Bacterial pathogens

Thu, 04/09/2020 - 03:39
ABSTRACT Carbapenemase-producing Enterobacterales (CPE) have become an important public health concern. In our hospital, VIM enzymes were first detected in 2005, Klebsiella pneumoniae carbapenemase (KPC) enzymes in 2009, and OXA-48 enzymes in 2012. We assess the population biology of the first OXA-48-producing Enterobacterales isolates recovered in our hospital (2012 to 2013) where infections by...
Thu, 04/09/2020 - 03:35
Q fever, caused by the zoonotic bacterium Coxiella burnetii, is a globally distributed emerging infectious disease. Livestock are the most important zoonotic transmission sources, yet infection in people without livestock exposure is common. Identifying potential exposure pathways is necessary to design effective interventions and aid outbreak prevention. We used natural language processing and...
Thu, 04/09/2020 - 03:34
High oleic peanuts were inoculated with Enterococcus faecium and roasted in a convection oven at 190°C. There was a 6 log CFU/g reduction at 480 s. D‐values for high oleic peanut butter at 110, 120, and 125°C were 438.9, 165.1, and 80.6 s, respectively. The z‐value was calculated to be 20.8°C. High oleic peanut butter was inoculated with E. faecium and agitated in a heated mixer for 21.5 min. E....
Thu, 04/09/2020 - 03:32
Our data suggest a model in which L. monocytogenes ΔspxA1 produces less cytochrome bd than wt, generating ROS from the incomplete electron transport chain. This would normally be readily detoxified but ΔspxA1 is deficient in catalase production and is thus more sensitive to peroxide‐mediated toxicity. Therefore, the severe ΔspxA1 aerobic growth defect is the result of increased ROS in the absence...
Thu, 04/09/2020 - 03:32
Aims The food supply is a potential source of antimicrobial resistance. Current surveillance programs targeting food are limited to beef, pork and poultry and do not capture niche products. In this study, imported reptile and amphibian products were screened for antimicrobial resistant bacteria. Methods and Results Fifty‐three items including soft shell turtles, frog legs, geckos, snakes and a...
Thu, 04/09/2020 - 03:30
The growth of Listeria monocytogenes on refrigerated, ready-to-eat food products is a major health and economic concern. The natural antimicrobial nisin targets the bacterial cell wall and can be used to inhibit L. monocytogenes growth on cheese. Cell wall composition and structure, and therefore the efficacy of cell wall acting control strategies, can be severely affected by environmental and...
Thu, 04/09/2020 - 03:30
Development of probiotic-ineffective antimicrobial peptides (AMPs)-based coatings that can kill pathogenic bacteria at low concentrations but are essentially harmless (even high concentrations) to probiotic organisms is a relatively new trend for therapy against GI tract infections. In this study, a series of triplet-tryptophan-pivot peptides with various hydrophilic amino acids was constructed....
Thu, 04/09/2020 - 03:30
Microbiological surveillance of the food chain plays a critical role in improving our understanding of the distribution and circulation of food-borne pathogens along the farm to fork continuum toward the development of interventions to reduce the burden of illness. The application of molecular subtyping to bacterial isolates collected through surveillance has led to the identification of strains...
Thu, 04/09/2020 - 03:30
Viable pathogenic bacteria are major biohazards that pose a significant threat to food safety. Despite the recent developments in detection platforms, multiplex identification of viable pathogens in food remains a major challenge. A novel strategy is developed through direct metatranscriptome RNA-seq and multiplex RT-PCR amplicon sequencing on Nanopore MinION to achieve real-time multiplex...
Thu, 04/09/2020 - 03:30
Autoinducer-2 (AI-2) has a widely accepted role in bacterial intra- and interspecies communication. Little is known about the relationships between AI-2 and NEC. This study found that AI-2 levels in patients and in a NEC mouse model were detected using the Vibrio harveyi BB170 assay system. Bacterial communities of the newborns' stool microbiota (NEC acute group, NEC recovery group, control group...
Thu, 04/09/2020 - 03:30
Shigella sonnei is a rod-shaped, Gram-negative facultative intracellular pathogen. It was named ‘Sonne’s bacillus’ after Carl Olaf Sonne who described it as a causative agent of bacillary dysentery. S. sonnei is distributed worldwide and represents the most common cause of shigellosis in industrialized regions in Europe, North America, and Australia. It is currently undergoing expansion in middle...
