Campylobacter

Sat, 01/16/2021 - 10:03
Significant knowledge gaps exist in our understanding of Campylobacter jejuni contamination of the poultry production continuum. Microbiological surveillance and genotypic characterization were undertaken on C. jejuni isolates longitudinally recovered from three poultry farms (weekly samples), the abattoir at which birds were processed, and at retail over a 542-day period in southwestern Alberta...
Tue, 01/12/2021 - 04:02
Campylobacter spp. and Arcobacter butzleri are foodborne pathogens associated with the consumption of contaminated raw chicken meat. At the industry level, the combination of new and common antimicrobials could be used as an strategy to control the presence of pathogens in chicken carcasses. The objective of this study was to determine the bacteriostatic and bactericidal effect of a mixture of...
Mon, 01/11/2021 - 22:14
The aim of this work was the establishment of a national laboratory sentinel surveillance service for human clinical Campylobacter in Ireland. This included detailed genomic molecular epidemiology of Campylobacter for 2019. For February‐December 2019, 24 clinical microbiology laboratories in Ireland submitted all PCR/culture‐positive clinical Campylobacter spp. specimens to Public Health...
Mon, 01/11/2021 - 16:00
Campylobacter jejuni CsrA is an mRNA-binding, post-transcriptional regulator that controls many metabolic- and virulence-related characteristics of this important pathogen. In contrast to E. coli CsrA, whose activity is modulated by binding to small non-coding RNAs (sRNAs), C. jejuni CsrA activity is controlled by binding to the CsrA antagonist FliW. In this study, we identified the FliW binding...
Fri, 01/08/2021 - 16:00
Campylobacter jejuni (CJ) is the most prevalent zoonotic pathogen of chicken meat and related products, which may lead to gastroenteritis and autoimmune diseases in humans. Although controlling this bacterium is important, CJ strains resistance against traditional antibiotic therapy has been increased. Vegetable oils and fats are natural biomaterials explored since the Ancient times, due to their...
Fri, 01/08/2021 - 16:00
Campylobacter jejuni is the leading cause of bacterial gastroenteritis, which has motivated the monitoring of genetic profiles circulating in Luxembourg since 13 years. From our integrated surveillance using a genotyping strategy based on an extended MLST scheme including gyrA and porA markers, an unexpected endemic pattern was discovered in the temporal distribution of genotypes. We aimed to...
Wed, 01/06/2021 - 06:07
Author(s): Zahra Nooreh, Kamran Taherpour, Mohammad Akbari Gharaei, Hassan Shirzadi, Hossein Ali Ghasemi Colonization of the gastrointestinal tract by potentially pathogenic bacteria and their shedding in animal feces is a fundamental factor for both animal health and human food safety. This study was conducted to evaluate the efficacy of salinomycin, direct-fed microbial, and Ferulago angulata...
Wed, 01/06/2021 - 06:07
Author(s): Joshua M. Lyte, Sandip Shrestha, Basanta R. Wagle, Rohana Liyanage, Diego A. Martinez, Annie M. Donoghue, Karrie M. Daniels, Mark Lyte Microbial endocrinology, which is the study of neurochemical-based host-microbe interaction, has demonstrated that neurochemicals affect bacterial pathogenicity. A variety of neurochemicals, including norepinephrine, were shown to enhance intestinal...
Tue, 01/05/2021 - 10:03
ABSTRACT The substantial rise in multidrug-resistant bacterial infections is a current global imperative. Cumulative efforts to characterize antimicrobial resistance in bacteria has demonstrated the spread of six families of multidrug efflux pumps, of which resistance-nodulation-cell division (RND) is the major mechanism of multidrug resistance in Gram-negative bacteria. RND is composed of a...
Mon, 01/04/2021 - 22:13
In the present study, changes in the quality characteristics of the albumin and yolk parts of the eggs obtained from different poultry systems for four seasons were investigated. The highest egg weight, shell thickness, dry shell weight, shell deformation, shell fracture and vitellin membrane strength values were determined to be in cage systems with 70.92g, 0.45 mm, 9.46g, 0.79 mm, 71.80 N and 0...
Mon, 01/04/2021 - 16:00
Campylobacter jejuni is the leading cause of bacterial gastroenteritis worldwide and the main source of infection is contaminated chicken meat. Although this important human pathogen is an obligate microaerophile...
Fri, 01/01/2021 - 14:05
Campylobacter jejuni is among the leading causes of bacterial foodborne illness. Poultry is the major reservoir and source of human campylobacteriosis. Currently, there is no effective and practical method to decrease C. jejuni colonization in chickens or to reduce human infections. Additionally, antibiotic-resistant infections pose a serious public health concern; therefore, antibiotic-...
