Bacterial pathogens

Fri, 12/06/2019 - 03:38
by Raies A. Mir, Robert G. Schaut, Heather K. Allen, Torey Looft, Crystal L. Loving, Indira T. Kudva, Vijay K. Sharma Vaccination-induced Escherichia coli O157:H7-specific immune responses have been shown to reduce E. coli O157:H7 shedding in cattle. Although E. coli O157:H7 colonization is correlated with perturbations in intestinal microbial diversity, it is not yet known whether vaccination...
Fri, 12/06/2019 - 03:38
Salmonella enterica is a major global foodborne pathogen that causes gastroenteritis and, in some cases, death. Salmonella serovar Anatum has been increasingly associated with foodborne salmonellosis outbreaks. In this report, we announce two complete genome sequences of Salmonella Anatum isolated from papaya fruit.
Fri, 12/06/2019 - 03:36
Author(s): ZAHRA H. MOHAMMAD, ELSA A. MURANO, ROSANA G. MOREIRA, ALEJANDRO CASTILLOAbstractSince two outbreaks of salmonellosis were linked to the consumption of almonds in 2001 and 2004, the study of pathogen inactivation kinetics in almonds has been encouraged, often by conducting inoculated challenge studies. The inoculation method could affect the results of such challenge studies, because of...
Fri, 12/06/2019 - 03:29
High prevalence of antimicrobial resistance (AMR) to drugs of critically/highly importance of human and veterinary medicine was observed among the recovered Escherichia coli, Salmonella spp., and Staphylococcus aureus, circulating over the different stages of poultry in India. Upward trend in AMR rates was observed in poultry‐retail shops than in poultry‐farms. Multi‐drug resistance was observed...
Fri, 12/06/2019 - 03:27
BACKGROUND The consumption of pesticides‐contaminated sour cherry as fruit or juice has become a major health concern, and thus, intensified the search for alternative processing technologies such as pulsed electric fields (PEF), ozone (O), and ultrasonication (US). The novelties of this experimental study for sour cherry juice were four‐fold: (1) to quantify the removal efficiency of the new...
Fri, 12/06/2019 - 03:26
ABSTRACT Microorganisms are intentionally added at different stages of the food and feed chain (food or feed additive, novel food or plant protection product) and are subjected to regulation and safety assessment by the European Food Safety Authority. Safety evaluation is based on application dossiers for market authorisation to the European Commission. The qualified presumption of safety (QPS)...
Fri, 12/06/2019 - 03:26
ABSTRACT This study was conducted to evaluate the safety and bacterial profile of Dhanaan (Ethiopian traditional fermented camel milk). The composition of the microbial community in Dhanaan samples was analysed by a metagenomic approach of 16S rRNA gene amplicon sequencing. Metagenomic profiling identified 87 different bacterial microorganisms (OTUs) in six samples analysed. Although the Dhanaan...
Fri, 12/06/2019 - 03:26
Aim: The purpose of this study was to examine the effectiveness of botulinum toxin A (BoNT-A) therapy combined with rehabilitation on motor function in post-stroke patients. Methods: The following sources up to December 31, 2018, were searched from inception for articles in English: Pubmed, Scopus, CINAHL, Embase, PsycINFO, and CENTRAL. Trials using injections of BoNT-A for upper and lower limb...
Thu, 12/05/2019 - 04:17
by Catalina Pardo-Roa, Geraldyne A. Salazar, Loreani Noguera, Francisco J. Salazar-Echegarai, Omar P. Vallejos, Isidora Suazo, Bárbara M. Schultz, Irenice Coronado-Arrazola, Alexis M. Kalergis, Susan M. Bueno Pathogenicity island excision is a phenomenon that occurs in several Salmonella enterica serovars and other members of the family Enterobacteriaceae. ROD21 is an excisable pathogenicity...
