Campylobacter

Tue, 11/19/2019 - 03:38
Campylobacter jejuni is a leading cause of foodborne illnesses worldwide. Its porA gene encodes the major outer membrane protein (MOMP) that is abundantly expressed and has important physiological functions, including a key role in systemic infection and abortion induction in pregnant animals. Despite the importance of porA in C. jejuni pathogenesis, mechanisms modulating its expression levels...
Fri, 11/15/2019 - 03:32
Author(s): Zifeng Han, Li Li, Thomas Willer, Wolfgang Baumgärtner, Silke RautenschleinAbstractCampylobacter jejuni (C. jejuni) is a predominant cause of foodborne illness in humans, while its colonization in chickens is usually asymptomatic. Antibiotics are not routinely used to treat chickens against C. jejuni, but in the face of other bacterial diseases, C. jejuni may be exposed to antibiotics...
Thu, 11/14/2019 - 03:36
ABSTRACT O-linked protein glycosylation is a conserved feature of the Burkholderia genus. The addition of the trisaccharide β-Gal-(1,3)-α-GalNAc-(1,3)-β-GalNAc to membrane exported proteins in Burkholderia cenocepacia is required for bacterial fitness and resistance to environmental stress. However, the underlying causes of the defects observed in the absence of glycosylation are...
Mon, 11/11/2019 - 03:26
A matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) method were established to detect Campylobacter jejuni and Listeria monocytogenes from a chicken farm and samples from commercial live chickens. Suspected isolates were pretreated and crystallized by matrix solutions consisting of ethanol, acetonitrile, and water with different proportions. The effect of...
Sun, 11/10/2019 - 03:26
Aims Current culture‐based methods for detection and determination of Campylobacter levels on processed chickens takes at least two days. Here, we sought to develop a new complete, low‐cost and rapid (approximately 2·5 hours) detection system requiring minimal operator input. Methods and Results We observed a strong correlation between culture‐based cell counts and our ability to detect either...
Fri, 11/08/2019 - 03:39
by Francesca Marotta, Giuliano Garofolo, Lisa Di Marcantonio, Gabriella Di Serafino, Diana Neri, Romina Romantini, Lorena Sacchini, Alessandra Alessiani, Guido Di Donato, Roberta Nuvoloni, Anna Janowicz, Elisabetta Di Giannatale
Thu, 11/07/2019 - 03:29
This study investigated the occurrence of shiga toxin‐producing Escherichia coli (STEC), thermotolerant Campylobacter spp. and Salmonella spp. in Swedish dairy milk. A total of 302 inline milk filters were analyzed. Salmonella was not isolated from any filters. Polymerase chain reaction screening detected thermotolerant Campylobacter in 30.5% of the milk filters analyzed and it was isolated from...
Wed, 11/06/2019 - 03:26
SUMMARY Microbiological safety and quality of fresh chicken meat are important concerns to industry. The objective of this study was to investigate the effects of in-package, non-thermal high voltage dielectric barrier discharge (HVDBD) treatment on microbial quality, safety, and color of fresh chicken breast meat (pectoralis major). Boneless skinless chicken breast meat was collected from a...
Wed, 11/06/2019 - 03:26
SUMMARY Salmonella and Campylobacter are the two pathogens commonly associated with raw poultry meat, as poultry products are frequent vehicles of these bacteria. The objective of the current research was to determine the optimal contact time for 6 antimicrobial treatments, including water, 0.003% chlorine, 0.07% peracetic acid (PAA), 0.1% PAA, 0.35% cetylpyridinium chloride (CPC), and 0.6% CPC...
Fri, 11/01/2019 - 03:34
Author(s): Zahra Dehghani, Javad Mohammadnejad, Morteza Hosseini, Bita bakhshi, Ali Hossein RezayanAbstractIn this work a new fluorescence immunosensor with use of graphene oxide and graphene quantum dot for detection Campylobacter jejuni whole cell in food samples was designed. This biosensor was designed based on interaction of poly clonal antibody conjugated with graphene quantum dot with...
Fri, 11/01/2019 - 03:33
Author(s): Kate M. Thomas, William A. de Glanville, Gary C. Barker, Jackie Benschop, Joram J. Buza, Sarah Cleaveland, Margaret A. Davis, Nigel P. French, Blandina T. Mmbaga, Gerard Prinsen, Emmanuel S. Swai, Ruth N. Zadoks, John A. CrumpAbstractBackgroundCampylobacter and Salmonella, particularly non-typhoidal Salmonella, are important bacterial enteric pathogens of humans which are often carried...
