Bacterial pathogens

Tue, 10/15/2019 - 03:49
Escherichia coli O157:H7 is a foodborne pathogen, implicated in various multi-state outbreaks. It encodes Shiga toxin on a prophage, and Shiga toxin production is linked to phage induction. An E. coli strain, designated 0.1229, was identified that amplified Stx2a production when co-cultured with E. coli O157:H7 strain PA2. Growth of PA2 in 0.1229 cell-free supernatants had a similar effect, even...
Tue, 10/15/2019 - 03:47
Author(s): Katesaraporn Nuankeaw, Boonyanoot Chaiyosang, Thanapat Suebrasri, Somdej Kanokmedhakul, Saisaamorn Lumyong, Sophon BoonlueAbstractBioactive compounds of endophytic fungus Trichoderma polyalthiae were extracted from culture broth media. The crude extracts showed strong antimicrobial activity against human pathogens. Biologically active compounds were isolated and purified by...
Tue, 10/15/2019 - 03:47
Author(s): Elumalai Preetham, Abdul Salam Rubeena, Baskaralingam Vaseeharan, Mukesh Kumar Chaurasia, Jesu Arockiaraj, Rolf Erik OlsenAbstractThe study is carried out to understand the antimicrobial and immunological response of a potential immune molecule lectin, MmLec isolated from haemolymph of Speckled shrimp, Metapenaeus monoceros. MmLec was purified using mannose coupled Sepharose CL-4B...
Tue, 10/15/2019 - 03:37
Aims This study was aimed at revealing the composition of microbiota in leaves, roots and rhizosphere soil of wheat plants that are resistant or susceptible to stripe rust, one of the most widely destructive leaf diseases in wheat production. Methods and Results A total of 36 wheat plants that showed resistant or susceptible reactions to stripe rust were sampled. Three compartments of each plant...
Tue, 10/15/2019 - 03:34
Background In urban Australia, the burden of shigellosis is either in returning travelers from shigellosis-endemic regions or in men who have sex with men (MSM). Here, we combine genomic data with comprehensive epidemiological data on sexual exposure and travel to describe the spread of multidrug-resistant Shigella lineages. Methods A population-level study of all cultured Shigella isolates in...
Tue, 10/15/2019 - 03:34
Tuberculosis is the primary infectious disease killer worldwide, with a growing threat from multidrug-resistant cases. Unfortunately, classic growth-based phenotypic drug susceptibility testing (DST) remains difficult, costly, and time consuming, while current rapid molecular testing options are limited by the diversity of antimicrobial-resistant genotypes that can be detected at once. Next-...
Tue, 10/15/2019 - 03:34
Besides the major nutrients, phosphorus (P) is an essential mineral for both the host animal and the porcine gut microbiota. Different strategies including phytase supplementation and more recently lactic acid (LA) are used to enhance the P availability from cereals in pig diets; however, their impact on the gut microbiota has been rarely related to fecal shedding of opportunistic pathogens. The...
Tue, 10/15/2019 - 03:34
Both nosocomial pathogens, such as Streptococcus pneumoniae and Haemophilus influenzae and food-borne pathogens, such as Bacillus cereus and Clostridium perfringens are known to be detrimental to human and animal health. The effectiveness of currently used treatments for these pathogens becomes limited as resistant strains emerge. Therefore, new methods for eliminating bacterial pathogens must be...
Mon, 10/14/2019 - 03:29
The Salmonella enterica subsp. diarizonae serovar 61:k:1,5,(7) (SASd) has been found to be host-adapted to sheep, with a high prevalence in sheep herds worldwide. Infections are usually sub-clinical, however the ...
Sun, 10/13/2019 - 03:43
Author(s): Shijuan Shan, Rongrong Liu, Hanxiao Feng, Yonghuan Zhang, Fumiao Zhang, Cui Lv, Guiwen YangAbstractp65 is an important subunit of the transcription factor NF-κB in the regulation of immune response. In the present study, the p65 cDNA was identified from common carp (Cyprinus carpio L.) (named Ccp65). Phylogenetic analysis revealed that Ccp65 located in the same clade as piscine p65 and...
