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Bacterial pathogens

Tue, 01/02/2018 - 11:00
The critically endangered elkhorn coral (Acropora palmata) is affected by white pox disease (WPX) throughout the Florida Reef Tract and wider Caribbean. The bacterium Serratia marcescens was previously identified as one etiologic agent of WPX but is no longer consistently detected in contemporary outbreaks. It is now believed that multiple etiologic agents cause WPX; however, to date, no other...
Tue, 01/02/2018 - 11:00
High-pressure processing is a nonthermal method of food preservation that uses pressure to inactivate microorganisms. To ensure the effective validation of process parameters, it is important that the design of challenge protocols consider the potential for resistance in a particular species. Herein, the responses of 99 diverse Salmonella enterica strains to high pressure are reported. Members of...
Tue, 01/02/2018 - 11:00
Many pathogenic bacteria use sophisticated survival strategies to overcome harsh environmental conditions. One strategy is the formation of slow-growing subpopulations termed small colony variants (SCVs). Here we characterize an SCV that spontaneously emerged from an axenic Salmonella enterica serovar Typhimurium water culture. We found that the SCV harbored a frameshift mutation in the glutamine...
Tue, 01/02/2018 - 11:00
Heat-stable toxin (STa)-producing enterotoxigenic Escherichia coli (ETEC) strains are a top cause of moderate-to-severe diarrhea in children from developing countries and a common cause of travelers' diarrhea. Recent progress in using STa toxoids and toxoid fusions to induce neutralizing anti-STa antibodies has accelerated ETEC vaccine development. However, concern remains regarding whether the...
Tue, 01/02/2018 - 11:00
Staphylococcus aureus is part of the nasal microbiome of many humans and has become a significant public health burden due to infections with antibiotic-resistant strains, including methicillin-resistant S. aureus (MRSA) strains. Several lineages of S. aureus, including MRSA, are found in livestock species and can be acquired by humans through contact with animals. These livestock-associated MRSA...
Tue, 01/02/2018 - 11:00
Chickens with good or poor feed efficiency (FE) have been shown to differ in their intestinal microbiota composition. This study investigated differences in the fecal bacterial community of highly and poorly feed-efficient chickens at 16 and 29 days posthatch (dph) and evaluated whether a fecal microbiota transplant (FMT) from feed-efficient donors early in life can affect the fecal microbiota in...
Tue, 01/02/2018 - 00:00
Abstract This paper reports the application of an amperometric biosensor for rapid and specific Salmonella Typhimurium detection in milk. This device was developed from self-assembled monolayer technique on a gold screen-printed electrode, using cysteamine thiol. Polyclonal antibodies were oriented by protein A immobilization. The biosensor structure was characterized by cyclic...
Fri, 12/29/2017 - 20:30
Publication date: April 2018 Source:LWT, Volume 90 Author(s): Ali Aqeel Salim, Noriah Bidin, Sib Krishna Ghoshal Organic nanoparticles with controlled properties are advantageous for diversified biomedical and pharmacological applications. Cinnamon nanoparticles (CNPs) being bioactive and nontoxic can be effective antibacterial agents. Driven by this idea, we prepared spherical CNPs...
Fri, 12/29/2017 - 06:55
Publication date: June 2018 , Volume 72 Author(s): Laura Buzón-Durán, Rosa Capita, Carlos Alonso-Calleja Methicillin-resistant staphylococci (MRS) are a major concern to public and animal health. Thirty MRS (Staphylococcus aureus, S. cohnii, S. epidermidis, S. haemolyticus, S. hominis, S. lentus, S. lugdunensis, S. sciuri, and S. xylosus) isolates from meat and poultry preparations were...
Thu, 12/28/2017 - 09:41
Abstract Extraction of pigments from endophytes is an uphill task. Up till now, there are no efficient methods available to extract the maximum amount of prodigiosin from Serratia marcescens. This is one of the important endophytes of Beta vulgaris L. The present work was carried out for the comparative study of six different extraction methods such as homogenization, ultrasonication, freezing...
Thu, 12/28/2017 - 06:35
Abstract The study aimed to determine genetic diversity of a collection of New Zealand Cronobacter isolates collected from different sources and their ability to form a biofilm on different materials under different conditions. Isolates were screened for the presence of biofilm associated genes. Furthermore, suppression subtractive hybridization (SSH) was used to identify unique genes, present in...