Thu, 10/31/2019 - 03:37
Campylobacter jejuni and Campylobacter coli are leading causes of human foodborne illness, with poultry as a major vehicle. Turkeys are frequently colonized with Campylobacter, but little is known about Campylobacter survival in turkey feces, even though fecal droppings are major vehicles for Campylobacter within-flock transmission as well as for environmental dissemination. Our objective was to...
Wed, 10/30/2019 - 03:26
Campylobacter jejuni (C. jejuni) is considered as an opportunistic zoonotic pathogen that may cause gastroenteritis in humans and other animals. Wild birds may be as potential vectors of C. jejuni around urban and suburban areas. Here, 520 samples were collected from 33 wild bird species in urban and suburban areas, Beijing. In total 57 C. jejuni were isolated from seven species. It was found...
Thu, 10/24/2019 - 03:33
Campylobacteriosis is one of the most common foodborne diseases worldwide. Two Campylobacter species – C. jejuni and C. coli in poultry and poultry products are considered to be the main source of human campylobacteriosis. Therefore, studying Campylobacter status in poultry flocks is needed to prevent transmission of disease and reduce human risk, health cost, and economic losses. In this study,...
Wed, 10/23/2019 - 03:50
Antimicrobial resistance (AMR) is a major public health problem that requires publicly available tools for rapid analysis. To identify AMR genes in whole-genome sequences, the National Center for Biotechnology Information (NCBI) has produced AMRFinder, a tool that identifies AMR genes using a high-quality curated AMR gene reference database. The Bacterial Antimicrobial Resistance Reference Gene...
Wed, 10/23/2019 - 03:46
Author(s): Stanley H. Chen, Narelle Fegan, Chawalit Kocharunchitt, John P. Bowman, Lesley L. DuffyAbstractUnderstanding the bacterial community profile through poultry processing could help the industry to produce better poultry products. In this study, 10 chicken carcasses were randomly sampled from before and after scalding, before and after immersion chilling, and after air chilling each...
Tue, 10/22/2019 - 03:33
Campylobacteriosis is the most commonly reported zoonosis in Ireland and European Union, posing a serious health burden with 9 million cases reported annually in the European Union. Chicken meat is recognized as a major source of human infections, with an estimated 20%–30% of cases of campylobacteriosis in the European Union attributed to the handling, preparation, and consumption of broilers....
Tue, 10/22/2019 - 03:28
To better understand public health implications of waterfowl as reservoirs for zoonotic sources of Campylobacter in recreational waters, we developed a Gallus gallus (chick) model of infection to assess the pathogenicity of environmental isolates of Campylobacter. This method involved exposure of 1-day-old chicks through ingestion of water, the natural route of infection. Viable Campylobacter...
Sun, 10/20/2019 - 03:41
Author(s): Cathrine F. Kure, Lars Axelsson, Mats Carlehög, Ingrid Måge, Merete R. Jensen, Askild HolckAbstractSalmonella and Campylobacter represent the two most commonly reported zoonoses in EU and cause a significant health burden. The effects of a prototype steam treatment system (Deconizer) on reduction of aerobic bacteria, Salmonella Enteritidis and Campylobacter jejuni on chicken carcasses...
Fri, 10/18/2019 - 03:29
Campylobacter jejuni (C. jejuni) are able to colonise and infect domestic poultry and also pose a risk for humans. The aim of this study was to determine the extent of genotypic diversity among C. jejuni isolates recovered from avian and human sources in Egypt. Furthermore, the short variable region (SVR) of flagellin A (flaA) gene was analysed for the presence of allelic variants. Our results...
Thu, 10/17/2019 - 03:31
The sugar glider (Petaurus breviceps) is a small, arboreal, nocturnal, gliding mammalian possum belonging to the marsupial infraclass. Exotic marsupials, including sugar gliders, are becoming popular companion pets and, consequently, the risk of potential infections that can be transmitted to humans should be investigated. Data on the role of the sugar glider as a possible carrier of pathogenic...
Sat, 10/12/2019 - 03:42
by Francesca Marotta, Giuliano Garofolo, Lisa di Marcantonio, Gabriella Di Serafino, Diana Neri, Romina Romantini, Lorena Sacchini, Alessandra Alessiani, Guido Di Donato, Roberta Nuvoloni, Anna Janowicz, Elisabetta Di Giannatale Campylobacter jejuni, a common foodborne zoonotic pathogen, causes gastroenteritis worldwide and is increasingly resistant to antibiotics. We aimed to investigate the...
Sat, 10/12/2019 - 03:38
Author(s): Bárbara Martín-Maldonado, Laura Montoro-Dasi, Maria Teresa Pérez-Gracia, Jaume Jordá, Santiago Vega, Francisco Marco-Jiménez, Clara MarinAbstractWild birds have repeatedly been found to be involved in the dissemination of enteric bacterial pathogens in the environment. The aim of this study was to determine the occurrence of Salmonella and Campylobacter as well as the antimicrobial...