Wed, 04/08/2020 - 03:44
Vibrio parahaemolyticus is a widespread bacterium in the marine environment and is one of the leading causes of food-derived bacterial poisoning in humans worldwide. The main objective of this study was to determine the prevalence, virulence factors and antibiotic and heavy metal resistance profiles of V. parahaemolyticus in Pacific mackerel from different markets in Zhejiang, People’s Republic...
Wed, 04/08/2020 - 03:42
Author(s): Isabella de Medeiros Barbosa, Érika Tayse da Cruz Almeida, Ana Cristina Alves Gomes, Evandro Leite de Souza Induction of viable but non-culturable (VBNC) state in Listeria monocytogenes by Origanum vulgare L. (OVEO) and Rosmarinus officinalis L. essential oils (ROEO) in a meat-based broth and phosphate buffered saline was investigated. Occurrence of culturability loss on brain-heart...
Wed, 04/08/2020 - 03:42
Author(s): Shulin Liu, Aiguo Zhou, Shaolin Xie, Di Sun, Yue Zhang, Zhuolin Sun, Yanfeng Chen, Jixing Zou The great Gambusia affinis (G. affins) is considered as a important animal model to study the endocrine disruption, ecological behavior, and environmental pollutant. The present study aims to build a new promising infection model with A. hydrophila in aquaculture. The mRNA expression of Rac1,...
Wed, 04/08/2020 - 03:36
The membrane‐bound transcription regulator ToxR controls housekeeping and virulence gene expression in V. cholerae. The activity and stability of ToxR strongly depends on its operon partner ToxS, the redox state of its periplasmic cysteine residues and environmental stimuli. Here, ToxR‐ToxR PPIs are key for its activity, which is influenced by ToxS, DNA binding capabilities, bile and the...
Wed, 04/08/2020 - 03:33
Yersinia enterocolitica exhibits a dual lifestyle, existing as both a saprophyte and a pathogen colonizing different niches within a host organism. OmpR has been recognized as a regulator that controls the expression of genes involved in many different cellular processes and the virulence of pathogenic bacteria. Here, we have examined the influence of OmpR and varying temperature (26°C vs. 37°C)...
Wed, 04/08/2020 - 03:33
The effects of previous Salmonella Typhimurium habituation to an Italian-style salami concerning pathogen resistance against ultraviolet-C light (UV-C) treatment were modeled in order to establish treatment feasibility for the decontamination of dry-fermented sausage. S. Typhimurium following 24 h habituation in fermented sausage (habituated cells) or non-habituation (non-habituated cells) were...
Wed, 04/08/2020 - 03:33
Because of the nutritional ecology of dung- and carrion-feeding, bacteria are the integral part of Lucilia sericata life cycle. Nevertheless, the disinfected larvae of the blowfly are applied to treat human chronic wounds in a biosurgery named maggot debridement therapy (MDT). To realize the effects of location/diet on the gut bacteria, to infer the role of bacteria in the blowfly ecology plus in...
Wed, 04/08/2020 - 03:33
Staphylococcus aureus is one of the main causative agents of food poisoning. This bacterium is an important component of cheese microbiota and plays an important role in foodborne diseases. Another important component of the microbiota is the lactic acid bacterium, which actively participates in processes that define the physicochemical, sensorial, and microbiological features of cheese. Of the...
Wed, 04/08/2020 - 03:33
The spread of cholera in the midst of an epidemic is largely driven by direct transmission from person to person, although it is well-recognized that Vibrio cholerae is also capable of growth and long-term survival in aquatic ecosystems. While prior studies have shown that aquatic reservoirs are important in the...
Wed, 04/08/2020 - 03:33
Hfq (host factor for phage Q beta) is key for posttranscriptional gene regulation in many bacteria. Hfq’s function is to stabilize sRNAs and to facilitate base-pairing with trans-encoded target mRNAs. Loss of Hfq typically results in pleiotropic phenotypes, and, in the major human pathogen Vibrio cholerae, Hfq inactivation has been...
