Research Publications from Peer-Reviewed Journals

The Research Publications track research that is published across national and international peer-reviewed journals. The most recent articles are available ahead of print and searchable by Journal Title, Subject, USDA Grant Funding Agencies or FDA Grant Funding Agencies. The research publications are tracked across five subject areas: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, and Viruses.

Fri, 03/27/2020 - 03:40
by Mariana Cap, Celeste Cingolani, Carla Lires, Marina Mozgovoj, Trinidad Soteras, Adriana Sucari, Jimena Gentiluomo, Adriana Descalzo, Gabriela Grigioni, Marcelo Signorini, Celina Horak, Sergio Vaudagna, Gerardo Leotta The aim of this study was to assess the efficacy of lactic acid (LA), caprylic acid (CA), high- (HDI) and low- (LDI) dose gamma irradiation and LDI combined with LA or CA on the...
Fri, 03/27/2020 - 03:40
A large outbreak of extensively drug-resistant (XDR) Salmonella enterica serotype Typhi infections is ongoing in Pakistan, predominantly in Sindh Province. Here, we report the sequencing and characterization of five XDR Salmonella Typhi isolates from the Punjab province of Pakistan that are closely related to the outbreak strain and carry the same IncY plasmid.
Fri, 03/27/2020 - 03:40
This is an announcement for the genome sequence of a clinical isolate of Salmonella enterica subsp. arizonae isolated from the urine and prostate of a 6-year-old male Labrador retriever. This is one of the few reports of a Salmonella enterica subsp. arizonae isolate cultured from canine urine.
Fri, 03/27/2020 - 03:40
Salmonella enterica subsp. enterica isolates are the leading cause of foodborne illness worldwide. Here, we report the draft genomes of 26 Salmonella isolates of food and clinical origin, belonging to four serovars, associated with outbreaks from 1999 to 2006 in the south of Brazil.
Fri, 03/27/2020 - 03:40
Five strains of Salmonella enterica subsp. enterica serovar Infantis and two strains of S. enterica subsp. enterica serovar Kentucky isolated in 2017 from Ecuadorian layer poultry farms were sequenced using Illumina MiSeq technology. These isolates were collected on layer farms in central Ecuador, one of the most important areas of egg production in the country. The genome sequences of these...
Fri, 03/27/2020 - 03:40
We present here the complete genome sequences of three Salmonella enterica subsp. enterica serovar Muenchen strains, LG24, LG25, and LG26. All three strains were isolated from almond drupes grown in an orchard in San Joaquin County, California, in 2016. These genomic sequences are nonidentical and will contribute to our understanding of S. enterica genomics.
Fri, 03/27/2020 - 03:38
Author(s): Laura Haleva, William Lopes, Vanessa Barcellos, Augusto Schrank, Marilene H. Vainstein In nature, microorganisms often exhibit competitive behavior for nutrients and limited space, allowing them to alter the virulence determinants of pathogens. The human pathogenic yeast Cryptococcus neoformans can be found organized in biofilms, a complex community composed of an extracellular matrix...
Fri, 03/27/2020 - 03:37
Author(s): Yue Zhang, Yongkang Xie, Juming Tang, Shaojin Wang, Lu Wang, Guangfei Zhu, Xingyi Li, Yanhong Liu A 27.12 MHz thermostatic radio frequency (RF) system was employed to inactivate Cronobacter sakazakii in powdered infant formula milk. A proportional-integral-derivative controller achieved constant material temperature during the holding period. Hot air and similar dielectric material...
Fri, 03/27/2020 - 03:36
Author(s): Carla Barría, Randall S. Singer, Irene Bueno, Erika Estrada, Dácil Rivera, Soledad Ulloa, Jorge Fernandez, Fernando O. Mardones, Andrea I. Moreno-Switt Artisanal cheese from southern Chile is made primarily by rural families who raise dairy cows and produce cheese as a way to add value to their milk. The most common cheese produced is chanco, a semi-hard cheese that is typically sold...
