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Bacterial pathogens

Mon, 07/01/2019 - 00:00
Escherichia coli O157:H7 and Salmonella choleraesuis are two important foodborne pathogens that cause illnesses and even deaths. Rapid and convenient methods, such as immunochromatographic assays (ICAs), are useful for detecting these two pathogens. Herein, we developed a highly sensitive ICA for the simultaneous quantitative analysis of these two foodborne pathogens. Silver...
Tue, 06/18/2019 - 01:12
Available online 16 June 2019 Author(s): Gabriele Meroni, Valentina Zamarian, Cristina Prussiani, Valerio Bronzo, Cristina Lecchi, Piera Anna Martino, Fabrizio CecilianiAbstractStaphylococcus aureus biofilm-related infections are of clinical concern due to the capability of bacterial colonies to adapt to a hostile environment. The present study investigated the capability of the acute phase...
Mon, 06/17/2019 - 13:13
Abstract Aims The control of Listeria monocytogenes biofilm formation using lactocin AL705 bacteriocin at sub‐MICs through an anti‐quorum sensing strategy, was preliminarily investigated. Methods and Results The screening for biofilm formation of different Listeria species at 10 °C allowed selecting L. monocytogenes FBUNT for its use as biofilm producer. Minimum inhibitory concentration (MIC)...
Mon, 06/17/2019 - 11:00
Methylmercury (MeHg) is a potent bioaccumulative neurotoxin that is produced by certain anaerobic bacteria and archaea. Mercury (Hg) methylation has been linked to the gene pair hgcAB, which encodes a membrane-associated corrinoid protein and a ferredoxin. Although microbial Hg methylation has been characterized in vivo, the cellular biochemistry and the specific roles of the gene products HgcA...
Mon, 06/17/2019 - 11:00
Ceftiofur, a third-generation cephalosporin antimicrobial, was used in Canadian hatcheries for many years to prevent early mortality in chicks, leading to a high prevalence of cephalosporin resistance in Escherichia coli in chickens. Preventive use of ceftiofur in hatcheries ceased in 2014. We examined the effect of ceftiofur cessation (n = 40 flocks with ceftiofur and n = 28 flocks without...
Mon, 06/17/2019 - 11:00
The effectiveness of antibiotics has been challenged by the increasing frequency of antimicrobial resistance (AMR), which has emerged as a major threat to global health. Despite its negative impact on the development of AMR, there are few effective strategies for reducing AMR in food-producing animals. Using whole-genome sequencing and comparative genomics of 36 multidrug-resistant (MDR)...
Mon, 06/17/2019 - 01:15
Available online 16 June 2019 Author(s): Paolo Bonilauri, Maria Silvia Grisenti, Paolo Daminelli, Giuseppe Merialdi, Mattia Ramini, Lia Bardasi, Roberta Taddei, Elena Cosciani-Cunico, Elena Dalzini, Maria Angela Frustoli, Federica Giacometti, Silvia Piva, Andrea SerrainoAbstractThis study involved ten enterprises producing Italian salami, 20 different samples of fermented sausages underwent...
Mon, 06/17/2019 - 01:15
Available online 15 June 2019 Author(s): Ifeoluwa Adekoya, Adewale Obadina, Momodu Olorunfemi, Olamide Akande, Sofie Landschoot, Sarah De Saeger, Patrick NjobehAbstractIn Africa, fermented foods and beverages play significant roles in contributing to food security. Endotoxins are ubiquitous heat stable lipopolysaccharide (LPS) complexes situated in the outer cell membranes of Gram-negative...
Sun, 06/16/2019 - 01:12
Available online 14 June 2019 Author(s): Yu-Lin Lee, Min-Chi Lu, Pei-Lan Shao, Po-Liang Lu, Yen-Hsu Chen, Shu-Hsing Cheng, Wen-Chien Ko, Chi-Ying Lin, Ting-Shu Wu, Muh-Yong Yen, Lih-Shinn Wang, Chang-Pan Liu, Wen-Sen Lee, Zhi-Yuan Shi, Yao-Shen Chen, Fu-Der Wang, Shu-Hui Tseng, Chao-Nan Lin, Yu-Hui Chen, Wang-Huei ShengAbstractWe performed multicentre surveillance of the antimicrobial...
