbstractHexavalent chromium [Cr(VI)] is a common heavy metal pollutant widely used in various industrial fields. It is well known that mitochondria are the most vulnerable targets of heavy metals, but the key molecule/event that directly mediated mitochondrial dysfunction after Cr(VI) exposure is still unclear. The...
Wed, 10/16/2019 - 03:25
Author(s): Huiyu Wang, Shanjun Song, Mingwu Shao, Yan Gao, Chen Yang, Ya Li, Weihua Wang, Yajuan He, Penghui LiAbstractFreshness protection packages and preservative films are widely used food-contact plastic made of polyethylene. Diode array detector (DAD), charged aerosol detector (CAD) and evaporative light-scattering detector (ELSD) were evaluated for determination of 6 bisphenols (bisphenol...
Wed, 10/16/2019 - 03:22
Chilled mutton is favored in the Chinese market for its rich nutrition and delicious taste. However, storage temperature can be higher than 4°C to reduce energy and transportation costs, which makes chilled mutton vulnerable to decomposition by microorganisms causing a high attrition rate of chilled mutton. The physical property method is a general widely applicable method to evaluate meat...
Wed, 10/16/2019 - 03:21
The capsaicin in hot peppers is an important biological active substance that is widely used in food and medicine. Our current study shows that capsaicin derivatives have good antioxidant and antibacterial activities, and therefore, the present study of capsaicin derivatives with good activity provides a good foundation for future applications in natural food additives and medicine. Abstract The...
Wed, 10/16/2019 - 03:21
Promotion of healthier lifestyles has led to an increase in consumption of fresh produce. Such foodstuffs may expose consumers to increased risk of foodborne disease, as often they are not subjected to processing steps to ensure effective removal or inactivation of pathogenic microorganisms before consumption. Consequently, reports of ready‐to‐eat fruit and vegetable related disease outbreak...
Wed, 10/16/2019 - 03:21
To combat food scarcity as well as to ensure nutritional food supply for sustainable living of increasing population, microalgae are considered as innovative sources for adequate nutrition. Currently, the dried biomass, various carotenoids, phycocyanin, phycoerythrin, omega fatty acids, and enzymes are being used as food additives, food coloring agents, and food supplements. Apart from...
Wed, 10/16/2019 - 03:21
Nonthermal processing methods are often preferred over conventional food processing methods to ensure nutritional quality. Nonthermal plasma (NTP) is a new field of nonthermal processing technology and seeing increased interest for application in food preservation. In food applications of NTP, liquid interactions are the most prevalent. The NTP reactivity and product storability are altered...
Wed, 10/16/2019 - 03:21
The current research focuses on anaerobic respiration of arsenic and other toxic metals by purple nonsulfur bacteria (PNSB). Among the optimization assays performed were carbon utilization, cross metal resistance, and metal respiration, along with a comparison of each assay in photoheterotrophic and chemoheterotrophic growth. The bacteria were identified by the classification of 16S ribosomal RNA...
Wed, 10/16/2019 - 03:20
Cobamides are a family of cofactors that are widely‐utilized by diverse organisms, and there are over a dozen variants, including vitamin B12. V. cholerae imports a range of cobamides, but it cannot utilize a variant called pseudocobalamin and instead remodels it through a distinct pathway. Abstract Cobamides are a group of compounds including vitamin B12 that can vary at the lower base position...
Wed, 10/16/2019 - 03:18
Little is known about the presence and effects of polychlorinated biphenyls (PCBs) and organochlorine pesticides (OCPs) in Puerto Rico’s waters. Four coastal aquatic systems were investigated using low-density polyethylene passive sampling for PCBs and OCPs in water and its overlying air. The highest total freely dissolved and gaseous concentrations of PCBs were found in Guánica Bay, with 4000 pg...
Wed, 10/16/2019 - 03:18
Functional foods provide nutritional and health benefits, yet they could be contaminated with residues like pesticides and polychlorobiphenyls. These residues affect the safety, quality, and consequently the commercial value of functional foods. Therefore, the validity and efficiency of residue determination methods constitute a major analytical concern. Reduction of matrix effect (ME) has always...
Wed, 10/16/2019 - 03:17
Although many proteins possess a distinct folded structure lying at a minimum in a funneled free energy landscape, thermal energy causes any protein to continuously access lowly populated excited states. The existence of excited states is an integral part of biological function. Although transitions into the excited states may lead...