Fri, 03/27/2020 - 03:36
Author(s): Mingxing Tian, Yi Yin, Zhengmin Lian, Zichen Li, Meiying Song, Hai Hu, Xiang Guan, Chan Ding, Shaohui Wang, Tao Li, Jingjing Qi, Shengqing Yu Brucella is a facultative intracellular bacterium, dividing into smooth- and rough-type Brucella. Smooth-type Brucella can dissociate into rough mutants with cytotoxicity for macrophages during infection, which is critical for Brucella egress and...
Fri, 03/27/2020 - 03:32
An analytical method is applied to simultaneously determine bisphenol A, 4-octylphenol, 4-tert-octylphenol, 4-nonylphenol and tert-nonylphenol in fish muscle. The method consisted of a cleanup on molecularly imprinted polymers cartridges (MIPs) followed by liquid chromatography coupled to a tandem mass spectrometer. The method is rapid, specific, reliable and very sensitive, allowing for...
Fri, 03/27/2020 - 03:32
Total mercury levels and methylmercury levels were investigated for various grain parts (whole rice, rice husk, brown rice, polished rice, and bran) of 507 rice samples from 15 main rice-producing areas of China. The average total mercury contents in brown rice samples and polished rice samples were 4.2 and 3.3 μg/kg, respectively, the percentages exceeding the national standard limit were 0.59%...
Fri, 03/27/2020 - 03:31
Atmospheric cold plasma (ACP) is an emerging technology in the food industry with a huge antimicrobial potential to improve safety and extend the shelf life of food products. Dielectric barrier discharge (DBD) is a popular approach for generating ACP. Thanks to the numerous advantages of DBD ACP, it is proving to be successful in a number of applications, including microbial decontamination of...
Fri, 03/27/2020 - 03:31
SUMMARY Since the first introduction of African swine fever (ASF) into the European wild boar population in 1957, the question of virus survival in carcasses of animals that succumbed to the disease has been discussed. The causative African swine fever virus (ASFV) is known to be very stable in the environment. Thus, carcasses of infected wild boar could play a major role as ASFV reservoir and...
Fri, 03/27/2020 - 03:30
Gaseous chlorine dioxide (ClO2) is a promising sanitizer for frozen products because of its efficacy under nonthermal and waterless conditions. Our pilot‐scale treatment of blueberries demonstrated that a significant reduction of 1.5 log CFU/g can be achieved against coliforms after ClO2 exposure. Our findings demonstrate a cost‐effective procedure that could be adapted to commercial processing....
Fri, 03/27/2020 - 03:30
The combination of nisin, EDTA, and sulfite at a low dose of 0.625% exhibited higher antimicrobial activity than did a high dose of sulfite (1.25%) for shrimp preservation. Based on aerobic bacteria counts, psychrotrophic bacteria, and TVB‐N, shrimp treatment with combination of nisin, EDTA, and low‐dose sulfite were still acceptable within 10 days of storage. Abstract This study aims to...
Fri, 03/27/2020 - 03:24
ABSTRACT Antimicrobial resistance (AMR) has emerged as one of the most pressing threats to public health. AMR evolution occurs in the clinic but also in the environment, where antibiotics and heavy metals can select and co-select for AMR. While the selective potential of both antibiotics and metals is increasingly well-characterized, experimental studies exploring their combined effects on AMR...
Fri, 03/27/2020 - 03:24
ABSTRACT Little is known about the regulatory mechanisms that ensure the survival of the food-borne bacterial pathogen Listeria monocytogenes in the telluric environment and on roots. Earlier studies have suggested a regulatory overlap between the Agr cell–cell communication system and the general stress response regulator σB. Here, we investigated the contribution of these two systems to root...
Fri, 03/27/2020 - 03:24
Drug-induced kidney injury is a serious safety issue in drug development. In this study, we evaluated the usefulness of adult zebrafish as a small in vivo system for detecting drug-induced kidney injury. We first investigated the effects of typical nephrotoxicants, gentamicin and doxorubicin, on adult zebrafish. We found that gentamicin induced renal tubular necrosis with increased lysosome and...