Sat, 06/15/2019 - 01:07
Available online 13 June 2019 Author(s): Qin Liu, Xiaoxuan Jin, Xiao Feng, Hongshun Yang, Caili FuAbstractIn the present study, the synergistic disinfection efficacy of low concentration electrolyzed water (LcEW) (free available chlorine, 4 mg/L) combined with brief heat enhancement was evaluated and the bactericidal mechanism was investigated by atomic force microscopy (AFM). The inactivation...
Sat, 06/15/2019 - 01:07
Available online 12 June 2019 Author(s): Hang Qi, Yen-Con HungAbstractActivated persulfate could be an alternative sanitizer for fresh produce decontamination. This study is the first in reporting the potential of activated persulfate in reducing Escherichia coli O157:H7 and Listeria monocytogenes from romaine lettuce surface. Ferrous sulfate and sodium hydroxide were used as the activators. Both...
Sat, 06/15/2019 - 01:07
Available online 14 June 2019 Author(s): Sandy Thao, Maria T. Brandl, Michelle Qiu CarterAbstractBacterial persistence is a form of phenotypic heterogeneity in which a subpopulation, persisters, has high tolerance to antibiotics and other stresses. Persisters of enteric pathogens may represent the subpopulations capable of surviving in harsh environments and causing human infections. Here we...
Sat, 06/15/2019 - 01:07
Available online 13 June 2019 Author(s): Yong Ma, Sujuan Ding, Yanquan Fei, Gang Liu, Hongmei Jang, Jun FangAbstractThe main foodborne pathogens are Norovirus, Enteric parasites, bacteria and fungi. Foodborne diseases can lead to high morbidity and mortality and result in huge economic losses. Some foodborne diseases are associated with antibiotic resistance, which is a significant problem...
Fri, 06/14/2019 - 03:00
Journal of Food Protection, Volume 82, Issue 7, Page 1104-1109, July 2019.
Fri, 06/14/2019 - 01:12
Available online 13 June 2019 Author(s): Khashayar Shahin, Majid Bouzari, Ran Wang, Mahsa YazdiAbstractShigella spp. can be isolated from various food sources and is responsible for many outbreaks and sporadic cases of foodborne diseases worldwide. Although Shigella species are known as one of the major foodborne pathogens, a few studies have characterized the prevalence and molecular basis of...
Fri, 06/14/2019 - 01:12
Available online 13 June 2019 Author(s): Romina Zanabria, Manon Racicot, Alexandre Leroux, Liu Xucen, Mathieu Cormier, Cécile Ferrouillet, Julie Arsenault, Anna Mackay, Mansel Griffiths, Rick Holley, Tom Gill, Sylvain Charlebois, Jeffrey Farber, Aamir Fazil, Sylvain QuessyAbstractDecreasing the health burden caused by foodborne pathogens is challenging and it depends on the identification of the...
Fri, 06/14/2019 - 01:12
Available online 12 June 2019 Author(s): Putu Eka Sudaryatma, Hirohisa Mekata, Meiko Kubo, Mawar Subangkit, Yoshitaka Goto, Tamaki OkabayashiAbstractBovine respiratory disease complex is a major disease affecting the global cattle industry. Multiple infections by viruses and bacteria increase disease severity. Previously, we reported that bovine respiratory syncytial virus (BRSV) infection...
Thu, 06/13/2019 - 20:00
Shigella has evolved as a result of acquiring extragenetic material through horizontal gene exchange. These aid in the rapid emergence of bacterial inter-strain diversity in virulence factors and serotype variant...
Thu, 06/13/2019 - 10:28
Alginate‐based edible coating acts as a carrier enabling the sustained release of thyme oil to the surface of the fresh‐cut apples, inhibiting the growth of Listeria monocytogenes, Salmonella Typhimurium, Staphylococcus aureus and Escherichia coli O157:H7 inoculated onto fresh‐cut apples. Summary In this study, the inhibition of an alginate‐based edible coating (EC) containing thyme oil (0.05...