Thu, 12/05/2019 - 04:17
by Julian E. Grass, Sunkyung Kim, Jennifer Y. Huang, Stephanie M. Morrison, Andre E. McCullough, Christy Bennett, Cindy R. Friedman, Anna Bowen Gastrointestinal illnesses are the most frequently diagnosed conditions among returning U.S. travelers. Although most episodes of travelers’ diarrhea do not require antibiotic therapy, fluoroquinolones (a type of quinolone antibiotic) are recommended for...
Thu, 12/05/2019 - 04:12
Author(s): Elvira Frías, Yenea Iglesias, Avelino Alvarez-Ordóñez, Miguel Prieto, Montserrat González-Raurich, Mercedes LópezAbstractThe efficacy of Cold Atmospheric Pressure Plasma (CAPP) for the inactivation of Salmonella enterica serovar Enteritidis, Salmonella enterica serovar Typhimurium, Listeria monocytogenes and Escherichia coli O157:H7 on tofu was evaluated. The potential of using Plasma...
Thu, 12/05/2019 - 04:10
Author(s): Haijing Hu, Kun Jia, Huhu Wang, Xinglian Xu, Guanghong Zhou, Shuwen HeAbstractSalmonella is the leading cause of infectious disease outbreaks associated with meat products, residual meat particles on food-contact surfaces can influence the biofilm formation of microorganisms. The transcriptional landscape and expression of novel small non-coding RNAs (sRNAs) were investigated during...
Thu, 12/05/2019 - 04:08
Author(s): Yan Qi, Yingshu He, Larry R. Beuchat, Wei Zhang, Xiangyu DengAbstractThe common use of gloves in retail practices represents a potential route for cross contamination of foodborne pathogens in fresh-cut produce. Using fresh-cut cantaloupe as a food model, we investigated factors that may influence glove-mediated cross contamination by Listeria monocytogenes and developed mathematical...
Thu, 12/05/2019 - 04:01
LWT
Author(s): Jin-Young Han, Won-Jae Song, Joo Hyun Kang, Sea C. Min, Sangheum Eom, Eun Jeong Hong, Seungmin Ryu, Seongbong Kim, Sangwoo Cho, Dong-Hyun KangAbstractWe evaluated the effect of plasma-activated water (PAW) on the populations of foodborne pathogens and food-spoilage microorganisms on Korean rice cake. PAW was produced by treating distilled water with two atmospheric dielectric-barrier...
Thu, 12/05/2019 - 03:58
Background Human leptospirosis mainly affects people in close occupational contact with domestic livestock and their products in New Zealand. The disease has an unquantified impact on both human health and animal production in the country. This study aimed to estimate the burden of leptospirosis in terms of disability‐adjusted life years (DALYs) and cost associated with loss due to absence from...
Thu, 12/05/2019 - 03:47
Fluoroquinolones are used to treat serious bacterial infections, including those caused by Escherichia coli and Salmonella enterica. The emergence of plasmid-mediated quinolone resistance (PMQR) represent a new challenge to the successful treatment of Gram-negative infections. As part of a long-term strategy to generate a reference database of closed plasmids from antimicrobial resistant...
Thu, 12/05/2019 - 03:47
Salmonella Typhimurium, a bacterial pathogen with high metabolic plasticity, can adapt to different environmental conditions; these traits enhance its virulence by enabling bacterial survival. Neutrophils play important roles in the innate immune response, including the production of microbicidal reactive oxygen species (ROS). In addition, the myeloperoxidase in neutrophils catalyzes the...
Thu, 12/05/2019 - 03:47
Listeria monocytogenes is a highly fatal foodborne causative agent that has been implicated in numerous outbreaks and related deaths of listeriosis in the world. In this study, six L. monocytogenes isolated from ready-to-eat (RTE) meat products were analysed using Whole Genome Sequencing (WGS) to identify virulence and resistance genes, prophage sequences, PCR-serogroups, and sequence types (STs...