Fri, 03/27/2020 - 03:23
Bacterial persisters are rare phenotypic variants that are temporarily tolerant to high concentrations of antibiotics. We have previously discovered that stationary-phase-cell subpopulations exhibiting high redox activities were less capable of producing proteins and resuming growth upon their dilution into fresh media. The redox activities of these cells were maintained by endogenous protein and...
Fri, 03/27/2020 - 03:23
Campylobacter spp. are one of the most important food-borne pathogens, which are quite susceptible to environmental or technological stressors compared to other zoonotic bacteria. This might be due to the lack of many stress response mechanisms described in other bacteria. Nevertheless, Campylobacter is able to survive in the environment and food products. Although some aspects of the heat stress...
Fri, 03/27/2020 - 03:23
We read with interest the study by Michaela J Day and colleagues,1 which announced that the correlation between foodborne extended-spectrum β-lactamase (ESBL)-producing Escherichia coli and human-derived isolates was low, and that the human-to-human, oral–faecal route contributed more to the transmission of human-derived ESBL-producing E coli than the food chain.
Fri, 03/27/2020 - 03:23
A survey on 120 cereal samples (barley, maize, rice and wheat) from Algerian markets has been carried out to evaluate the presence of 15 mycotoxins (ochratoxin A, deoxynivalenol, fumonisin B1 and B2, T-2 and HT-2 toxins, zearalenone, fusarenon X, citrinin, sterigmatocystin, enniatins A, A1, B and B1, and beauvericin). With this purpose, a QuEChERS-based extraction and ultra-high performance...
Fri, 03/27/2020 - 03:23
In countries with intensive pig husbandry in stables, the prevalence of livestock-associated (LA) methicillin-resistant Staphylococcus aureus (MRSA) on such farms has remained high in the last few years or has also further increased. Simple measures to reduce the LA-MRSA among pigs have not yet been successfully implemented. Earlier publications showed a decontamination of LA-MRSA was only...
Thu, 03/26/2020 - 03:35
Recent developments in preservation technologies allow for the delivery of food with nutritional value and superior taste. Of special interest are low-acid, shelf-stable foods in which the complete control or inactivation of bacterial endospores is the crucial step to ensure consumer safety. Relevant preservation methods can be classified into physicochemical or physical hurdles, and the latter...
Thu, 03/26/2020 - 03:35
In general, foodborne diseases present themselves with gastrointestinal symptoms caused by bacterial, viral, and parasitic pathogens well established to be foodborne. These pathogens are also associated with extraintestinal clinical manifestations. Recent studies have suggested that Escherichia coli and Klebsiella pneumoniae, which both cause common extraintestinal infections such as urinary...
Thu, 03/26/2020 - 03:35
Recent advances in food packaging materials largely rely on nanotechnology structuring. Owing to several unique properties of nanostructures that are lacking in their bulk forms, the incorporation of nanostructures into packaging materials has greatly improved the performance and enriched the functionalities of these materials. This review focuses on the functions and applications of widely...
Thu, 03/26/2020 - 03:35
Functional foods is a very popular term in the social and scientific media; consequently, food producers have invested resources in the development of processed foods that may provide added functional benefits to consumers’ well-being. Because of intrinsic regulation and end-of-use purposes in different countries, worldwide meanings and definitions of this term are still unclear. Hence, here we...
Thu, 03/26/2020 - 03:35
Fusarium verticillioides is the primary fungus that causes Fusarium ear rot (FER) of maize. Infection results in reduced grain yield and quality due to moulding and the contamination of grain with toxic compounds namely fumonisins. Resistance to fungal infection and fumonisin accumulation in maize and maize grain is governed at different levels. In this study, the structural, physico-chemical and...
Thu, 03/26/2020 - 03:24
ABSTRACT Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacteriaceae have become widespread in hospitals and the environment. Here, we describe a blaKPC-2-carrying plasmid called pCRE3-KPC, which was recovered from a clinical multidrug-resistant Citrobacter braakii CRE3 strain in China. The complete nucleotide sequence of pCRE3-KPC was determined by combining MiSeq and MinION...
