Bacterial pathogens

Sat, 06/19/2021 - 08:06
by Tatjana Reuter, Felix Scharte, Rico Franzkoch, Viktoria Liss, Michael Hensel Salmonella enterica is a common foodborne, facultative intracellular enteropathogen. Human-restricted typhoidal S. enterica serovars Typhi (STY) or Paratyphi A (SPA) cause severe typhoid or paratyphoid fever, while many S. enterica serovar Typhimurium (STM) strains have a broad host range and in human hosts usually...
Sat, 06/19/2021 - 06:33
Author(s): Eswaramoorthy Thirumalaikumar, Chinnadurai Lelin, Ramamoorthy Sathishkumar, Sugumar Vimal, Setty Balakrishnan Anand, Mariavincent Michael Babu, Thavasimuthu Citarasu
Sat, 06/19/2021 - 06:20
LWT
Author(s): Daniela Comparsi Laranja, Patrícia da Silva Malheiros, Fabíola Ayres Cacciatore, Susana de Oliveira Elias, Bruno Pinheiro Milnitsky, Eduardo Cesar Tondo
Sat, 06/19/2021 - 04:02
Ubiquity of Listeria monocytogenes in the environment impacts the food industry and presents concerns for frozen food facilities. This study determined the prevalence and numbers of Listeria species and L. monocytogenes on raw produce arriving at frozen food facilities. Raw produce was collected using multi-level blinding protocols to ensure anonymity of participants and avoid traceback. Five raw...
Sat, 06/19/2021 - 03:01
As part of the ongoing studies with clinically relevant   Klebsiella   spp., we characterized the genomes of three clinical GES-5-positive ST138 strains originally identified as Klebsiella oxytoca. bla OXY gene, average nucleotide identity and phylogenetic analyses showed the strains to be   Klebsiella michiganensis  . Affiliation of the strains to ST138...
Sat, 06/19/2021 - 00:00
Post-stroke spasticity impedes patients' rehabilitation progress. Contradictory evidence has been reported in using Botulinum Neurotoxin type A (BoNT-A) to manage post-stroke lower extremity spasticity (PLES); furthermore, an optimum dose of BoNT-A for PLES has not yet been established. Therefore, we conducted a systematic review and meta-analysis of randomized controlled trials (RCTs) to...
Fri, 06/18/2021 - 16:00
The aim of the study was to evaluate the genotypic and phenotypic characteristics of 20 strains of S. Heidelberg (SH) isolated from broilers produced in southern Brazil. The similarity and presence of genetic determinants linked to virulence, antimicrobial resistance, biofilm formation, and in silico-predicted metabolic interactions revealed this serovar as a threat to public health. The presence...
Fri, 06/18/2021 - 16:00
The problem of antibiotic resistance has prompted researchers around the globe to search for new antimicrobial agents. Antimicrobial proteins and peptides are naturally secreted by almost all the living organisms to fight infections and can be safer alternatives to chemical antibiotics. Lactoferrin (LF) is a known antimicrobial protein present in all body secretions. In this study, LF was...
Fri, 06/18/2021 - 16:00
Lactobacillus casei (L. casei), a normal resident of the gastrointestinal tract of mammals, has been extensively studied over the past few decades for its probiotic properties in clinical and animal models. Some studies have shown that some bacterium of Lactobacillus stimulate the production of antimicrobial peptides in intestinal cells to clear enteric pathogens, however, which antimicrobial...
Fri, 06/18/2021 - 16:00
The midgut microbiota of the yellow fever mosquito Aedes aegypti impacts pathogen susceptibility and transmission by this important vector species. However, factors influencing the composition and size of the microbiome in mosquitoes are poorly understood. We investigated the impact of larval diet abundance during development on the composition and size of the larval and adult microbiota by...