Fri, 12/25/2020 - 06:10
Author(s): Masaru Usui, Sho Tateno, Masanobu Onozaki, Naoaki Misawa, Yasuhiko Suzuki, Yutaka Tamura, Chie Nakajima A rapid method for the simultaneous detection of fluoroquinolone- and macrolide-resistant Campylobacter jejuni/coli in food products is long-awaited. In this study, we developed a DNA microarray-based method with primer extension, named CAMpylobacter Express Resistance Array (CAMERA...
Tue, 12/22/2020 - 16:00
Poultry has been one of the major contributors of Campylobacter related human foodborne illness. Numerous interventions have been applied to limit Campylobacter colonization in poultry at the farm level, but other strategies are under investigation to achieve more efficient control. Probiotics are viable microbial cultures that can establish in the gastrointestinal tract (GIT) of the host animal...
Fri, 12/18/2020 - 10:02
Current approaches used for dose-response modeling of low-dose exposures of pathogens rely on assumptions and extrapolations. These models are important for quantitative microbial risk assessment of food. A mechanistic framework has been advocated as an alternative approach for evaluating dose-response relationships. The objectives of this study were to investigate the invasion behavior of...
Thu, 12/17/2020 - 10:02
Tetracyclines are widely used in veterinary medicine and food animal production. Campylobacter members are major foodborne pathogens, and their resistance to tetracycline has been widely reported in different countries. To date, Tet(O), a ribosomal protection protein, is the only confirmed Tet resistance determinant in Campylobacter spp. Here, we reported the detection and characterization of a...
Wed, 12/16/2020 - 10:03
Campylobacter jejuni is a zoonotic pathogen and is one of the leading causes of human gastroenteritis worldwide. C. jejuni IA3902 (representative of the sheep abortion clone) is genetically similar to C. jejuni W7 (representative of strain type NCTC 11168); however, there are significant differences in the ability of luxS mutants of these strains to colonize chickens. LuxS is essential for the...
Fri, 12/11/2020 - 16:00
Mononuclear molybdoenzymes are highly versatile catalysts that occur in organisms in all domains of life, where they mediate essential cellular functions such as energy generation and detoxification reactions. Molybdoenzymes are particularly abundant in bacteria, where over 50 distinct types of enzymes have been identified to date. In bacterial pathogens, all aspects of molybdoenzyme biology such...
Fri, 12/11/2020 - 06:05
Author(s): Zolzaya Byambajav, Erdenebat Bulgan, Yuji Hirai, Momoko Nakayama, Misaki Tanaka, Nitta Yurika, Akio Suzuki, Takashi Umemura, Bold Altankhuu, Alimaa Tsagaan, Batbaatar Vanaabaatar, Erdenebaatar Janchivdorj, Nyam-Osor Purevdorj, Narantuya Ayushjav, Takeshi Yamasaki, Motohiro Horiuchi There has been no report on the prevalence of Campylobacter spp. in farm animals in Mongolia. To uncover...
Wed, 12/09/2020 - 06:10
Author(s): Jimyeong Ha, Yeongeun Seo, Yujin Kim, Jeeyeon Lee, Heeyoung Lee, Sejeong Kim, Yukyung Choi, Hyemin Oh, Yewon Lee, Eunyoung Park, Joohyun Kang, Yohan Yoon The objective of this study was to develop a method with improved sensitivity for Campylobacter jejuni detection in foods. Nitrogen-doped carbon nanodots (N-CNDs) were synthesized and added to an enrichment medium (Bolton broth) at a...
Tue, 12/08/2020 - 16:00
Campylobacter jejuni express the high temperature requirement protein A (HtrA), a secreted serine protease, which is implicated in virulence properties of the pathogen. Previous studies have shown that C. jejuni HtrA can cleave the epithelial transmembrane proteins occludin and E-cadherin in the tight and adherens junctions, respectively. In the present report, we studied the interaction of HtrA...
Sun, 12/06/2020 - 06:12
Author(s): Shaimaa F. Mouftah, José F. Cobo-Díaz, Avelino Álvarez-Ordóñez, Ahmed Mousa, Jessica K. Calland, Ben Pascoe, Samuel K. Sheppard, Mohamed Elhadidy One of the emerging conundrums of Campylobacter food-borne illness is the bacterial ability to survive stressful environmental conditions. We evaluated the heterogeneity among 90 C. jejuni and 21 C. coli isolates from different sources in...
Thu, 12/03/2020 - 22:17
Antimicrobial resistance is a worldwide public health threat; hence, current trends tend to reduce antimicrobial use in food‐producing animals and to monitor resistance in primary production. This study aimed at evaluating the impact of antimicrobial use and production system on swine farms in the antimicrobial resistance of Campylobacter, Salmonella and Staphylococcus, the main zoonotic...