Sun, 10/13/2019 - 03:43
Author(s): Xiaogang Du, Dong Li, Yunkun Li, Jiayu Wu, Anqi Huang, Guixian Bu, Fengyan Meng, Fanli Kong, Xiaohan Cao, Xingfa Han, Xiaofu Pan, Wei fan, Shiyong Yang, Xianyin ZengAbstractMammal Toll-like receptor 5 (TLR5) can directly recognize bacterial flagellin and therefore to initiate the inflammatory signaling cascades and trigger body immune system to clear the “non-self” substances. In...
Sun, 10/13/2019 - 03:43
Author(s): Yu-Lei Chen, Keke Han, Xin Huang, Zhuoxing Zhang, Xihe Wan, Qian RenAbstractThe caspase is an essential module in the Drosophila immune deficiency (IMD) pathway, which plays a crucial role in countering pathogen infection. In this study, a gene named PcCaspase-3C was found in Procambarus clarkia with a full-length of 4684 bp, including a 1572 bp opening reading frame, which encoded a...
Sun, 10/13/2019 - 03:43
Author(s): Lingsheng Bao, Yuanhua Chen, Honghui Li, Jianshe Zhang, Ping Wu, Ke Ye, Honglian Ai, Wuying ChuAbstractGinkgo biloba leaf is widely used in traditional medicine in China. The present study aimed to illustrate the effects of dietary Ginkgo biloba leaf extract (GBLE) on growth performance and immune responses in common carp infected by Aeromonas hydrophila. Six different diets either not...
Sun, 10/13/2019 - 03:43
Author(s): Zhi-qiang Du, Bo Li, Xiu-li Shen, Kai Wang, Jie Du, Xiao-dong Yu, Jian-jun YuanAbstractThe main advantage of antimicrobial peptides (AMPs) used as the effectors in the innate immunity system of invertebrates is that the high specificity is not indispensable. And they play important roles in the systemic defenses against microbial invasion. In this study, a new full-length cDNA of the...
Sun, 10/13/2019 - 03:41
Author(s): Helena Taina Diniz-Silva, Larissa Ramalho Brandão, Mércia de Sousa Galvão, Marta Suely Madruga, Janeeyre Ferreira Maciel, Evandro Leite de Souza, Marciane MagnaniAbstractThe effects of the incorporation of the essential oils from Origanum vulgare L. (OVEO; 0.07 μL/g) and Rosmarinus officinalis L. (ROEO; 2.65 μL/g) in combination in Minas Frescal cheese on the counts of the probiotic...
Sun, 10/13/2019 - 03:31
Awareness of the consumer has increased the demand of safe and chemical-free foods, and consequently it has increased the demand of antibacterial bioactive compounds. In the present study, antibacterial compound produced by a local bacterial isolate NSD MTCC 10072, showing antagonistic activity against six human pathogens, was isolated, partially purified and characterized. Maximum production of...
Sun, 10/13/2019 - 03:31
Poultry vaccine programs are important for control of Salmonella infections. Although there are vaccines for Salmonella Enteritidis, Salmonella Typhimurium and Salmonella Typhi, there are no vaccines for Salmonella Infantis which has an increased rate in the world. In this study, it was aimed to generate aroA gene deleted mutant bacteria for the constitution of S. Infantis vaccine prototype and...
Sun, 10/13/2019 - 03:31
This study aims to describe the content of polymeric matrix components under different incubation temperatures and pH levels. Optimal biofilm production of 15 S. Virchow isolates occurred following the incubation in LB−NaCl for 72 h, at pH 6.6 and 20 °C. The expression of csgA, csgD, adrA and bcsA genes at 20 °C, 25 °C and 30 °C in S. Virchow DMC18 was analyzed, and it was discovered that the...
Sun, 10/13/2019 - 03:31
This study was designed to compare the differentially expressed proteins between antibiotic-sensitive and antibiotic-resistant Salmonella Typhimurium, Klebsiella pneumonia, and Staphylococcus aureus. The susceptibilities of wild-type (WT), ciprofloxacin (CIP) and/or oxacillin (OXA)-induced, and clinically isolated resistant (CCARM) S. Typhimurium (STWT, STCIP, and STCCARM), K. pneumoniae (KPWT,...