Fri, 06/18/2021 - 08:03
by Ho Jae Lim, Eun-Rim Kang, Min Young Park, Bo Kyung Kim, Min Jin Kim, Sunkyung Jung, Kyoung Ho Roh, Nackmoon Sung, Jae-Hyun Yang, Min-Woo Lee, Sun-Hwa Lee, Yong-Jin Yang Classification of clinical symptoms and diagnostic microbiology are essential to effectively employ antimicrobial therapy for lower respiratory tract infections (LRTIs) in a timely manner. Empirical antibiotic treatment without...
Fri, 06/18/2021 - 06:13
Author(s): Xue Qin, Hao Wang, Chao Miao, Xinyan Yang, Yanming Zhang, Jing Feng, Stephen J. Forsythe, Chaoxin Man, Yujun Jiang
Fri, 06/18/2021 - 02:04
Abstract As the reality of pandemic threats challenges humanity, exemplified during the ongoing SARS-CoV-2 infections, the development of vaccines targeting these etiological agents of disease has become increasingly critical. Of paramount concern are novel and reemerging pathogens that could trigger such events, including the plague bacterium Yersinia pestis. Y. pestis is responsible for more...
Fri, 06/18/2021 - 02:02
The quality of water resources can be altered by human activities carried out in watersheds. These changes can lead to the occurrence of antibiotic-resistant bacteria and compromise public health. The aim of the present study was to evaluate the presence and concentration of total coliforms and Escherichia coli in the water at the Ecological Park Tietê in São Paulo, the antibiotics resistance of...
Fri, 06/18/2021 - 00:00
Carbapenem-resistant Enterobacteriaceae (CRE), especially carbapenem-resistant Klebsiella pneumoniae (CRKP), are among the largest pathogenic threats to humans. The available antibiotic treatment options for combating CRKP are limited. Colistin-resistant Enterobacteriaceae (CoRE) have also been reported worldwide, including in Thailand. Therefore, this study aimed 1) to determine minimum...
Thu, 06/17/2021 - 16:00
Vibrio alginolyticus is one of the most important of pathogens that can infect humans and a variety of aquatic animals, and it can cause food poisoning and septicemia in humans. Widely used antibiotics are gradually losing their usefulness, and phages are gaining more attention as new antibacterial strategies. To have more potential strategies for controlling pathogenic bacteria, we isolated a...
Thu, 06/17/2021 - 08:06
by Cristina Giogha, Nichollas E. Scott, Tania Wong Fok Lung, Georgina L. Pollock, Marina Harper, Ethan D. Goddard-Borger, Jaclyn S. Pearson, Elizabeth L. Hartland During infection, enteropathogenic Escherichia coli (EPEC) and enterohaemorrhagic E. coli (EHEC) directly manipulate various aspects of host cell function through the translocation of type III secretion system (T3SS) effector proteins...
Thu, 06/17/2021 - 08:06
This observational study determined the lipidome of cow milk during subclinical intramammary infection (IMI) by non-aureus staphylococci (NAS), also defined as coagulase-negative staphylococci, using an untargeted approach. Among the pathogens causing bovine IMI, NAS have become the most frequently isolated bacteria from milk samples. Although the application of system biology approaches to...
Thu, 06/17/2021 - 06:19
LWT
Author(s): Cid R. González-González, Olaoniye Labo-Popoola, Gonzalo Delgado-Pando, Katerina Theodoridou, Olena Doran, Alexandros Ch Stratakos In this study, the antimicrobial efficiency of a novel low-voltage piezoelectric direct discharge generated cold plasma (CAP) and linalool-loaded nanoemulsion washing (LW) was assessed against Salmonella and Escherichia coli O157:H7 on ready-to-eat (RTE)...
Thu, 06/17/2021 - 00:00
Metal-based drugs, including lanthanide complexes, have been extremely effective in clinical treatments against various diseases and have raised major interest in recent decades. Hence, in this work, a series of lanthanum (III) and cerium (III) complexes, including Schiff base ligands derived from (1H-benzimidazol-2-yl)aniline, salicylaldehyde, and 2,4-dihydroxybenzaldehyde were synthesized and...