Thu, 06/13/2019 - 01:17
Available online 12 June 2019 Author(s): Xuan Zhang, Yan Dong Niu, Yuchen Nan, Kim Stanford, Rick Holley, Tim McAllister, Claudia Narváez-BravoAbstractThe purpose of this study was to determine the effectiveness of a commercial Salmonella bacteriophage mixture (SalmoFresh™ 6-phage strains) and to compare its effectiveness with a chlorinated water treatment to reduce Salmonella on produce and...
Thu, 06/13/2019 - 01:17
Available online 11 June 2019 Author(s): Caterina Agrimonti, Jason C. White, Stefano Tonetti, Nelson MarmiroliAbstractCellulosic pads, amended with emulsions containing essential oils of thyme and oregano, exhibited antimicrobial activity against the psychrophilic microbiota of minced beef. In addition, the pads were active against specific meat bacterial species (Pseudomonas putida, Pseudomonas...
Thu, 06/13/2019 - 01:17
Publication date: Available online 11 June 2019Source: Author(s): Virginia M. Guitián, Carolina Ibarguren, Cecilia M. Soria, Paula Hovanyecz, Claudia Banchio, Carina M. AudisioAbstractBacteriocins synthesised by lactic acid bacteria are natural alternatives to control Listeria monocytogenes, a foodborne pathogen. Additionally, antimicrobial biopolymer coatings have significant benefits in food...
Thu, 06/13/2019 - 01:17
Available online 12 June 2019 Author(s): Ornella M Ontañon, Silvina Ghio, Rubén Marrero Díaz de Villegas, Mercedes M Garrido, Paola M Talia, Csaba Fehér, Eleonora CamposAbstractGlycoside hydrolase family 8 (GH8) includes endoglucanases, lichenases, chitosanases and xylanases, which are essential for polysaccharides breakdown. In this work, we studied a thermally stable GH8 from the cellulose...
Thu, 06/13/2019 - 01:17
Available online 12 June 2019 Author(s): Lin Lin, Xinlei Wang, Haiying CuiAbstractPulsed magnetic fields (PMF) as a non-thermal sterilization has broad application prospects, but at this stage its bactericidal effect is not satisfactory. The essential oils have been proved with good antimicrobial effects. However, they will cover the original flavor of the food products at high dosages. In order...
Thu, 06/13/2019 - 01:17
Available online 11 June 2019 Author(s): Qingqing Xu, Jianping Jiang, Zhenhan Zhu, Teng Xu, Zi-Ke Sheng, Meiping Ye, Xiaogang Xu, Minggui WangAbstractKlebsiella pneumoniae is one of the common pathogens causing urinary tract infections (UTIs). Nitrofurantoin (NIT), with high therapeutic concentrations in urine, is recommended as first-line drug for both empiric treatment and chemoprophylaxis of...
Wed, 06/12/2019 - 01:13
Available online 11 June 2019 Author(s): Yoon-Jae Jeong, Jae-Won HaAbstractThis study was conducted to evaluate the antimicrobial effect of the combined treatment of UV-A radiation (UVA) and acetic acid (AA) against Escherichia coli O157:H7, Salmonella Typhimurium, and Listeria monocytogenes on spinach leaves and to investigate the effect of this combined treatment on product quality. Spinach...
Wed, 06/12/2019 - 01:13
Available online 10 June 2019 Author(s): Isabella de Medeiros Barbosa, Erika Tayse da Cruz Almeida, Lúcio Roberto Cançado Castellano, Evandro Leite de SouzaAbstractThis study evaluated whether the pre-exposure (24, 48 and 72 h) to sublethal conditions caused by acetic acid (AA), lactic acid (LA), sodium chloride (NaCl) or potassium chloride (KCl) could induce increased cross-tolerance to the...
Wed, 06/12/2019 - 01:13
Publication date: September 2019Source: LWT, Volume 112Author(s): Viridiana Cortés-Rodríguez, Lidia Dorantes-Alvarez, Humberto Hernández-Sánchez, Norma Paniagua-Castro, Gerardo Aparicio-Ozores, Edgar Oliver López-Villegas, María de Jesús Perea-FloresAbstractThe aim of this study was to investigate the effect of mixtures of sodium cinnamate, coumarate, caffeate and ferulate against L....