Sat, 10/12/2019 - 03:42
by Francesca Marotta, Giuliano Garofolo, Lisa di Marcantonio, Gabriella Di Serafino, Diana Neri, Romina Romantini, Lorena Sacchini, Alessandra Alessiani, Guido Di Donato, Roberta Nuvoloni, Anna Janowicz, Elisabetta Di Giannatale Campylobacter jejuni, a common foodborne zoonotic pathogen, causes gastroenteritis worldwide and is increasingly resistant to antibiotics. We aimed to investigate the...
Sat, 10/12/2019 - 03:38
Author(s): Payman Khademi, Abdulghaffar Ownagh, Karim Mardani, Mohammad KhaliliAbstractThe present study was conducted to determine the prevalence of C. burnetii in raw milk samples collected from water buffalos and cattle in Northwest of Iran (West Azerbaijan Province). A total number of 840 milk samples were randomly collected from buffalos and cattle belonged to three different geographical...
Sat, 10/12/2019 - 03:38
Author(s): Marc. K. Kouam, Herman M.F Biekop, Bridget Katte, Alexis TeguiaAbstractSalmonella infections in poultry farms are overlooked in many African countries; yet these infections are mostly zoonotic with impact on both poultry industry and public health. Considering the impact of Salmonella in laying hens, and the role of laying hens as a source of Salmonella outbreak in human, knowledge of...
Sat, 10/12/2019 - 03:38
Author(s): Bárbara Martín-Maldonado, Laura Montoro-Dasi, Maria Teresa Pérez-Gracia, Jaume Jordá, Santiago Vega, Francisco Marco-Jiménez, Clara MarinAbstractWild birds have repeatedly been found to be involved in the dissemination of enteric bacterial pathogens in the environment. The aim of this study was to determine the occurrence of Salmonella and Campylobacter as well as the antimicrobial...
Sat, 10/12/2019 - 03:34
Escherichia coli O157:H7 is an important foodborne pathogen and has been implicated in numerous food poisoning outbreaks worldwide. Although several microbiological and molecular methods have been developed to detect E. coli O157:H7, the difficulty to rapidly detect low levels of the foodborne bacteria persists. Here, the optimization of a filtration technique to concentrate and rapidly detect E...
Sat, 10/12/2019 - 03:33
This study dealt about the volatile profile of Brassica rapa subsp. pekinensis (BRARP) using gas chromatography‐mass spectrometry (GC‐MS) and computer‐based molecular docking method. Its antimicrobial effect was also evaluated to determine the bioactive potential of BRARP (1.5% and 2% w/v) extract on the shelf life of beef for 16 days of refrigeration storage. The Caenorhabditis elegans was used...
Sat, 10/12/2019 - 03:32
The indiscriminate use of antimicrobials in aquaculture results in antibiotic selection pressure and proliferation of antimicrobial resistant (AMR) bacteria. Frequent assessment of antimicrobial resistance in aquaculture environment is inevitable so as to reduce the passage of clinically important AMR from aquatic to other environment. The present study analysed the antimicrobial resistance of...
Sat, 10/12/2019 - 03:32
The present study aimed to investigate the incidence, virulence and antibiotic properties in Vibrio spp. isolated from cockles (Tegillarca granosa) marketed in Korea. A total of 32 Vibrio spp. isolates including V. parahaemolyticus (n=4), V. alginolyticus (n=11), V. diabolicus (n=14), and V. harveyi (n=3) were detected using gyrB sequencing. The phenotypic pathogenicity revealed DNase, amylase...
Sat, 10/12/2019 - 03:31
Aim To evaluate antimicrobial effect of paeoniflorin against carbapenem‐resistant Klebsiella pneumoniae (CRKP). Methods and Results Minimum inhibitory concentration (MIC) of paeoniflorin against CRKP was determined by agar dilution method. Changes in intracellular ATP concentration, intracellular pH (pHin), cell membrane potential and membrane integrity were investigated to assess the influence...