Wed, 12/04/2019 - 03:37
Author(s): Wilson Gómez Manrique, Mayra Araguaia Pereira Figueiredo, Ives Charlie-Silva, Marco Antonio de Andrade Belo, Cristina Corsi DibAbstractIn order to understand the pathophysiology of melanomcrophage centers (MMCs) formation during the tilapia defense response to bacterial infections, the present study evaluated the response, in terms of area, number and pigment constitution, of splenic...
Wed, 12/04/2019 - 03:36
Author(s): Lang Xie, Yixi Tao, Ronghua Wu, Qin Ye, Hao Xu, Yun LiAbstractIt is documented that tlx1, an orphan homeobox gene, plays critical roles in the regulation of early spleen developmental in mammalian species. However, there is no direct evidence supporting the functions of tlx1 in non-mammalian species, especially in fish. In this study, we demonstrated that tlx1 is expressed in the...
Wed, 12/04/2019 - 03:36
Author(s): Xiaolin Meng, Wenpan Hu, Shengkui Wu, Zhenxiang Zhu, Ronghua Lu, Guokun Yang, Chaobin Qin, Liping Yang, Guoxing NieAbstractThe Chinese yam peel (CYP) is a by-product of yam processing that is rich in various nutrients and a good source for feed additives. This study investigated the effects of CYP on the intestinal microbiota and gut defence barrier of the common carp (Cyprinus carpio...
Wed, 12/04/2019 - 03:36
Author(s): Bing-Chang Lee, Chun-Wei Hung, Cheng-Yung Lin, Chen-Han Shih, Huai-Jen TsaiAbstractTo develop an alternative to conventional antibiotics used in the aquaculture and livestock industries, we employed Bacillus subtilis, considered a biosafe microorganism, to express the degradable antimicrobial peptide lactoferricin. An expression plasmid pP43-6LFBII-GFP, in which reporter GFP cDNA was...
Wed, 12/04/2019 - 03:36
Author(s): Fengyu Yuan, Zilan Yang, Ting Tang, Song Xie, Fengsong LiuAbstractSmall heat shock proteins (sHSPs) are ATP-independent chaperones and involved into various physiological and stress processes. In the present study, a 28.6-kD sHSP coding gene, MnHSP28.6, was cloned and characterized from the oriental river prawn Macrobrachium nipponense. Tissue distribution analysis via qPCR and western...
Wed, 12/04/2019 - 03:36
Author(s): Do Hyeon Kim, Wan Il Cho, Seung Ju LeeAbstractA novel method was developed to estimate the fault probability of basic events in fault tree analysis (FTA) for microbial contamination. A simple food preparation process was used for the analysis. The process consisted of mixing and cutting. The fault probability (PF) was for the occasion at excess level of E. coli compared to a threshold...
Wed, 12/04/2019 - 03:33
Author(s): Raquel Montiel, Ángela Peirotén, Sagrario Ortiz, Daniel Bravo, Pilar Gaya, Joaquín V. Martínez-Suárez, Julio Tapiador, Manuel Nuñez, Margarita MedinaAbstractThe effect of Serrano and Iberian dry-cured ham processing and ripening on Listeria monocytogenes inactivation at the surface of whole hams was investigated. Salted hams were surface inoculated (6 log CFU) with a cocktail of 4 L....
Wed, 12/04/2019 - 03:32
LWT
Author(s): Tareq M. Osaili, Fayeza Hasan, Dinesh Kumar Dhanasekaran, Reyad S. Obaid, Anas A. Al-Nabulsi, Sowmya Rao, Hera Fatima, Mutamed Ayyash, Ioannis Savvaidis, Richard HolleyAbstractThe study aimed to determine D and z-values of Escherichia coli O157:H7 strains and Salmonella spp. in camel lean meat burger (CM) and camel meat burger with hump fat (CMF). Two E. coli O157:H7 strains, S....
Wed, 12/04/2019 - 03:28
Aim The present investigation was aimed at isolating and identifying bacterial strains from cured vanilla beans. Additionally, the study focused on evaluating bacterial processes pertaining to the aromatic compounds production (ACP). Methods and Results Three bacteria were isolated from Vanilla planifolia beans, previously subjected to the curing process. According to morphological, biochemical...