Wed, 06/16/2021 - 22:11
Journal of the World Aquaculture Society, EarlyView. To evaluate the protective effect of baicalin against a Vibrio parahaemolyticus challenge, immune parameters were analyzed and RNA interference (RNAi) was performed in the present study. The results from challenge tests showed that the cumulative mortalities in the 200 and 400 mg/kg baicalin groups after challenge with V. parahaemolyticus were...
Wed, 06/16/2021 - 16:00
Background: Enabling faster Antimicrobial Susceptibility Testing (AST) is critical, especially to detect antibiotic resistance, to provide rapid and appropriate therapy and to improve clinical outcomes. Although several standard and automated culture-based methods are available and widely used, these techniques take between 18 and 24 h to provide robust results. Faster techniques are needed to...
Wed, 06/16/2021 - 16:00
The purpose of this study was to investigate the prevalence, antimicrobial resistance, virulence genes, and genetic diversity of Campylobacter spp. along the yellow-feathered broiler slaughtering line in Southern China from December 2018 to June 2019. A total of 157 Campylobacter spp. isolates were identified from 1,102 samples (including 53.6% (75/140) of live chicken anal swab samples, 27.5% (...
Wed, 06/16/2021 - 12:01
Citrobacter freundii is a significant cause of human infections, responsible for food poisoning, diarrhea, and urinary tract infections. We previously identified a highly cytotoxic and adhesive C. freundii strain CF74 expressing a type VI secretion system (T6SS). In this study, we showed that in mice-derived macrophages, C. freundii CF74 activated the Nucleotide Oligomerization Domain -Like...
Wed, 06/16/2021 - 04:03
Fresh Milk Bar (FMB), an emerging dairy retail franchise, is used to instantly produce and sell pasteurized milk and other dairy products in China. However, the quality and safety of pasteurized milk in FMB have received little attention. The objective of this study is to investigate the prevalence, antimicrobial resistance, and virulence genes of Escherichia coli (E. coli) , Staphylococcus...
Wed, 06/16/2021 - 04:03
Experiments were performed to assess the survival of Salmonella on whole Brazil nuts and pumpkin seeds stored at 8ºC, 23ºC, and 37ºC. Brazil nut kernels and pumpkin seeds were inoculated with bacterial inoculum containing 10.4 Log 10 CFU/ml of Salmonella Enteritidis Phage Type 30 ( S E PT30) and then aseptically dried at room temperature for 24 hrs. After the drying step, levels of Salmonella...
Wed, 06/16/2021 - 03:01
Specific lineages of serogroup O1 Vibrio cholerae are notorious for causing cholera pandemics, of which there have been seven since the 1800s. Much is known about the sixth pandemic (1899–1923) and the ongoing seventh pandemic (1961–present), but we know very little about the bacteriology of pandemics 1 to 5. Moreover, although we are learning about the contribution of non-O1 non-...
Wed, 06/16/2021 - 02:02
This research was carried out to investigate the differences in adhesion and growth during biofilm formation of L. monocytogenes from different sources and clonal complexes. Biofilm by L. monocytogenes (isolates CLIST 441 and 7: both lineage I, serotype 1/2b, CC3; isolates 19 and 508: both lineage II, serotype 1/2c, CC9) was grown on stainless steel coupons under different stressing conditions (...
Wed, 06/16/2021 - 00:00
The aim of this study was to demonstrate an increase in muscle action potentials and an enhancement of the efficacy of botulinum toxin (BoNT) after mechanical leg vibration. Methods: A 53-year-old healthy male volunteer underwent vibration ergometry training (VET) every morning and every evening for 10 min for 14 days. Compound muscle action potential (CMAP) of the right (R) and left (L) extensor...
Tue, 06/15/2021 - 16:00
The transfer of the intestinal microbiota from adult to juvenile animals reduces Salmonella prevalence and abundance. The mechanism behind this exclusion is unknown, however, certain member species may exclude or promote pathogen colonization and Salmonella abundance in chickens correlates with intestinal community composition. In this study, newly hatched chicks were colonized with Salmonella...