Sat, 10/12/2019 - 03:31
Background Despite the growing importance of probiotics vivid health benefits, an impediment to the use of new probiotic cultures is their safety. Hence, there is a need to strictly examine the biosafety as well as health benefits of probiotics in vivo model system. Results In this study, two lactic acid bacterial (LAB) cultures Lactobacillus fermentum NCMR 2826 and FIX proven for their in vitro...
Sat, 10/12/2019 - 03:29
Chronic prostatitis/chronic pelvic pain syndrome (CP/ CPPS) has a negative impact on the quality of life, and its etiology still remains unknown. Although many treatment protocols have been evaluated in CP/CPPS, the outcomes have usually been disappointing. Botulinum neurotoxin A (BoNT-A), produced from Clostridium botulinum, has been widely used to lower urinary tract dysfunctions such as...
Sat, 10/12/2019 - 03:29
The detection of catalytically active botulinum neurotoxins (BoNTs) can be achieved by monitoring the enzymatic cleavage of soluble NSF (N-ethylmaleimide-sensitive-factor) attachment protein receptor (SNARE) proteins by the toxins’ light chains (LC) in cleavage-based assays. Thus, for sensitive BoNT detection, optimal cleavage conditions for the clinically relevant A–F...
Fri, 10/11/2019 - 03:41
Bacteriophages that infect the foodborne pathogen Listeria monocytogenes were previously isolated from New York dairy farms. The complete genome sequences for three of these Listeria phages, with genome sizes of 64.6 to 65.7 kb, are presented here. Listeria phages LP-010, LP-013, and LP-031-2 are siphoviruses that belong to the genus Homburgvirus.
Fri, 10/11/2019 - 03:41
We report high-quality closed reference genomes for 1 bovine strain and 10 human Shiga toxin (Stx)-producing Escherichia coli (STEC) strains from serogroups O26, O45, O91, O103, O104, O111, O113, O121, O145, and O157. We also report draft assemblies, with standardized metadata, for 360 STEC strains isolated from watersheds, animals, farms, food, and human infections.
Fri, 10/11/2019 - 03:36
LWT
Author(s): Yuanyuan Pan, Yan Zhang, Jun-Hu Cheng, Da-Wen SunAbstractEffects of ultrasound pretreatments and dielectric barrier discharge (DBD) cold plasma on the inactivation of Listeria monocytogenes (L. monocytogenes) under various growth temperatures were investigated, and membrane fatty acid profiles of L. monocytogenes were also determined. Fatty acids anteiso-C15:0, anteiso-C17:0 and C15:0...
Fri, 10/11/2019 - 03:32
The present study assessed the occurrence, virulence determinants and antimicrobial susceptibility patterns of Vibrio spp. isolated from live Manila clam (Ruditapes philippinarum). A total of 31 Vibrio spp. including 27 V. diabolicus, 2 V. fluvialis, 1 V. alginolyticus, and 1 V. antiquarius were isolated and identified. Phenotypic detection of DNase, lipase, phospholipase, amylase, and caseinase...
Fri, 10/11/2019 - 03:30
EchAMP, the tenth most abundant transcript expressed in the mammary gland of echidna, has in vitro broad-spectrum antibacterial effects. However, the effects of EchAMP on mastitis, a condition where inflammation is triggered following mammary gland infection, has not been investigated. To investigate the impact of EchAMP against mastitis, EchAMP transgenic mice were generated. In antibacterial...
Fri, 10/11/2019 - 03:29
Campylobacter jejuni is the most common bacterial cause of foodborne zoonosis in the European Union. Infections are often linked to the consumption and handling of poultry meat. The aim of the present study was to investigate the caecal microbiota of birds infected with C. jejuni at different ages. Therefore, a total of 180 birds of the laying hybrid Lohmann Brown-Classic were housed in 12...