Wed, 12/04/2019 - 03:26
The emergence of transferable colistin resistance (mcr genes) poses a threat to the effective use of polymyxins, which are last resort drugs to treat infections by multidrug- and carbapenem-resistant Gram-negative bacteria. The aims of the study were to investigate the occurrence of colistin resistance, to determine possible resistance mechanisms and then characterize the mcr-positive Escherichia...
Wed, 12/04/2019 - 03:26
Vibrio parahaemolyticus is a naturally occurring bacterium in estuarine waters and is a major cause of seafood-borne illness. The bacterium has been consistently identified in Pacific Northwest waters and elevated illness rates of vibriosis in Washington State have raised concerns among growers, risk managers, and consumers of Pacific oysters (Crassostrea gigas). In order to better understand pre...
Wed, 12/04/2019 - 03:26
Due to its rapid lethal effect in the early development stage of shrimp, acute hepatopancreatic necrosis disease (AHPND) has been causing great economic losses, since its first outbreak in southeast China in 2...
Tue, 12/03/2019 - 04:14
Bacterial lipopolysaccharides are major components and contributors to the integrity of Gram-negative outer membranes. The more conserved lipid A-core part of this complex glycolipid is synthesized separately from the hypervariable O-antigenic polysaccharide (OPS) part and they are joined in the periplasm prior to translocation to the outer membrane. Three different biosynthesis strategies are...
Tue, 12/03/2019 - 04:11
Author(s): Renjiao Gao, Li Chen, Wei Zhang, Shuo Zhang, Jie Rao, Jun HuAbstractThis study aims to investigate the effects of replacing different proportions of fishmeal with Antarctic krill (AK) on the growth performance, body composition and nonspecific immunity index of red swamp crayfish Procambarus clarkia. AK was used to replace 0 (control), 25%, 50% and 100% of the fishmeal in the basic...
Tue, 12/03/2019 - 03:58
Our review provides a comprehensive treatise on the progress in understanding teixobactin chemistry, structure−activity relationships, and mechanisms of antibacterial activity. Teixobactin represents an exciting starting point for the development of new antibiotics that can be used to combat multidrug‐resistant bacterial (“superbug”) infections. Abstract The discovery of antibiotics has led to...
Tue, 12/03/2019 - 03:50
The feeding trial was conducted to evaluate the potentials of Clostridium butyricum in the diet of tilapia. Fish (~14 g) were fed with basal diet supplemented with 0 (Control), 0.5 (C‐1), 1 (C‐2), 2 (C‐3), 4 (C‐4) and 8 (C‐5) g/kg commercial probiotic‐containing C. butyricum (1.5 × 108 CFU/g) for 8 weeks. The results showed that weight gain significantly increased, and feed conversion ratio...
Tue, 12/03/2019 - 03:46
The intestine is the main reservoir of bacterial pathogens in most organisms. Klebsiella pneumoniae is an important opportunistic pathogen associated with nosocomial bacterial infections. Intestinal colonization with K. pneumoniae has been shown to be associated with an increased risk of subsequent infections. However, not all K. pneumoniae strains in the intestine cause further infection, and...
Tue, 12/03/2019 - 03:46
Protein secretion plays a crucial role for bacterial pathogens, exemplified by facultative human-pathogen Vibrio cholerae, which secretes various proteinaceous effectors at different stages of its lifecycle. Accordingly, the identification of factors impacting on protein secretion is important to understand the bacterial pathophysiology. PglLVc, a predicted oligosaccharyltransferase of V....
Tue, 12/03/2019 - 03:46
Extended-spectrum β-lactamases (ESBLs) form the most important resistance determinants prevalent worldwide. Data on ESBL-producing Escherichia coli from poultry and livestock are scarce in India. We present data on the functional and genomic characterization of ESBL-producing E. coli obtained from poultry in India. The whole genome sequences of 28 ESBL-producing E. coli were analyzed comprising...