Thu, 12/28/2017 - 05:30
Abstract The effect of microbial control measures in farmstead cheese making was evaluated in a small dairy farm in Gyeong-gi province, Korea. Teat washing and pasteurization significantly (p < .05) reduced the level of indicator bacteria (mesophilic aerobes, total coliforms, and Escherichia coli). However, farmstead cheeses were contaminated with increased levels of mesophilic aerobes, total...
Thu, 12/28/2017 - 03:00
Journal of Food Protection, Volume 81, Issue 1, Page 142-149, January 2018.
Thu, 12/28/2017 - 03:00
Journal of Food Protection, Volume 81, Issue 1, Page 150-157, January 2018.
Wed, 12/27/2017 - 19:00
Kazakhstan belongs to countries with a high level of brucellosis among humans and farm animals. Although antibiotic therapy is the main way to treat acute brucellosis in humans there is still little informatio...
Wed, 12/27/2017 - 18:50
Abstract Seafood has often been implicated in outbreaks of food-borne illness caused by Listeria monocytogenes but the source of contamination is usually not known. In this study we investigated the possibility that this pathogen could survive in seawater for an extended time period. Freshly collected seawater samples were inoculated with 1 x 108 CFU ml−1 of L. monocytogenes EGD-e and survival...
Wed, 12/27/2017 - 17:00
Olga Moreno-Gonzalo, Marta Ramírez-Huesca, Noelia Blas-Rus, Danay Cibrián, María Laura Saíz, Inmaculada Jorge, Emilio Camafeita, Jesús Vázquez, Francisco Sánchez-Madrid Recent evidence on HDAC6 function underlines its role as a key protein in the innate immune response to viral infection. However, whether HDAC6 regulates innate immunity during bacterial infection remains unexplored. To assess...
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): Zi Teng, Yaguang Luo, Solmaz Alborzi, Bin Zhou, Lin Chen, Jinglin Zhang, Boce Zhang, Patricia Millner, Qin Wang Chlorine, the most commonly used sanitizer for fresh produce washing, has constantly shown inferior sanitizing efficacy in the presence of organic load. Conventionally this is attributed indirectly to the...
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): James De Souza, Rafath Ahmed, Philip Strange, Shai Barbut, S. Balamurugan Dry fermented sausages (DFS) have been subject to numerous validation studies, as pathogen reduction heavily relies on both ingredients and processing. In this study the effect of product caliber size (32, 55, 80mm), and fat level (low, 9.67%; high, 18....
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): Marilyn C. Erickson, Jye-Yin Liao, Cathy C. Webb, Mussie Y. Habteselassie, Jennifer L. Cannon Gloves are worn by workers harvesting ready-to-eat produce as a deterrent for contaminating the produce with enteric pathogens that may reside on their hands. As fields are not sterile environments, the probability for gloves to...
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): Elena Sorrentino, Mariantonietta Succi, Luca Tipaldi, Gianfranco Pannella, Lucia Maiuro, Marina Sturchio, Raffaele Coppola, Patrizio Tremonte Refrigeration alone or in combination with other technologies represents the main tool used in the last decades to preserve the freshness of black truffles. This is principally...
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): Meng Wang, Junjie Yang, Zhongtao Gai, Shengnan Huo, Jianhua Zhu, Jun Li, Ranran Wang, Sheng Xing, Guosheng Shi, Feng Shi, Lei Zhang As a kind of zero-tolerance foodborne pathogens, Salmonella typhimurium poses a great threat to quality of food products and public health. Hence, rapid and efficient approaches to...
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): Sudarsan Mukhopadhyay, Kimberly Sokorai, Dike O. Ukuku, Tony Jin, Xuetong Fan, Modesto Olanya, Vijay Juneja The objective of this study was to evaluate inactivation of inoculated Salmonella enterica on grape tomato stem scars exploiting integrated treatment of organic acid wash (AW) followed by chitosan-allyl...
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): Beyhan Gunaydin Dasan, Tugba Yildirim, Ismail Hakki Boyaci In this study, the possibility of using an effective short time non-thermal plasma (NTP) treatment to inactivate Salmonella enterica serovar Enteritidis on eggshell surface was investigated. The eggshells were artificially contaminated with S. Enteritidis at an initial...
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): Lihui Zhang, Xiaoxi Kou, Shuang Zhang, Teng Cheng, Shaojin Wang Water activity (a w ) and heating rate have shown important effects on the thermo-tolerance of pathogens in low moisture foods during thermal treatments. In this study, three strains were selected to compare the heat resistance in walnut shell powder and finally...