Fri, 10/11/2019 - 03:29
Necrotic enteritis (NE) causes huge economic losses to the poultry industry. Probiotics are used as potential alternatives to antibiotics to prevent NE. It is known that Clostridium butyricum can act as a probiotic that can prevent infection. However, whether or not it exerts a beneficial effect on NE in chickens remains elusive. Therefore, we investigated the impact of C. butyricum on immune...
Thu, 10/10/2019 - 03:41
ABSTRACT Nonmenstrual toxic shock syndrome (nmTSS), linked to TSST-1-producing CC30 Staphylococcus aureus, is the leading manifestation of toxic shock syndrome (TSS). Due to case rarity and a lack of tractable animal models, TSS pathogenesis is poorly understood. We developed an S. aureus abscess model in HLA class II transgenic mice to investigate pathogenesis and treatment. TSST-1 sensitivity...
Thu, 10/10/2019 - 03:40
Author(s): Stefan EBMEYER, Erik KRISTIANSSON, D.G. Joakim LARSSONAbstractObjectiveUnderstanding the origins of antibiotic resistance genes is important to assess and manage risks associated with the emergence of novel resistance genes. The chromosomal ampC gene (CAV-1) of Aeromonas caviae has been reported as the origin of mobile FOX cephalosporinases. The recent identification of A. caviae as...
Thu, 10/10/2019 - 03:32
Significance and Impact of the Study: Microbial contamination of air and surfaces in hospitals play an important role in healthcare‐associated infections. The aim was the assessment of Klebsiella pneumoniae elimination using radiant catalytic ionization (RCI). K. pneumoniae are aetiological agent of nosocomial infections, such as: pneumonia, infections of urinary tract, blood, e.t.c. The strains...
Thu, 10/10/2019 - 03:29
Microcins are low-molecular-weight, ribosomally produced, highly stable, bacterial-inhibitory molecules involved in competitive, and amensalistic interactions between Enterobacteriaceae in the intestine. These interactions take place in a highly complex chemical landscape, the intestinal eco-active chemosphere, composed of chemical substances that positively or negatively influence bacterial...
Thu, 10/10/2019 - 03:29
Colistin is a last resort antibiotic used for the treatment of human infections associated with carbapenemase-producing Enterobacteriales. Here, we evaluated the occurrence of mcr-1 and -2 plasmid-mediated colistin resistance in colistin and/or carbapenem resistant human clinical Enterobacteriales and other gram-negative bacteria (n = 543) as well as third generation cephalosporin-resistant (3GCR...
Thu, 10/10/2019 - 03:29
The aim of this study was to quantify, identify, evaluate antimicrobial resistance, and characterize the virulence factors of enteropathogenic (EPEC), Shiga-toxigenic (STEC), and enterohemorrhagic (EHEC) Escherichia coli in raw milk (RM) and legal (LMFC) and illegal (IMFC) Minas Frescal cheeses in southern and northeast Brazil. Illegal cheeses are those made without official inspection service or...
Thu, 10/10/2019 - 03:29
Clostridium perfringens enterotoxin (CPE) contributes to diarrhea and an often-lethal enterotoxemia. CPE action starts when it binds to claudin receptors, forming a small complex (90 kDa). Six small complexes then oligomerize to create prepores, followed by insertion of beta-hairpins from CPE to form beta-barrel pores named CH-1 or CH-2. Of the ~27 members of the human claudin protein family,...
Thu, 10/10/2019 - 03:29
Infections due to multi-drug resistant bacteria are on the rise and there is an urgent need to develop new antibacterials. In this regard, a series of six functionally diverse new quinazolinone compounds were synthesized by a facile one-pot reaction of benzoic acid derivatives, trimethoxymethane and aniline derivatives. Three compounds of 3-aryl-8-methylquinazolin-4(3H)-one, and 3-aryl-6,7-...
Wed, 10/09/2019 - 03:40
by Jin Ling, Hong Wang, Gaomin Li, Zhen Feng, Yufei Song, Peng Wang, Hong Shao, Hu Zhou, Gang Chen Background Escherichia coli is currently unable to be reliably differentiated from Shigella species by routine matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis. In the present study, a reliable and rapid identification method was established for...

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