Thu, 03/26/2020 - 03:24
The Revogene Carba C assay (formerly GenePOC Carba assay) is a multiplex nucleic acid-based in vitro diagnostic test intended for the detection of carbapenemase-producing Enterobacterales (CPE) from cultured colonies. This assay was evaluated directly on colonies of 118 well-characterized Enterobacterales with reduced susceptibility to carbapenems and on 49 multidrug-resistant (MDR) Pseudomonas...
Thu, 03/26/2020 - 03:24
Aztreonam-avibactam is a combination antimicrobial agent with activity against carbapenemase-producing Enterobacteriaceae (CPE) with metallo-β-lactamases (MβLs). Although aztreonam-avibactam is not yet approved by the U.S. Food and Drug Administration (FDA), clinicians can administer this combination by using two FDA-approved drugs: aztreonam and ceftazidime-avibactam. This combination...
Thu, 03/26/2020 - 03:23
Whole-genome sequencing has enhanced surveillance and facilitated detailed monitoring of the transmission of Shigella species in England. We undertook an epidemiological and phylogenetic analysis of isolates from all cases of shigellosis referred to Public Health England between 2015 and 2018 to explore recent strain characteristics and the transmission dynamics of Shigella species. Of the 4,950...
Thu, 03/26/2020 - 03:23
Rapid identification from positive blood cultures is standard of care (SOC) in many clinical microbiology laboratories. The GenMark Dx ePlex Blood Culture Identification Gram-Positive (BCID-GP) Panel is a multiplex nucleic acid amplification assay based on competitive DNA hybridization and electrochemical detection using eSensor technology. This multicenter study compared the investigational-use-...
Thu, 03/26/2020 - 03:23
Plasmid-mediated colistin resistance (PMCR) is a global public health concern, given its ease of transmissibility. The purpose of this study was to evaluate two methods for the detection of PMCR from bacterial colonies: (i) the NG-Test MCR-1 lateral flow immunoassay (LFA; NG Biotech, Guipry, France) and (ii) the EDTA-colistin broth disk elution (EDTA-CBDE) screening test method. These methods...
Thu, 03/26/2020 - 03:23
U.S. gonorrhea rates are rising, and antibiotic-resistant Neisseria gonorrhoeae (AR-Ng) is an urgent public health threat. Since implementation of nucleic acid amplification tests for N. gonorrhoeae identification, the capacity for culturing N. gonorrhoeae in the United States has declined, along with the ability to perform culture-based antimicrobial susceptibility testing (AST). Yet AST is...
Thu, 03/26/2020 - 03:23
Candida auris is a multidrug-resistant yeast which has emerged in health care facilities worldwide; however, little is known about identification methods, patient colonization, environmental survival, spread, and drug resistance. Colonization on both biotic (patients) and abiotic (health care objects) surfaces, along with travel, appear to be the major factors for the spread of this pathogen...
Thu, 03/26/2020 - 03:23
A rapid and accurate method to identify the species and antibiotic resistance of Candida spp. in blood is vital to increase the survival rates of patients with bloodstream infections. However, the extremely low levels of Candida spp. in blood make rapid diagnosis by standard blood culture difficult. In this study, we constructed a direct blood culturing method (i.e., the M1 method) by a rapid...
Thu, 03/26/2020 - 03:23
Bartonella spp. are etiological agents of life-threatening zoonotic diseases in dogs worldwide. Due to the poor sensitivity of immunofluorescent-antibody assays (IFAs), a reliable serodiagnostic test for canine bartonelloses is of clinical importance. The utility of Western blotting (WB) for the serodiagnosis of canine bartonelloses has not been critically investigated. The objective of this...
Thu, 03/26/2020 - 03:23
Rapid diagnostic tests (RDTs) for bloodstream infections (BSIs) decrease the time to organism identification and resistance detection. RDTs are associated with early deescalation of therapy for Gram-positive BSIs. However, it is less clear how RDTs influence antibiotic management for Gram-negative BSIs and whether RDT results are acted on during off-hours. We performed a single-center,...