Thu, 11/26/2020 - 06:26
Author(s): Yizhi Tang, Yan Lai, Xingyuan Wang, Changwei Lei, Chao Li, Linghan Kong, Yulong Wang, Hongning Wang The optrA gene encodes an ABC-F protein which confers cross-resistance to oxazolidinones and phenicols, and its transmission has so far been associated with multiple transposable elements, including IS1216E, prophages, ICEs and Tn558. Here, we identified an optrA gene flanked by two...
Wed, 11/25/2020 - 10:02
ABSTRACT Antibiotic resistance (AR) remains a major threat to public and animal health globally. However, AR ramifications in developing countries are worsened by limited molecular diagnostics, expensive therapeutics, inadequate numbers of skilled clinicians and scientists, and unsanitary environments. The epidemiology of Gram-negative bacteria, their AR genes, and geographical distribution in...
Wed, 11/25/2020 - 10:02
Campylobacter is among the most common causes of gastroenteritis worldwide. Campylobacter jejuni and Campylobacter coli are the most common species causing human disease. DNA sequence-based methods for strain characterization have focused largely on C. jejuni, responsible for 80 to 90% of infections, meaning that C. coli epidemiology has lagged behind. Here, we have analyzed the genome of 450 C....
Wed, 11/25/2020 - 04:02
Numerous outbreak investigations and case-control studies of campylobacteriosis have provided evidence that handling Campylobacter -contaminated chicken products is a high risk factor for infection and illness. In this study, the cross-contamination and transfer rates of Campylobacter jejuni from chicken to ready-to-eat foods were determined in various food-handling scenarios. Skinless raw...
Tue, 11/24/2020 - 00:00
The ability of bacterial pathogens to form biofilms is an important virulence mechanism in relation to their pathogenesis and transmission. Biofilms play a crucial role in survival in unfavorable environmental conditions, acting as reservoirs of microbial contamination and antibiotic resistance. For intestinal pathogen Campylobacter jejuni, biofilms are considered to be a contributing factor in...
Mon, 11/23/2020 - 22:17
Campylobacter is a leading cause of foodborne illness in humans worldwide. Sources of infection are often difficult to identify, and are, generally, poorly understood. Recent work suggests that wildlife may represent a source of Campylobacter for human infections. Using a repeated cross‐sectional study design, raccoons were trapped on five swine farms and five conservation areas in southern...
Sat, 11/21/2020 - 06:24
Author(s): Maria João Cardoso, Vânia Ferreira, Mónica Truninger, Rui Maia, Paula Teixeira Contaminated poultry is the major vehicle for consumer's exposure to Campylobacter. This study aimed to perceive potential cross-contamination events during preparation of raw poultry that can contribute to the spread of Campylobacter spp. in domestic kitchen environments and to understand consumers'...
Sat, 11/21/2020 - 00:00
Antibiotic resistance is an important issue affecting humans and livestock. Antimicrobial peptides are promising alternatives to antibiotics. In this study, the antimicrobial peptide Css54, isolated from the venom of C. suffuses, was found to exhibit antimicrobial activity against bacteria such as Listeria monocytogenes, Streptococcus suis, Campylobacter jejuni, and Salmonella typhimurium that...
Fri, 11/20/2020 - 06:10
Author(s): K. Hertogs, M. Heyndrickx, P. Gelaude, L. De Zutter, J. Dewulf J, G. Rasschaert Poultry is seen as the main reservoir for Campylobacter. Control of this zoonotic pathogen in primary production could potentially reduce the colonization in broiler flocks and consequently reduce the number of human infections. In the present study, 20 broiler flocks from ten farms, were sampled...
Thu, 11/19/2020 - 16:00
Campylobacter is the leading cause of the human bacterial foodborne infections in the developed countries. The perception cues from biotic or abiotic environments by the bacteria are often related to bacterial surface and membrane proteins that mediate the cellular response for the adaptation of Campylobacter jejuni to the environment. These proteins function rarely as a unique entity, they are...
Mon, 11/16/2020 - 16:00
The microaerophilic pathogen Campylobacter jejuni is a leading bacterial cause of human gastroenteritis in developed countries. Even though it has a reputation as a fastidious organism, C. jejuni is widespread and can be easily isolated from various animals, food, and environmental sources. It is suggested that an ability to form biofilms is probably necessary for the survival of C. jejuni under...
Fri, 11/13/2020 - 04:02
Campylobacteriosis is the most frequently reported foodborne illness in Europe and many other parts of the world. Campylobacter can colonize the intestines of broilers, most often in large amounts. Broilers are usually slaughtered in a high-speed automated system that can rupture the intestines during evisceration, resulting in contamination of carcasses with intestinal bacteria such as...
Fri, 11/06/2020 - 16:00
Evolution experiments in the laboratory have focused heavily on model organisms, often to the exclusion of clinically relevant pathogens. The foodborne bacterial pathogen Campylobacter jejuni belongs to a genus whose genomes are small compared to those of its closest genomic relative, the free-living genus Sulfurospirillum, suggesting genome reduction during the course of evolution to host...