Wed, 04/08/2020 - 03:33
During development, organisms acquire three-dimensional (3D) shapes with important physiological consequences. While basic mechanisms underlying morphogenesis are known in eukaryotes, it is often difficult to manipulate them in vivo. To circumvent this issue, here we present a study of developing Vibrio cholerae biofilms grown on agar substrates in which the...
Wed, 04/08/2020 - 03:33
The online e-pub version of the article entitled, Antibiotic Resistance in Salmonella Enteritidis Isolates Recovered from Chicken, Chicken Breast, and Humans Through National Antimicrobial Resistance Monitoring System Between 1996 and 2014” by Paudyal N, Pan H, Li X. Fang W. Yue M., Foodborne Pathog Dis [Epub ahead of print]; DOI: 10.1089/fpd.2017.2402 is being officially retracted from Foodborne...
Wed, 04/08/2020 - 03:33
This study evaluated the acid tolerance response (ATR) of two strains of Listeria monocytogenes (serotype 1/2a and 4b) and one strain of Listeria innocua under different mildly acid conditions. Cells were incubated in combinations of three concentrations of lactic acid medium (3, 4.75, and 15 mM) and three external pH's (pHex 5.0, 6.0, and 6.5), plus, a HCl control, and a blank control (pH 7.4)....
Wed, 04/08/2020 - 03:33
Necrotizing enterocolitis (NEC) is a serious inflammatory intestinal disorder with a high mortality rate, which occurs most commonly in newborn infants. Cronobacter sakazakii, a common contaminant in infant formula, is associated with NEC. However, its role in NEC pathogenesis is unknown, and there are still no effective treatments for NEC. Currently, natural bioactive products have been...
Wed, 04/08/2020 - 03:32
Author(s): Tania Majumdar , Runu Chakraborty , Utpal Raychaudhuri In this article, an amperometric immunosensor based on antibody immobilization onto the platinum (Pt) electrode surface by cross-linkage via glutaraldehyde (GA) pre-coated with polyethyleneimine (PEI) layer for the detection of Staphylococcus aureus (S. aureus) in food samples has been developed. Immobilization of antibodies on the...
Wed, 04/08/2020 - 03:32
This study evaluated the efficacy of lactic acid bacteria (LAB) isolated from traditional fermented foods and beverage of Ladakh as biocontrol agents against Yersinia enterocolitica. The antagonistic activities of 46 LAB isolates were screened for their ability to inhibit the growth of Y. enterocolitica at refrigeration temperatures using agar spot tests. On the basis of the 16S rDNA sequences,...
Wed, 04/08/2020 - 03:32
This study evaluated the efficacy of lactic acid bacteria (LAB) isolated from traditional fermented foods and beverage of Ladakh as biocontrol agents against Yersinia enterocolitica. The antagonistic activities of 46 LAB isolates were screened for their ability to inhibit the growth of Y. enterocolitica at refrigeration temperatures using agar spot tests. On the basis of the 16S rDNA sequences,...
Wed, 04/08/2020 - 03:32
Author(s): S.M. Castro, M. Kolomeytseva, R. Casquete, J. Silva, R. Queirós, J.A. Saraiva, P. Teixeira Mild pressure (300MPa, 5min, 10°C) was combined with lactic acid bacteria (Pediococcus acidilactici, HA-6111-2) and its bacteriocin (bacHA-6111-2) to treat traditional Portuguese ready-to-eat meat sausage (Chouriço de carne) and the effect on the survival of Listeria innocua was evaluated. Five...
Wed, 04/08/2020 - 03:32
This study investigated the effects of lactic acid bacteria (LAB) of goat's milk origin on growth and biofilm formation of two pathogenic strains viz. Pseudomonas aeruginosa and Staphylococcus aureus. Different LAB preparations such as untreated cell free supernatants (CFSs), pH neutralized CFS (N-CFS) and heat treated CFS (H-CFS) of lactobacilli were screened for their antimicrobial and biofilm...
Wed, 04/08/2020 - 03:32
Author(s): Gunaranjan Paturi, Christine A. Butts, Duncan Hedderley, Halina Stoklosinski, Sheridan Martell, Hannah Dinnan, Elizabeth A. Carpenter The influence of goat and cow milk powders individually and in combination with prebiotics (inulin and oligofructose) on gut microbial populations, organic acid concentrations and gut morphology were studied in newly weaned rats fed for 21 days. The...