Wed, 10/16/2019 - 03:17
Mycotoxin removers include enzymes and adsorbents that may be used in animal feeds to eliminate the toxic effects of mycotoxins. This study aimed to determine the removability of two different types of mycotoxin removers, adsorbents and enzyme degradation reagents (EDRs), in the simulated gastrointestinal conditions of pigs and poultry. Seven commercial mycotoxin removers, including five EDRs and...
Wed, 10/16/2019 - 03:17
Resistance to antimicrobial agents of pathogenic bacteria has become a major problem in routine medical practices. Carbapenem resistance has long been increasing. The production of carbapenem- hydrolysing β-la...
Wed, 10/16/2019 - 03:17
Brucellosis is one of the most severe widespread zoonoses caused by the Gram-negative bacterium Brucella species. The diagnosis and clinical assessment of human brucellosis are very important for the management o...
Tue, 10/15/2019 - 03:34
by Juan Delgado-SanMartin, Beate Ehrhardt, Marcin Paczkowski, Sean Hackett, Andrew Smith, Wajahat Waraich, James Klatzow, Adeala Zabair, Anna Chabokdast, Leonardo Rubio-Navarro, Amar Rahi, Zena Wilson Introduction In oncological drug development, animal studies continue to play a central role in which the volume of subcutaneous tumours is monitored to assess the efficacy of new drugs. The tumour...
Tue, 10/15/2019 - 03:34
by Thi T. Hang Pham, Thuy X. Le, Dong T. Nguyen, Chau M. Luu, Bac D. Truong, Phu D. Tran, Mehlika Toy, Selen Bozkurt, Samuel So Background and aim Vietnam’s burden of liver cancer is largely due to its high prevalence of chronic hepatitis B virus (HBV) infection. This study aimed to examine healthcare workers’ (HCWs) knowledge, attitude and practices regarding HBV prevention and management....
Tue, 10/15/2019 - 03:33
Escherichia coli O157:H7 is a foodborne pathogen, implicated in various multi-state outbreaks. It encodes Shiga toxin on a prophage, and Shiga toxin production is linked to phage induction. An E. coli strain, designated 0.1229, was identified that amplified Stx2a production when co-cultured with E. coli O157:H7 strain PA2. Growth of PA2 in 0.1229 cell-free supernatants had a similar effect, even...
Tue, 10/15/2019 - 03:33
Author(s): Erfaneh Amoueyan, Sajjad Ahmad, Joseph N.S. Eisenberg, Daniel GerrityAbstractThis study describes the results of a dynamic quantitative microbial risk assessment (QMRA) for norovirus (NoV) that was used to evaluate the relative significance of foodborne, person-to-person, and person-to-sewage-to-person transmission pathways. This last pathway was incorporated into simulated potable...
Tue, 10/15/2019 - 03:33
Author(s): Xiao Xiao, Rui Qi, Hui-Ju Han, Jian-Wei Liu, Xiang-Rong Qin, Li-Zhu Fang, Chuan-Min Zhou, Xiao-Qing Gong, Si-Cong Lei, Xue-Jie YuAbstractSnakes are popular as food and traditional medicine in China. However, information about parasitic and bacterial infections in snakes from China is scarce. We investigated the prevalence of selected zoonotic agents including Cryptosporidium,...
Tue, 10/15/2019 - 03:32
Author(s): Katesaraporn Nuankeaw, Boonyanoot Chaiyosang, Thanapat Suebrasri, Somdej Kanokmedhakul, Saisaamorn Lumyong, Sophon BoonlueAbstractBioactive compounds of endophytic fungus Trichoderma polyalthiae were extracted from culture broth media. The crude extracts showed strong antimicrobial activity against human pathogens. Biologically active compounds were isolated and purified by...
Tue, 10/15/2019 - 03:32
Author(s): Elumalai Preetham, Abdul Salam Rubeena, Baskaralingam Vaseeharan, Mukesh Kumar Chaurasia, Jesu Arockiaraj, Rolf Erik OlsenAbstractThe study is carried out to understand the antimicrobial and immunological response of a potential immune molecule lectin, MmLec isolated from haemolymph of Speckled shrimp, Metapenaeus monoceros. MmLec was purified using mannose coupled Sepharose CL-4B...