Wed, 06/12/2019 - 01:13
Publication date: September 2019Source: LWT, Volume 112Author(s): Dong-Gyun Yim, Jin-Kyu Seo, Hyeon-Woong Yum, Md Ashrafuzzaman Zahid, Jun-Young Park, Rashida Parvin, Jonghyun Go, Sang-Keun Jin, Ok-Kyung Koo, Han-Sul YangAbstractThe effects of C. sappan L. extract on oxidative stability, microbiological quality, and color attributes in sausages were examined. Pork sausages were prepared for four...
Wed, 06/12/2019 - 00:00
The present study investigated whether delaying the first feeding of colostrum affected ileum and colon mucosa-associated microbiota in calves. Twenty-seven male Holstein calves were randomly assigned to 1 of 3 groups, fed colostrum at 45 min, 6 h, and 12 h after birth, respectively. Ileum and colon mucosa were collected at 51 h after birth, and their associated microbial profiles were assessed...
Wed, 06/12/2019 - 00:00
Escherichia coli has been frequently reported as a major foodborne bacterium contaminating raw milk or pasteurized milk. Therefore, the aim of this study was to explore a quantitative real-time PCR (qPCR) technique combined with sodium dodecyl sulfate (SDS) and propidium monoazide (PMA) to detect culturable E. coli in milk. An internal amplification control was also added into this reaction...
Wed, 06/12/2019 - 00:00
The process of fermentation contributes to the organoleptic properties, preservation, and nutritional benefits of food. Fermented food may interfere with pathogen infections through a variety of mechanisms, including competitive exclusion or improving intestinal barrier integrity. In this study, the effect of milk fermented with Lactococcus lactis ssp. cremoris JFR1 on Salmonella invasion of...
Tue, 06/11/2019 - 20:00
The aim of this study was to identify and characterize LA-MRSA in a very recent collection of staphylococci isolated from bovine quarter milk samples. All milk samples (n=14,924) sent to the MBFG in March 2017 were included into this study. The samples originated from 3,887 cows with 3,367 samples from 2,280 animals being positive for bacteria, prototheca and/or yeast. The second most common...
Tue, 06/11/2019 - 04:54
Abstract This study aimed to functionalize the stainless steel surface with silver and to evaluate its antimicrobial capacity. For this purpose, stainless steel coupons coated or not with silver films were exposed to the presence of Escherichia coli and Staphylococcus aureus. The results of bacterial adhesion on the silver functionalized coupons showed lower counts of adhered S. aureus cells...
Tue, 06/11/2019 - 04:54
Abstract This study aimed to functionalize the stainless steel surface with silver and to evaluate its antimicrobial capacity. For this purpose, stainless steel coupons coated or not with silver films were exposed to the presence of Escherichia coli and Staphylococcus aureus. The results of bacterial adhesion on the silver functionalized coupons showed lower counts of adhered S. aureus cells...
Tue, 06/11/2019 - 04:54
Abstract Concerns over the safety of nitrite and its role in the formation of nitrosamines have aroused considerable interest in developing alternative antimicrobial agents to replace nitrite. Plant‐derived antimicrobial agents could serve against food spoilage and pathogens. For the first time in this study, three semiempirical kinetic models, that is, exponential, homographic, and n‐order...
Tue, 06/11/2019 - 04:54
Abstract Concerns over the safety of nitrite and its role in the formation of nitrosamines have aroused considerable interest in developing alternative antimicrobial agents to replace nitrite. Plant‐derived antimicrobial agents could serve against food spoilage and pathogens. For the first time in this study, three semiempirical kinetic models, that is, exponential, homographic, and n‐order...
Tue, 06/11/2019 - 01:14
Publication date: August 2019 , Volume 122Author(s): Hyo-Eun Kim, Jin-Jae Lee, Min-Jung Lee, Bong-Soo KimAbstractChicken meat is one of the most widely consumed meats worldwide. The microbiota on the whole body of chicken is a potential source of foodborne pathogens that can be transmitted to humans during the preparation of raw meat. However, to date, there have been no studies comparing the...