Thu, 03/26/2020 - 03:22
Author(s): T. Mazaheri, C. Ripolles-Avila, A.S. Hascoët, J.J. Rodríguez-Jerez Biofilms increase the probability of microorganism survival and potentiate subsequent food product cross-contamination. The search for alternatives capable of eliminating the matrix produced by these communities, and accessing to the cells that made them up, is of real interest. This study aimed to evaluate the...
Thu, 03/26/2020 - 03:21
Author(s): M.Á. Martínez, J. Blanco, J. Rovira, V. Kumar, J.L. Domingo, M. Schuhmacher This study was aimed at comparing the toxicity effects on cell viability and the obesogenic activity of Bisphenol A (BPA) and its analogues, Bisphenol S (BPS) and Bisphenol F (BPF), by in vitro assays with a preadipocytic 3T3-L1 cell line. To compare the toxic potential and select the concentrations of each...
Thu, 03/26/2020 - 03:20
Seafood can be a source of contaminants, which may raise health concerns. The aim of this study was to analyse the levels of inorganic contaminants in commercially available seafood products and assess consumer exposure. Commercially available samples were collected from 2015–2018 and analysed as composite samples for mercury, lead, arsenic, and cadmium, using accredited methods. Levels of...
Thu, 03/26/2020 - 03:18
The study was to evaluate the food safety knowledge and hygiene practices among food handlers. A total of 42 food handlers in 13 basic schools under the School Feeding Scheme, Soweto, South Africa were recruited for the study using purposive and convenience sampling methods for the respondents and institutions, respectively. A piloted self‐administered questionnaire was used. All the respondents...
Thu, 03/26/2020 - 03:16
We previously reported that immunostaining for γ‐H2AX, a biomarker of DNA damage, in the rat urinary bladder is useful for early detection of bladder carcinogens in 28‐day toxicity studies. Here, we aimed to examine the dose dependency of γ‐H2AX formation in the urinary bladder of rats. Male F344 rats (aged 6 weeks) were orally administered N‐butyl‐N‐(4‐hydroxybutyl)nitrosamine (BBN; 0%, 0.0001...
Thu, 03/26/2020 - 03:16
Assessment of reproductive toxicity is one of the important safety considerations in drug development. Thus, in the present research, the naïve Bayes (NB)‐classifier method was applied to develop binary classification models. Six important molecular descriptors for reproductive toxicity were selected by the genetic algorithm. Then, 110 classification models were developed using six molecular...
Thu, 03/26/2020 - 03:16
The aim of the study was to assess the antibacterial properties of bioactive compounds released during the fermentation of goat’s milk by selected bacterial strains that are part of the kefir grain microflora. The material used in the experiments was kefir grain microflora (Lactobacillus kefiranofaciens subsp. kefirgranum DSM 10550, Lactobacillus kefiri PCM 2501, Lactobacillus parakefiri DSM...
Thu, 03/26/2020 - 03:16
Free and protein-bound Maillard reaction products (acrylamide, 5-hydroxymethylfurfural, α-dicarbonyl compounds, N-ε-fructosyllysine, N-ε-carboxymethyllysine and N-ε-carboxyethyllysine) and the reaction precursors (sugars and amino acids) were investigated in bread crust-like systems prepared with different cereal flours. Selected cereals were; whole and refined wheat, whole einkorn, whole rye,...
Thu, 03/26/2020 - 03:16
This work reports on the method optimization and application for quantitative analysis of non-steroidal anti-inflammatory drugs and anti-epileptic drug in soil and sediment samples. The analytes were extracted by ultrasonic extraction followed by solid phase extraction and quantified using liquid chromatographic coupled with photodiode array. The sensitivity of the method was determined based on...
Thu, 03/26/2020 - 03:16
Reliable and sensitive detection of Staphylococcal enterotoxin B (SEB) is important to prevent potential human health risk from foods contaminated by SEB. Here, a triple-channel assay was developed for ultrasensitive detection of SEB. Based on the optical characteristics of tetramers composed of gold nanoparticles (AuNPs) and upconversion nanoparticles (UCNPs), the SEB amounts in food products...

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