Wed, 12/27/2017 - 14:51
Publication date: 2 February 2018 , Volume 266 Author(s): Nguyen Thi Nhung, Nguyen Thi Bich Van, Nguyen Van Cuong, Truong Thi Quy Duong, Tran Thi Nhat, Tran Thi Thu Hang, Nguyen Thi Hong Nhi, Bach Tuan Kiet, Vo Be Hien, Pham Thi Ngoc, James Campbell, Guy Thwaites, Juan Carrique-Mas Excessive antimicrobial usage and deficiencies in hygiene in meat production systems...
Wed, 12/27/2017 - 06:15
Abstract AimsDairy cattle shed pathogenic E. coli O157 (O157) in feces, playing a role in human exposure. We aimed to measure fecal microbial communities in early lactation dairy cattle, and model outcomes with O157 shedding metrics. Methods and ResultsDaily fecal samples were collected from 40 cattle on two Colorado dairies for five consecutive days, and characterized for O157. 16S rRNA gene...
Wed, 12/27/2017 - 06:06
Abstract AimsThe purpose of this survey was to estimate the respective prevalence of the “gang of seven” and “non-gang of seven” serotypes of Shigatoxigenic and enteropathogenic Escherichia coli and to identify the O80:H2 serotype in 245 intestinal contents collected at two slaughterhouses in Belgium in 2014. Methods and ResultsAfter overnight enrichment growth the 69 intestinal contents testing...
Tue, 12/26/2017 - 18:21
Publication date: Available online 26 December 2017 Author(s): Abbey J. Steckler, Maria X. Cardenas-Alvarez, Megan K. Townsend Ramsett, Neil Dyer, Teresa M. Bergholz Listeria monocytogenes infections are an important disease of ruminants worldwide, causing encephalitis, septicemia, and abortions. Ruminant listeriosis can also pose a food safety risk due to the potential for L....
Tue, 12/26/2017 - 11:00
Conventional methods for the identification of gastrointestinal pathogens are time-consuming and expensive and have limited sensitivity. The aim of this study was to determine the clinical impact of a comprehensive molecular test, the BioFire FilmArray gastrointestinal (GI) panel, which tests for many of the most common agents of infectious diarrhea in approximately 1 h. Patients with stool...
Sun, 12/24/2017 - 20:25
Publication date: April 2018 Source:LWT, Volume 90 Author(s): Morgan A. Redemann, Jagpinder Brar, Steven E. Niebuhr, Lisa M. Lucia, Gary R. Acuff, James S. Dickson, Manpreet Singh The United States Department of Agricultures’ Food Safety and Inspection Service (USDA-FSIS) has developed thermal lethality guidelines for non-typhoidal Salmonella inactivation in ready-to-eat (RTE)...
Sun, 12/24/2017 - 13:06
Publication date: Available online 24 December 2017 Food Science and Human Wellness Author(s): Lu Lin, Jie Yun Hu, Yi Wu, Min Chen, Jie Ou, Wei Ling Yan This study was conducted to analyze the effects of sodium nitrite, nisin, potassium sorbate, and sodium lactate against Staphylococcus aureus(S. aureus) growth and staphylococcal enterotoxins(SEs) production in cooked pork sausage...
Sun, 12/24/2017 - 05:55
Publication date: June 2018 , Volume 72 Author(s): Simen Akkermans, Jan F. Van Impe Modelling and simulation of microbial dynamics as a function of processing, transportation and storage conditions is a useful tool to improve microbial food safety and quality. The goal of this research is to improve an existing methodology for building mechanistic predictive models based on the...
Sat, 12/23/2017 - 04:15
Publication date: April 2018 , Volume 86 Author(s): Teng Cheng, Shaojin Wang The purposes of this study were to explore combined effects of storage temperature, time and atmosphere on survival and thermal inactivation of pathogenic Salmonella Enteritidis PT 30 with nonpathogenic E. coli ATCC 25922 as a surrogate inoculated to low-moisture almond powder. A thermal death time test heating...
Sat, 12/23/2017 - 04:15
Publication date: April 2018 , Volume 86 Author(s): Lanhua Yi, Lingli Luo, Xin Lü Foodborne pathogens cause diseases by food chain transmission, therefore preservatives or antimicrobials are essential for food products, among which bacteriocins are considered as promising alternatives of chemical preservatives. Moreover, bacteriocins can potentially be used as antibiotic alternatives....