Wed, 04/08/2020 - 03:32
Author(s): Gunaranjan Paturi, Christine A. Butts, Duncan Hedderley, Halina Stoklosinski, Sheridan Martell, Hannah Dinnan, Elizabeth A. Carpenter The influence of goat and cow milk powders individually and in combination with prebiotics (inulin and oligofructose) on gut microbial populations, organic acid concentrations and gut morphology were studied in newly weaned rats fed for 21 days. The...
Wed, 04/08/2020 - 03:32
Author(s): Gunaranjan Paturi, Christine A. Butts, Duncan Hedderley, Halina Stoklosinski, Sheridan Martell, Hannah Dinnan, Elizabeth A. Carpenter The influence of goat and cow milk powders individually and in combination with prebiotics (inulin and oligofructose) on gut microbial populations, organic acid concentrations and gut morphology were studied in newly weaned rats fed for 21 days. The...
Wed, 04/08/2020 - 03:32
Author(s): Sanjeev Kumar, Satyendra Gautam Sprouts are consumed worldwide as a highly nutritive food. However, the sprouting process is quite supportive of microbial and pathogenic growth. This makes the sprouts such as mung (Vigna radiata), lucerne (Medicago sativa) and chickpea (Cicer arietinum), potential sources of foodborne infections and intoxications. Their microbiological analyses showed...
Wed, 04/08/2020 - 03:32
Author(s): Anand Prakash, Vellingiri Vadivel, Durairajan Rubini, Paramasivam Nithyanand Linalool is the active constituent of essential oils of several plant species like coriander, rosewood and cinnamon, which is known to have strong antibacterial activity. However, its application in food systems is limited because of low water solubility, high volatility and low stability. The present study...
Wed, 04/08/2020 - 03:32
Author(s): Seoung Hyuk Kim, Wang June Kim, Seok-Seong Kang The inhibitory effect of Lactobacillus brevis DF01 and Pediococcus acidilactici K10, which had been isolated from kimchi, on enteropathogenic bacterial adhesion to intestinal epithelial cells as well as their probiotic potential was studied. After the incubation of Lb. brevis DF01 and P. acidilactici K10 in simulated gastrointestinal...
Tue, 04/07/2020 - 04:02
Liposome encapsulated citral was prepared by means of a hot homogenization method. The microstructure, and particle size and zeta potential of the capsules were analyzed by transmission electron microscope (TEM) and dynamic light scattering (DLS) respectively, in which the results showed a good dispersion stability of the citral-loaded liposome. In vitro tests showed that liposome encapsulated...
Tue, 04/07/2020 - 04:01
Listeria monocytogenes is a model facultative intracellular pathogen. Tight regulation of virulence proteins is essential for a successful infection, and the gene encoding the annotated thioredoxin YjbH was identified in two forward genetic screens as required for virulence factor production. Accordingly, an L. monocytogenes strain lacking yjbH is attenuated in a murine model of infection....
Tue, 04/07/2020 - 04:00
Author(s): Waode Munaeni, Widanarni, Munti Yuhana, Mia Setiawati, Aris Tri Wahyudi This study investigated the influence of Eleutherine bulbosa (Mill.) Urb. on the immune responses, bacterial population in the intestines, and resistance of white shrimp, Litopenaeus vannamei, against infection with Vibrio parahaemolyticus. Shrimp were fed with three dosages of powder, at 6.25 g kg–1 (P6.25), 12.5 ...
Tue, 04/07/2020 - 03:53
Fresh‐cut apples were dip‐coated in antimicrobial/antibrowning solutions. The developed solutions had the dual effectiveness of both controlling browning and reducing pathogenic bacterial populations, which maintained the fresh‐like appearance and texture of apple slices for 35 days and reduced populations of Salmonella to undetectable levels (<1 log) without growth throughout the storage at 4...