Tue, 06/15/2021 - 16:00
The mcr-1 gene mediating mobile colistin resistance in Escherichia coli was first reported in China in 2016 followed by reports among different species worldwide, especially in E. coli and Klebsiella. However, data on its transmission in Salmonella are still lacking. This study analyzed the antimicrobial resistance (AMR) profiles and the mcr-1 gene presence in 755 foodborne Salmonella from 26...
Tue, 06/15/2021 - 16:00
In this study, a detailed genetic dissection of the huge and complex blaNDM-carrying genetic elements and their related mobile genetic elements was performed in Enterobacteriaceae. An extensive comparison was applied to 12 chromosomal genetic elements, including six sequenced in this study and the other six from GenBank. These 12 genetic elements were divided into five groups: a novel IME Tn6588...
Tue, 06/15/2021 - 08:05
The type 6 secretion system (T6SS) is a bacterial weapon broadly distributed in gram-negative bacteria and used to kill competitors and predators. Featuring a long and double-tubular structure, this molecular machine is energetically costly to produce and thus is likely subject to diverse regulation strategies that are largely ill defined....
Tue, 06/15/2021 - 02:03
Abstract Tuberculosis (TB) caused by Mycobacterium tuberculosis (M. tuberculosis) is a fatal infectious disease to human health, and the drug tolerance and immune evasion of M. tuberculosis were reported to be related to its biofilm formation; however, the difficulty of M. tuberculosis biofilm culture and its unknown global mechanism impede its further research. Here, we developed a modified in...
Mon, 06/14/2021 - 16:00
Brucella, a notorious intracellular pathogen, causes chronic infections in many mammals, including humans. The twin-arginine translocation (Tat) pathway transports folded proteins across the cytoplasmic membrane; protein substrates translocated by Brucella include ABC transporters, oxidoreductases, and cell envelope biosynthesis proteins. Previously, we showed that a Tat mutant of Brucella...
Mon, 06/14/2021 - 06:08
LWT
Author(s): Jessica Audrey Feijó Corrêa, João Vitor Garcia dos Santos, Alberto Gonçalves Evangelista, Anne Caroline Schoch Marques Pinto, Renata Ernlund Freitas de Macedo, Fernando Bittencourt Luciano Essential oil components (EOCs) show great potential for substitution of classic synthetic preservatives in the food industry, but their intense flavor at high concentrations hinders the commercial...
Mon, 06/14/2021 - 00:00
Bacterial ghosts (BGs) are empty cell envelopes possessing native extracellular structures without a cytoplasm and genetic materials. BGs are proposed to have significant prospects in biomedical research as vaccines or delivery carriers. The applications of BGs are often limited by inefficient bacterial lysis and a low yield. To solve these problems, we compared the lysis efficiency of the wild-...
Sun, 06/13/2021 - 06:08
LWT
Author(s): Jiajia Wan, Liping Zheng, Liangyu Kong, Zhaoxin Lu, Yang Tao, Zhiyang Feng, Fengxia Lv, Fanqiang Meng, Xiaomei Bie Two real-time quantitative PCR detection systems that contained DNA Green saturated fluorescent dye for detection of Salmonella Enteritidis and Ly Green saturated fluorescent dye for detection of Salmonella spp. were constructed. Furthermore, the specificity of the...
Sun, 06/13/2021 - 02:03
Abstract This study is a comparative investigation of antioxidant and antibacterial properties of tree bark extracts of three common European species, Prunus avium L., Larix decidua Mill. and Castanea sativa Mill. The bioactive compounds present in the bark were recovered in 80% aqueous ethanol using ultrasound as the green extraction method. The antioxidant potential of the extracts was assessed...
Sun, 06/13/2021 - 00:00
Heat-labile toxin I (LT-I), produced by strains of enterotoxigenic Escherichia coli (ETEC), causes profuse watery diarrhea in humans. Different in vitro and in vivo models have already elucidated the mechanism of action of this toxin; however, their use does not always allow for more specific studies on how the LT-I toxin acts in systemic tracts and intestinal cell lines. In the present work,...