Fri, 10/11/2019 - 03:29
Campylobacter jejuni is the most common bacterial cause of foodborne zoonosis in the European Union. Infections are often linked to the consumption and handling of poultry meat. The aim of the present study was to investigate the caecal microbiota of birds infected with C. jejuni at different ages. Therefore, a total of 180 birds of the laying hybrid Lohmann Brown-Classic were housed in 12...
Mon, 10/07/2019 - 03:38
Author(s): Logan Meurer, William Payne, J. Stephen GuffeyAbstractCampylobacter jejuni is a gram negative, microaerophilic bacteria that is the leading cause of food-borne illnesses in the United States, frequently producing severe diarrhea. Campylobacter jejuni has recently demonstrated antibiotic resistance. Alternative methods for treating these infections must be investigated. Visible light...
Sat, 10/05/2019 - 03:36
Author(s): Hossein Haghighi, Serge Kameni Leugoue, Frank Pfeifer, Heinz Wilhelm Siesler, Fabio Licciardello, Patrizia Fava, Andrea PulvirentiAbstractThe main aim of this study was to characterize microstructural, physical, optical, mechanical, water barrier and antimicrobial properties of chitosan-polyvinyl alcohol blend films (CS-PVA) enriched with ethyl lauroyl arginate (LAE) (1–10% w/v) for...
Wed, 10/02/2019 - 03:41
A viability quantitative PCR (qPCR) utilizing propidium monoazide (PMA) is presented for rapid quantification of viable cells using the foodborne pathogen Campylobacter coli as a bacterial model. It includes optimized spheroplast formation via lysozyme and EDTA, induction of a mild osmotic shock for enhancing the selective penetration of PMA into dead cells, and exploitation of an internal sample...
Tue, 10/01/2019 - 00:00
Campylobacter jejuni (C. jejuni), a foodborne pathogen, is a major contributor to human bacterial gastroenteritis worldwide and detrimental to public health. In this paper, we developed a dual-mode aptasensor for Campylobacter jejuni (C. jejuni) determination. Based on surface-enhanced Raman scattering (SERS) and chiral optical response, the concentrations of C. jejuni in...
Mon, 09/30/2019 - 16:54
ABSTRACT The stator units of the flagellum supply power to the flagellar motor via ion transport across the cytoplasmic membrane and generate torque on the rotor for rotation. Flagellar motors across bacterial species have evolved adaptations that impact and enhance stator function to meet the demands of each species, including producing stator units using different fuel types or various stator...
Sun, 09/29/2019 - 03:30
Author(s): Tanya Christidis, Matt Hurst, Wallis Rudnick, Katarina D.M. Pintar, Frank PollariAbstractSalmonella is one of the leading causes of gastro-enteric disease in Canada, second only to Campylobacter. The mean exposure of Salmonella measured in cells ingested per person per day, from 15 transmission routes, including foodborne, animal contact and waterborne routes, was estimated for Canada...
Sat, 09/28/2019 - 03:29
Author(s): Luigi Iannetti, Diana Neri, Gino Angelo Santarelli, Giuseppe Cotturone, Michele Podaliri Vulpiani, Romolo Salini, Salvatore Antoci, Gabriella Di Serafino, Elisabetta Di Giannatale, Francesco Pomilio, Stefano MessoriAbstractStress factors and poor animal welfare can increase the susceptibility of food-producing animals to diseases, posing microbial risks to consumers. Animal welfare...
Sat, 09/28/2019 - 03:24
Sixteen sites in the watershed of the South Fork of the Broad River (SFBR) in Northeastern Georgia, USA, were sampled in two seasons to detect Campylobacter. Sites were classified as mostly influenced by forest, pasture, wastewater pollution control plants (WPC) or mixed‐use. Sampling was repeated in the late spring and late fall for two years for a total of 126 samples. Free‐catch water and...
Wed, 09/25/2019 - 03:37
Clostridioides (Clostridium) difficile infection (CDI) is the most common causative pathogen of health care-associated gastrointestinal infections; however, due to the overlap of clinical symptoms with those of other causes of acute gastroenteritis, the selection of the most appropriate laboratory test is difficult. From April to October 2018, 640 stool samples requested for CDI testing were...
Fri, 09/13/2019 - 03:26
Background The ability to identify bacterial pathogens that necessitate specific clinical management or public health action in children with acute gastroenteritis is crucial to patient care and public health. However, existing stool-testing guidelines offer inconsistent recommendations, and their performance characteristics are unknown. We evaluated 6 leading gastroenteritis guidelines (eg,...