Mon, 06/10/2019 - 15:04
Abstract Aim Shiga toxin‐producing Escherichia coli (STEC) cause bloody diarrhoea, kidney failure and occasionally death. However, identifying the source of infection caused by STEC other than serogroup O157 is hampered by availability of sensitive methods for detecting these pathogens. In this study we developed novel tools for detecting E. coli O55 that is potentially associated with human...
Mon, 06/10/2019 - 12:53
Salmonella must rapidly adapt to various niches in the host during infection. Relevant virulence factors must be appropriately induced, and systems that are detrimental in a particular environment must be turned off. Salmonella infects intestinal epithelial cells using a type 3 secretion system (T3SS) encoded on Salmonella pathogenicity island 1 (SPI1). The system is controlled by three AraC-like...
Mon, 06/10/2019 - 12:53
Salmonella virulence requires the initial invasion of host cells followed by the modulation of the intracellular environment for survival and replication. In an effort to characterize the role of small RNAs in Salmonella pathogenesis, we inadvertently identified a 5 kD protein named YshB that is involved in the intracellular survival of Salmonella. We show here that yshB expression is upregulated...
Mon, 06/10/2019 - 01:13
Available online 8 June 2019 Author(s): Abdelrahim Hussein Abdelazeem Hassan, José Francisco Bergua, Eden morales-Narváez, Arben MekoçiAbstractConsidering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on...
Sun, 06/09/2019 - 20:00
Internalins are surface proteins that are utilized by Listeria monocytogenes to facilitate its invasion into human intestinal epithelial cells. The expression of a full-length InlA is one of essential virulence f...
Sun, 06/09/2019 - 14:39
Abstract Livestock meat and offal contribute significantly to human nutrition as sources of high‐quality protein and micronutrients. Livestock products are increasingly in demand, particularly in low‐ and middle‐income settings where economies are growing and meat is increasingly seen as an affordable and desirable food item. Demand is also driving intensification of livestock keeping and...
Sun, 06/09/2019 - 01:14
Available online 7 June 2019 Author(s): Jiajing Guo, Zhipeng Gao, Gaoyang Li, Fuhua Fu, Zengenni Liang, Hui Zhu, Yang ShanAbstractListeria monocytogenes (L. monocytogenes) is an important foodborne pathogen which may cause listeriosis and poses potential risks to human health. The aim of this study was to estimate the efficacy of essential oil from Citrus Changshan-huyou Y.B. Chang (Huyou) on...
Sun, 06/09/2019 - 01:14
Available online 8 June 2019 Author(s): Jing Xie, Yen-Con HungAbstractTiO2 nanoparticle (NP)-embedded cellulose acetate (CA) films were prepared using solution casting method and found that it can inactivate Escherichia coli O157: H7 under UV-A light illumination. This study investigated the microbial inactivation efficacy of this film under several practical application scenarios: (1) direct...
Sat, 06/08/2019 - 01:13
Available online 7 June 2019 Author(s): Clarissa Rech Peixoto, Paulo Armendaris, Andrius Grassi, Fabiani Andréa Walker Hengles, Eduardo Cesar TondoAbstractRestaurants used to prepare tenderloin beef medallions using different frying parameters in order to satisfy their customers. However, some of those cooking methods are not in accordance with food safety recommendations, because they do not...
Sat, 06/08/2019 - 01:13
Publication date: Available online 7 June 2019Source: Author(s): Molly S. Moritz, William H. Tepp, Heather N'te Inzalaco, Eric A. Johnson, Sabine PellettAbstractBotulinum neurotoxins (BoNTs) are potent neurotoxins and are the causative agent of botulism, as well as valuable pharmaceuticals. BoNTs are divided into seven serotypes that comprise over 40 reported subtypes. BoNT/A1 and BoNT/B1 are...
Fri, 06/07/2019 - 17:00
Maria Kusmierek, Jörn Hoßmann, Rebekka Witte, Wiebke Opitz, Ines Vollmer, Marcel Volk, Ann Kathrin Heroven, Hans Wolf-Watz, Petra Dersch Numerous Gram-negative pathogens use a Type III Secretion System (T3SS) to promote virulence injecting effector proteins into targeted host cells, which subvert host cell processes. Expression of T3SS and the effectors is triggered upon host cell contact, but...

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