Mon, 12/02/2019 - 03:36
Author(s): Gaoyang Wang, Yutong Lei, Ting Kang, Zhi Li, Hui Fei, Shun Yang, Peng Zhou, Chunsheng Wang, Zhengbing Lv, Mengmeng Huang, Xuewei XuAbstractC-type lectins are Ca2+-dependent carbohydrate-binding proteins containing one or more carbohydrate-recognition domains (CRDs). C-type lectins play crucial roles in innate immunity, including nonself-recognition and pathogen elimination. In the...
Mon, 12/02/2019 - 03:34
Author(s): Ivana Kubašová, Dzung B. Diep, Kirill V. Ovchinnikov, Andrea Lauková, Viola StrompfováAbstractOne of the relatively widespread properties of many enterococcal strains is the production of bacteriocins, which could be useful as natural food preservatives inhibiting pathogenic and spoilage microorganisms. However, knowledge of the distribution and spectrum of bacteriocin activity as well...
Mon, 12/02/2019 - 03:34
Author(s): Maged El-Ashker, Mayada Gwida, Stefan Monecke, Fatma El-Gohary, Ralf Ehricht, Mona Elsayed, Akinduti Paul, Mohamed El-Fateh, Sven MaurischatAbstractMethicillin-resistant S. aureus from cows with mastitis has received a growing interest worldwide. The present study aimed to provide a detailed description of the resistance and virulence traits of isolates from bovine mastitis samples.A...
Mon, 12/02/2019 - 03:34
Author(s): Mayada Gwida, Amal Awad, Maged El-Ashker, Helmut Hotzel, Stefan Monecke, Ralf Ehricht, Elke Müller, Annett Reißig, Stefanie A. Barth, Christian Berens, Sascha D. BraunAbstractThe objective of our study was to provide a molecular analysis using DNA-microarray based assays of commensal E. coli populations from apparently healthy livestock and their attendants to assess the virulence...
Mon, 12/02/2019 - 03:34
Author(s): Chang-Wei Lei, Yu Zhang, Zhuang-Zhuang Kang, Ling-Han Kong, Yi-Zhi Tang, An-Yun Zhang, Xin Yang, Hong-Ning WangAbstractHuman salmonellosis caused by the consumption of eggs and chicken meat contaminated with Salmonella Enteritidis has become a continuing public health concern worldwide. In this study we adopted whole genome sequencing (WGS) to determine the genetic relationship and...
Mon, 12/02/2019 - 03:32
LWT
Author(s): Mariana Cap, Paola Fleitas Paredes, Diego Fernández, Marina Mozgovoj, Sergio R. Vaudagna, Anabel RodriguezAbstractThe aim of this study was to evaluate the effect of the pressure level and holding time on the Salmonella spp inactivation during HHP processing in frozen chicken breast fillets. Once identified the most effective process, meat quality (color and texture) was evaluated....
Mon, 12/02/2019 - 03:32
LWT
Author(s): Andréia Saldanha de Lima, Darla Volcan Maia, Louise Haubert, Thaís Larré Oliveira, Ângela Maria Fiorentini, Cesar Valmor Rombaldi, Wladimir Padilha da SilvaAbstractAraçá (Psidium cattleianum Sabine) is a native fruit of South America, with high levels of bioactive compounds as well as antimicrobial potential. The aim of this study was to evaluate the antibacterial activity of the...
Mon, 12/02/2019 - 03:32
LWT
Author(s): Jasim Ahmed, Linu Thomas, Mehrajfatema MullaAbstractHummus is a popular dip of the Middle Eastern countries and widely sold in the supermarket. The consumption of hummus from these sources could be a potential source of microbial contamination and food-borne diseases. In this work, the hummus samples were subjected to high-pressure treatment (300–600 MPa for 15 min) in three selected...

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