Sun, 06/13/2021 - 00:00
Staphylococcus (S.) aureus is an important causative agent of wound infections with increasing incidence in the past decades. Specifically, the emergence of methicillin-resistant S. aureus (MRSA) causes serious problems, especially in nosocomial infections. Therefore, there is an urgent need to develop of alternative or supportive antimicrobial therapeutic modalities to meet these challenges....
Sun, 06/13/2021 - 00:00
The recurrent emergence of infection outbreaks associated with shellfish consumption is of extreme importance for public health. The present study investigated the potential application of phages AH-1, AH-4, and AH-5 to inactivate Aeromonas hydrophila, a causative agent of infections in humans associated with bivalve shellfish consumption. The inactivation of A. hydrophila was assessed in vitro,...
Sat, 06/12/2021 - 08:05
by Nuntita Singpanomchai, Yukihiro Akeda, Kazunori Tomono, Aki Tamaru, Pitak Santanirand, Panan Ratthawongjirakul Multidrug-resistant tuberculosis (MDR-TB) poses a serious threat to TB control. Early diagnosis and proper treatment are essential factors to limit the spread of the disease. The existing molecular tests for MDR-TB usually require specific instruments, steady power supply, and routine...
Sat, 06/12/2021 - 04:02
L. monocytogenes causes relatively few outbreaks linked to whole fresh produce but triggers recalls each year in the US. There are limited data on the influence of wet vs. dry methods on pathogen growth on whole produce. A cocktail of five L. monocytogenes strains that included clinical, food, or environmental isolates associated with foodborne outbreaks and recalls was used. Cultures were...
Sat, 06/12/2021 - 04:02
The application of plant extracts (PEs) could be a promising option to satisfy consumers’ demand for natural additives to inhibit growth of variable pathogenic bacteria. Thus, the aim of this study was to develop a standardized microdilution method to examine the antimicrobial effects of ten hydrophilic plant extracts against two strains of C. perfringens facing various food-relevant influencing...
Sat, 06/12/2021 - 03:01
Objective. This cross-sectional study conducted in Kibera, Kenya, sought to gain insights on relative microbial contamination levels of popular unprocessed food types, determine antimicrobial resistance (AMR) burden and the carriage of integrons that are essential elements for spreading antimicrobial resistance genes (ARG). Foods analysed consisted of cooked vegetables (kale, cabbage, and...
Sat, 06/12/2021 - 00:00
Escherichia coli (EHEC) and Shigella dysenteriae serotype 1 are enterohemorrhagic bacteria that induce hemorrhagic colitis. This, in turn, may result in potentially lethal complications, such as hemolytic uremic syndrome (HUS), which is characterized by thrombocytopenia, acute renal failure, and neurological abnormalities. Both species of bacteria produce Shiga toxins (Stxs), a phage-encoded...
Fri, 06/11/2021 - 22:09
Journal of Fish Diseases, EarlyView. A rapid increase in multi-drug-resistant (MDR) bacteria in aquaculture highlights the risk of production losses due to diseases and potential public health concerns. Previously, we reported that ozone nanobubbles (NB-O3) were effective at reducing concentrations of pathogenic bacteria in water and modulating fish immunity against pathogens; however, multiple...
Fri, 06/11/2021 - 16:00
Since sewage is a hotspot for antibiotic resistance genes (ARGs), the identification of ARGs in environmental waters impacted by sewage, and their correlation to fecal indicators, is necessary to implement management strategies. In this study, sewage treatment plant (STP) influent samples were collected and analyzed using quantitative polymerase chain reaction (qPCR) to investigate the abundance...
Fri, 06/11/2021 - 06:14
Author(s): Yurong Zhu, Ye Lu, Lin Yuan, Wei Ling, Xugan Jiang, Shengxia Chen, Bing Hu Listeria monocytogenes (Lm) can lead to high mortality rates relative to other foodborne pathogens. Lm-induced inflammation is partly characterized by macrophage activation. Long non-coding RNAs (lncRNAs) have important roles in various biological processes. However, it is unknown how lncRNAs regulate the host...

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