Tue, 10/15/2019 - 03:31
Author(s): J. Rosales, S.M. Pérez, M. Cabrera, M.J. Gázquez, J.P. Bolivar, J. de Brito, F. AgrelaAbstractVarious treatments of phosphogypsum samples extracted in Huelva, Spain, curing conditions and incorporation ratios were studied to evaluate the viability of being used as set regulator or as supplementary cementitious material to improve the mechanical behaviour of cement mortars.The physical-...
Tue, 10/15/2019 - 03:31
Author(s): Kezhen Yan, Hao Sun, Fengqian Gao, Dongdong Ge, Lingyun YouAbstractThe objective of the paper is to assess the feasibility of using solid waste incineration (MSWI) bottom ash (BA) as cement-stabilized macadam (CSM) aggregate. CSM was prepared by replacing natural limestone aggregate with MSWI BA in different proportions (0%, 5%, 10%, 20%, and 30%). The physical properties, chemical...
Tue, 10/15/2019 - 03:29
Author(s): E. Blázquez, C. Rodríguez, J. Ródenas, N. Navarro, R. Rosell, S. Pina-Pedrero, J.M. Campbell, M. Sibila, J. Segalés, J. Pujols, J. PoloAbstractLiquid porcine plasma is an animal origin raw material for the manufacturing process of spray-dried porcine plasma that is used in pig nutrition worldwide. In previous studies we found that the application of ultraviolet light C (UV-C) in liquid...
Tue, 10/15/2019 - 03:29
Author(s): Andreas Eisenreich, Benjamin Sachse, Rainer Gürtler, Birgit Dusemund, Oliver Lindtner, Bernd SchäferAbstractOpium alkaloids such as morphine and thebaine occur in the latex of Papaver somniferum varieties. Some varieties are used for both, pharmaceutical opium alkaloid generation and poppy seed production for food use. Poppy seeds can be contaminated with opium alkaloid-containing...
Tue, 10/15/2019 - 03:29
Author(s): A.-C. Geuthner, M. Koethe, M. Ludewig, S. Pott, G. Schares, P. Maksimov, A. Daugschies, B. BangouraAbstractTurkeys and chickens were orally infected with tissue cysts (one mouse brain) or oocysts (103, 105 or 106 oocysts) of three T. gondii strains of the clonal types II and III (ME49, CZ-Tiger, NED) to investigate the influence of the applied T. gondii strain and infective doses on...
Tue, 10/15/2019 - 03:28
Author(s): Xin Wang, Hao Yu, Hengtong Fang, Yun Zhao, Yongcheng Jin, Jinglin Shen, Changhai Zhou, Yongfeng Zhou, Yurong Fu, Junmei Wang, Jing ZhangAbstractMounting evidence has shown that zearalenone (ZEA) can have toxic effects on the intestinal epithelial cells (IECs) of mammals and humans, but the mechanism of ZEA-induced toxicity on IECs is unclear. The aim of this study was to reveal the...
Tue, 10/15/2019 - 03:23
Significance and Impact of the Study: Fermented chopped pepper is an important ingredient in traditional Chinese foods. As of yet, the bacterial community dynamics that take place during the fermentation of chopped pepper have not been described. In this study, we used high‐throughput sequencing to investigate the bacterial community, deepening our understanding of the microbial mechanisms...
Tue, 10/15/2019 - 03:23
Aspergillus flavus (A. flavus) is a pathogenic fungus that produces carcinogenic aflatoxins, posing a great threat to crops, animals and humans. Lysine acetylation is one of the most important reversible post‐translational modifications, and plays a vital regulatory role in various cellular processes. However, current information on the extent and function of lysine acetylation and aflatoxin...
Tue, 10/15/2019 - 03:22
Aims The object of this study was to identify potential health concerns of the Aeromons spp. isolated from marketed Pacific abalone (Haliotis discus hannai) with respect to their virulence and antimicrobial resistance patterns. Methods and Results We identified 29 strains of aeromonads consisting of 5 species; A. hydrophila (n=9), A. enteropelogenes (n=14), A. veronii (n=3), A. salmonicida (n=2)...
Tue, 10/15/2019 - 03:22
Aims This study was aimed at revealing the composition of microbiota in leaves, roots and rhizosphere soil of wheat plants that are resistant or susceptible to stripe rust, one of the most widely destructive leaf diseases in wheat production. Methods and Results A total of 36 wheat plants that showed resistant or susceptible reactions to stripe rust were sampled. Three compartments of each plant...