Sat, 12/23/2017 - 04:15
Publication date: April 2018 , Volume 86 Author(s): Alonzo A. Gabriel, Ma. Luisa P. Ballesteros, Leo Mendel D. Rosario, Roy B. Tumlos, Henry J. Ramos This study aimed to evaluate the efficacy of ultraviolet-C irradiation and atmospheric pressure plasma jet treatment as inactivation methods of Salmonella spp. on stainless steel surfaces commonly used as food contact surfaces. Two...
Sat, 12/23/2017 - 04:15
Publication date: April 2018 , Volume 86 Author(s): Yen-Shan Huang, Cheng-An Hwang, Lihan Huang, Vivian Chi-Hua Wu, Hsin-I Hsiao The steadily increased consumption of raw oysters in Taiwan warrants an assessment of the risk (probability of illness) of raw oyster consumption attributed by Vibrio parahaemolyticus. The aim of this study was to estimate the risk of V. parahaemolyticus...
Sat, 12/23/2017 - 02:05
Summary Increased consumption of produce by consumers has been attributed to perceived health benefits of postharvest produce. Pathogen control is crucial because periodic occurrences and contamination of tomato and leafy greens have exacerbated food safety risks for consumers. We investigated the effects of temperatures (5 and 25 °C), storage time (30 min and 24 h) for inactivation of Listeria...
Fri, 12/22/2017 - 17:00
Claudia Jäckel, Jens A. Hammerl, Jörg Rau, Stefan Hertwig Campylobacter jejuni and C. coli are important food-borne pathogens that are widespread in animal husbandry. To combat Campylobacter along the food chain, the application of lytic phages has been shown to be a promising tool. Campylobacter phages are currently classified into three groups, of which group II and group III phages are the...
Fri, 12/22/2017 - 14:30
Publication date: 21 February 2018 , Volume 267 Author(s): Jing Chen, Bosoon Park Immunomagnetic separation (IMS) as a culture-free enrichment sample preparation technique has gained increasing popularity in the development of rapid detection methods for foodborne pathogens. While the use of magnetic nanoparticles in IMS is on the rise due to substantially larger surface area compared to...
Fri, 12/22/2017 - 03:00
Journal of Food Protection, Volume 81, Issue 1, Page 79-83, January 2018.
Fri, 12/22/2017 - 00:00
Abstract Vibrio parahaemolyticus is part of the natural microflora of estuarine and coastal marine waters and can be also present in seafood, especially shellfish and bivalve molluscs. In this study we compared the reference cultural method ISO 6887-3 with two molecular methods, multiplex PCR and real-time PCR, for the detection of two distinct...
Thu, 12/21/2017 - 17:00
Ryosuke Goto, Tsuyoshi Miki, Nao Nakamura, Mayuka Fujimoto, Nobuhiko Okada Mucosal barrier formed cationic antimicrobial peptides (CAMPs) is believed to be crucial for host protection from pathogenic gut infection. However, some pathogens can develop resistance to the CAMPs to survive in hosts. Salmonella enterica is a common cause of acute diarrhea. During the course of this disease, the...
Thu, 12/21/2017 - 14:24
Publication date: Available online 21 December 2017 Author(s): Anastasia E. Lytou, George-John E. Nychas, Efstathios Z. Panagou Pomegranate juice is a product with enhanced functional properties that could be used as an alternative to traditional marination ingredients and effectively retard microbial growth along with providing an improved sensory result. In this study, two...
Thu, 12/21/2017 - 11:30
AbstractThe present study tested antibacterial activity of a rice hull smoke extract (RHSE) against a multidrug-resistant strain of Salmonella Typhimurium and examined its mode of suppressive action in vitro and in mice. In vitro studies showed that the minimum inhibitory concentration (MIC) value of RHSE was 1.29% (v/v). The inactivation was confirmed by complete loss of cell viability in the...
Thu, 12/21/2017 - 01:16
Publication date: March 2018 , Volume 105 Author(s): Xinyu Liao, Jiao Li, Yuanjie Suo, Juhee Ahn, Donghong Liu, Shiguo Chen, Yaqin Hu, Xingqian Ye, Tian Ding As the development of hurdle technology, cross-protection of various stresses for pathogens posed the potential risk to food safety and public health. This study tried to explore various preliminary stresses including...

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