Tue, 04/07/2020 - 03:50
The second messenger cyclic di‐AMP (c‐di‐AMP) is essential for growth of many bacteria because it controls osmolyte homeostasis. c‐di‐AMP can regulate the synthesis of potassium uptake systems in some bacteria and also directly inhibits and activates potassium import and export systems, respectively. Therefore, c‐di‐AMP production and degradation have to be tightly regulated depending on the...
Tue, 04/07/2020 - 03:49
Pepper bacterial wilt is caused by the bacterial pathogen, Ralstonia solanacearum. It is the most destructive disease of many Solanaceous crops such as potatoes, tobacco, pepper, tomatoes, and eggplant and is a significant source of crop loss worldwide. Physical, cultural and chemical controls have been employed to combat this destructive disease. However, none of these strategies has been able...
Tue, 04/07/2020 - 03:45
Vibrio parahaemolyticus, a halophilic bacterium often found in the marine or estuarine environment is a well-known enteropathogen responsible for foodborne outbreaks associated with seafood. The pathogenic strains of V. parahaemolyticus are marked by the presence of thermostable direct hemoylsin (tdh) and/or TDH related hemolysin (trh) genes. This study aimed to investigate the prevalence and...
Tue, 04/07/2020 - 03:45
Salmonella enterica is the most frequently reported cause of foodborne illness. As in other microorganisms, chemotaxis affords key physiological benefits, including enhanced access to growth substrates, but also plays an important role in infection and disease. Chemoreceptor signaling core complexes, consisting of CheA, CheW and methyl-accepting chemotaxis proteins (MCPs), modulate the switching...
Mon, 04/06/2020 - 03:32
Author(s): Xinrui Lv, Li Wang, Jingfeng Zhang, Haiyan Zeng, Xun Chen, Lei Shi, Hualing Cui, Xiaoxin He, Lichao Zhao Accurate and rapid identification of the live fraction of pathogenic bacteria in beef food samples has emerged as a pressing issue due to the serious threat posed by pathogens to human health. Escherichia coli O157: H7, one of the most common pathogens in beef, causes hemorrhagic...
Mon, 04/06/2020 - 03:29
The objective of this study was to evaluate the antibacterial effect of Chinese wild blueberry extract and its fractions against Listeria monocytogenes, Staphylococcus aureus, Salmonella Enteritidis, and Vibrio parahaemolyticus. Chinese wild blueberry (Vaccinium uliginosum) crude extract (BBE) was obtained using methanol extraction, and sugars plus organic acids (F1), phenolics fraction (F2), and...
Mon, 04/06/2020 - 03:28
This study was aimed to evaluate the efficiency of the organic chlorine and acetic acid solutions on the inactivation of adhered cells of Escherichia coli, Cronobacter sakazakii and Klebsiella pneumoniae to lettuce. Besides, the growth and inactivation of K. pneumoniae adhered to lettuce was modeled. According to the findings, the use of chlorine solution (170 mg/ml of total residual chlorine)...
Mon, 04/06/2020 - 03:28
Viable but nonculturable (VBNC) cells were able to recover at 25°C, but their recovery extent was affected by the types of prestressed treatment and strain. Cold‐stressed, and tetracycline‐resistant Vibrio cells were more rapidly converted to VBNC state than nonstressed cells. V. cholerae has a higher risk than V. parahaemolyticus and V. vulnificus in frozen seafood. Abstract We compared the fate...
Sun, 04/05/2020 - 03:32
Author(s): Craig Baker-Austin, Claire Jenkins, Jerome Dadzie, Orson Mestanza, Erick Delgado, Andy Powell, Tim Bean, Jaime Martinez-Urtaza Globally, V. parahaemolyticus is the most prevalent food-poisoning bacterium associated with seafood consumption. To date, the epidemiology of Vibrio parahaemolyticus infections in the UK has remained unexplored. Here we analysed archived V. parahaemolyticus...
Sun, 04/05/2020 - 03:32
Author(s): Sihan Chen, Xinyan Yang, Shiqian Fu, Xue Qin, Tao Yang, Chaoxin Man, Yujun Jiang A sensitive and accurate strategy was presented based on gold nanoparticles (AuNPs) for visual detecting viable Salmonella typhimurium (S. typhimurium). In this paper, dual aptamers were used to capture S. typhimurium and PCR amplification, respectively. The highly stable network structure formed by the...

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