Thu, 09/12/2019 - 03:37
Author(s): Byron Michael Berenger, Christina Ferrato, Linda ChuiAbstractTransport media are recommended to improve the sensitivity of fecal culture, but there are limited published data comparing bacterial viability in feces stored with or without transport media. In this study, recovery of bacteria from culture-positive feces after 7 days of storage was assessed under the following conditions:...
Thu, 09/12/2019 - 03:27
ABSTRACT Campylobacter jejuni, a major cause of human gastroenteritis worldwide, is often associated with the consumption of contaminated poultry products. With increasing consumer preference to natural and minimally processed foods, interventions utilizing natural antimicrobials for controlling C. jejuni on poultry products are gaining popularity. This study investigated the efficacy of the...
Wed, 09/11/2019 - 03:22
The Campylobacter and Arcobacter genera encompass closely related species that are ubiquitous in nature and are harboured in the gastrointestinal tract of many animals, including food-producing animals (cattle, sheep, pigs and poultry). In humans Campylobacter spp. is the cause of most of the gastroenteritis cases worldwide and in more severe cases the infection can result in Guillian Barré...
Thu, 09/05/2019 - 20:00
Campylobacter jejuni is the leading bacterial cause of acute gastroenteritis worldwide and is of immense significance to public health. C. jejuni primarily lives in the gastrointestinal tracts of poultry and can contaminate meat during processing. Despite a small genome, the metabolic plasticity of C. jejuni allows proliferation in chicken ceca and mammalian host intestines, and survival in...
Thu, 09/05/2019 - 03:54
Campylobacter is among the four main causes of gastroenteritis worldwide and has increased in both developed and developing countries over the last 10 years. The vast majority of reported Campylobacter infections are caused by Campylobacter jejuni and, to a lesser extent, C. coli; however, the increasing recognition of other emerging Campylobacter pathogens is urgently demanding a better...
Thu, 09/05/2019 - 03:52
Author(s): Golnaz Divsalar, Hami Kaboosi, Rahem Khoshbakht, Hesamaddin Shirzad-Aski, Fatemeh Peyravii GhadikolaiiAbstractThe aims of this study were to regain new epidemiology information about frequency, drug resistance rates, and typing of Campylobacter jejuni (C. jejuni) isolates, obtained from some poultry and cattle farms, slaughterhouses, and people with diarrhea. In this regard, Minimal...
Thu, 09/05/2019 - 03:52
Author(s): Sobia Kanwal, Zobia Noreen, Vajeeha Aalam, Junaid Akhtar, Fariha Masood, Sundus Javed, Habib BokhariAbstractCampylobacter jejuni is a major cause of infectious diarrhea in humans. The bacterium can be transmitted through contaminated poultry meat and waste water. We report the presence of C. jejuni from potential transmission sources including egg shells, poultry waste, waste water and...
Thu, 09/05/2019 - 03:50
In 2015, a novel thermophilic Campylobacter was isolated from cases of spotty liver disease in laying hens in the UK. In 2016, it was isolated from laying hens in Australia where it was formally named Campylobacter hepaticus and confirmed as the cause of spotty liver disease. It has also been isolated from laying hens in America. It is fastidious, grows slowly on first isolation and does not grow...
Wed, 09/04/2019 - 03:56
The gut microbiome is critical in providing resistance against colonization by exogenous microorganisms. The mechanisms via which the gut microbiota provide colonization resistance (CR) have not been fully elucidated, but they include secretion of antimicrobial products, nutrient competition, support of gut barrier integrity, and bacteriophage deployment. However, bacterial enteric infections are...
Thu, 08/29/2019 - 15:34
Abstract The efficacy of the natural plant‐derived compound, eugenol (EG), as an antimicrobial wash treatment to reduce Campylobacter jejuni in postharvest poultry was investigated. The antimicrobial efficacy of EG was studied as a suspension, emulsion, or nanoemulsion treatment (two trials each). In each trial, chicken skin samples were inoculated with C. jejuni (∼7.2 Log CFU/sample), washed...
Tue, 08/06/2019 - 20:00
Campylobacter jejuni is a Gram-negative rod-shaped bacterium that commensally inhabits the intestinal tracts of livestock and birds, and which also persists in surface waters. C. jejuni is a leading cause of foodborne gastroenteritis, and these infections are sometimes associated with the development of post-infection sequelae such as Guillain-Barré Syndrome. Flagella are considered a primary...
Tue, 08/06/2019 - 20:00
Campylobacter jejuni is the leading cause of human foodborne illness globally, and is strongly linked with the consumption of contaminated poultry products. Several studies have shown that C. jejuni can form sanitizer tolerant biofilm leading to product contamination, however, limited research has been conducted to develop effective control strategies against C. jejuni biofilms. This study...

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