Tue, 10/15/2019 - 03:21
Dichlorvos (DDVP) is widely applied in the agricultural industry, and its residues are considered hazardous to the environment. Microbial bioremediation is an innovative technology with the potential to mitigate such pollution. Trichoderma atroviride strain T23, a filamentous fungus, is very efficient at degrading DDVP. Therefore, we used DDVP as a model organophosphate pesticide to study the...
Tue, 10/15/2019 - 03:21
Background In urban Australia, the burden of shigellosis is either in returning travelers from shigellosis-endemic regions or in men who have sex with men (MSM). Here, we combine genomic data with comprehensive epidemiological data on sexual exposure and travel to describe the spread of multidrug-resistant Shigella lineages. Methods A population-level study of all cultured Shigella isolates in...
Tue, 10/15/2019 - 03:21
Tuberculosis is the primary infectious disease killer worldwide, with a growing threat from multidrug-resistant cases. Unfortunately, classic growth-based phenotypic drug susceptibility testing (DST) remains difficult, costly, and time consuming, while current rapid molecular testing options are limited by the diversity of antimicrobial-resistant genotypes that can be detected at once. Next-...
Tue, 10/15/2019 - 03:20
Aflatoxins (AFs) as potent food contaminants are highly detrimental to human and animal health. The production of such biological toxins is influenced by environmental factors including pollutants, such as dioxins. Here, we report the biological feedback of an active AF-producer strain of A. flavus upon in vitro exposure to the most toxic congener of dioxins, the 2,3,7,8-tetrachlorinated dibenzo-...
Tue, 10/15/2019 - 03:20
Besides the major nutrients, phosphorus (P) is an essential mineral for both the host animal and the porcine gut microbiota. Different strategies including phytase supplementation and more recently lactic acid (LA) are used to enhance the P availability from cereals in pig diets; however, their impact on the gut microbiota has been rarely related to fecal shedding of opportunistic pathogens. The...
Tue, 10/15/2019 - 03:20
Both nosocomial pathogens, such as Streptococcus pneumoniae and Haemophilus influenzae and food-borne pathogens, such as Bacillus cereus and Clostridium perfringens are known to be detrimental to human and animal health. The effectiveness of currently used treatments for these pathogens becomes limited as resistant strains emerge. Therefore, new methods for eliminating bacterial pathogens must be...
Sun, 10/13/2019 - 03:29
Author(s): Xiaogang Du, Dong Li, Yunkun Li, Jiayu Wu, Anqi Huang, Guixian Bu, Fengyan Meng, Fanli Kong, Xiaohan Cao, Xingfa Han, Xiaofu Pan, Wei fan, Shiyong Yang, Xianyin ZengAbstractMammal Toll-like receptor 5 (TLR5) can directly recognize bacterial flagellin and therefore to initiate the inflammatory signaling cascades and trigger body immune system to clear the “non-self” substances. In...
Sun, 10/13/2019 - 03:29
Author(s): Shijuan Shan, Rongrong Liu, Hanxiao Feng, Yonghuan Zhang, Fumiao Zhang, Cui Lv, Guiwen YangAbstractp65 is an important subunit of the transcription factor NF-κB in the regulation of immune response. In the present study, the p65 cDNA was identified from common carp (Cyprinus carpio L.) (named Ccp65). Phylogenetic analysis revealed that Ccp65 located in the same clade as piscine p65 and...
Sun, 10/13/2019 - 03:29
Author(s): Yu-Lei Chen, Keke Han, Xin Huang, Zhuoxing Zhang, Xihe Wan, Qian RenAbstractThe caspase is an essential module in the Drosophila immune deficiency (IMD) pathway, which plays a crucial role in countering pathogen infection. In this study, a gene named PcCaspase-3C was found in Procambarus clarkia with a full-length of 4684 bp, including a 1572 bp opening reading frame, which encoded a...
Sun, 10/13/2019 - 03:29
Author(s): Zhi-qiang Du, Bo Li, Xiu-li Shen, Kai Wang, Jie Du, Xiao-dong Yu, Jian-jun YuanAbstractThe main advantage of antimicrobial peptides (AMPs) used as the effectors in the innate immunity system of invertebrates is that the high specificity is not indispensable. And they play important roles in the systemic defenses against microbial invasion. In this study, a new full-length cDNA of the...

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