Fri, 11/06/2020 - 06:13
Author(s): Ross C. Beier, J. Allen Byrd, Kathleen Andrews, Denise Caldwell, Tawni L. Crippen, Robin C. Anderson, David J. Nisbet Foodborne illness is an on-going problem worldwide and is caused by bacteria that invade the food chain from the farm, slaughter house, restaurant or grocery, or in the home and can be controlled by strategies using biocides (antiseptics and disinfectants)....
Thu, 11/05/2020 - 16:00
As a leading cause of bacterial-derived gastroenteritis worldwide, Campylobacter jejuni has a significant impact on human health in both the developed and developing worlds. Despite its prevalence as a human pathogen, the source of these infections remains poorly understood due to the mutation frequency of the organism and past limitations of whole genome analysis. Recent advances in both whole...
Thu, 11/05/2020 - 12:01
Background Campylobacter species are among the most common causes of enteric bacterial infections worldwide. Men who have sex with men (MSM) are at increased risk for sexually transmitted enteric infections, including globally distributed strains of multidrug-resistant Shigella species. Methods This was a retrospective study of MSM-associated Campylobacter in Seattle, Washington and Montréal,...
Sun, 11/01/2020 - 06:13
Author(s): Takako Taniguchi, Mayuko Ohki, Ayaka Urata, Shoutaro Ohshiro, Elpita Tarigan, Savek Kiatsomphob, Torrung Vetchapitak, Hiroyuki Sato, Naoaki Misawa Campylobacter jejuni is the leading cause of bacterial food poisoning worldwide. Chickens are considered to be one of the major reservoirs of Campylobacter infection in humans due to colonization of their intestinal tract. When the chickens...
Sat, 10/31/2020 - 04:02
Poultry processing establishments utilize antimicrobial processing aids on broiler parts to minimize Campylobacter contamination. A stabilized hydrogen peroxide (SHP) product was assessed for use as an antimicrobial processing aid. In a series of experiments, wing segments with skin were inoculated with 103 - 107 cells of Campylobacter coli followed by treatment with SHP at 15,000 or 30,000 mg/L...
Fri, 10/30/2020 - 22:17
Aims Cattle are the second most important cause of human campylobacteriosis, after poultry, but there are knowledge gaps regarding Campylobacter in cattle. This study examined the occurrence of Campylobacter, the species present, sequence types, and antibiotic resistance in Swedish cattle. Methods and Results Feces samples collected from 154 calves on seven Swedish farms, and 69 follow‐up samples...
Thu, 10/29/2020 - 16:00
Campylobacter infections are the leading cause of bacterial gastroenteritis. In Europe, over 246,000 cases are confirmed annually. Infections are often transmitted via contaminated food, such as poultry products, but water may be the source of infection as well. The aim of this study was to characterise a selection of Campylobacter jejuni human isolates, together with a water isolate, from a...
Thu, 10/29/2020 - 10:03
Zinc oxide nanoparticles (ZnO NPs) are regarded as a safe and stable antimicrobial that can inactivate bacteria by several potential working mechanisms. We aimed to incorporate ZnO NPs into packaging material to control Campylobacter in raw chicken meat. ZnO NPs were first incorporated into three-dimensional (3D) paper tubes to identify the lethal concentration against Campylobacter jejuni, which...
Wed, 10/28/2020 - 16:00
Despite continued efforts to improve biosecurity protocols, Campylobacter continues to be detected in the majority of commercial chicken flocks across Europe. Using an extensive data set of Campylobacter prevalence within a chicken breeder flock for over a year, multiple Bayesian models are presented to explore the dynamics of the spread of Campylobacter in response to seasonal variation, species...
Tue, 10/27/2020 - 08:03
by Deepti Pranay Samarth, Young Min Kwon Many epidemiological studies provide us with the evidence of horizontal gene transfer (HGT) contributing to the bacterial genomic diversity that benefits the bacterial populations with increased ability to adapt to the dynamic environments. Campylobacter jejuni, a major cause of acute enteritis in the U.S., often linked with severe post-infection...
Thu, 10/22/2020 - 02:03
The microbiological contamination of food causes concern for public health due to the pathogenic action of microorganisms and the increasing of antimicrobial resistance observed in bacterial strains. This work considers the use of fluorescence spectroscopy to study the effects of the presence of microorganisms in the chicken’s meat and therefore, how the detection of this presence can help on...
Mon, 10/19/2020 - 16:00
Campylobacter species are zoonotic pathogens and the leading cause of bacterial enteritis worldwide. With the increase of antimicrobial resistance to fluoroquinolones and macrolides, they have been identified by the World Health Organization (WHO) as high-priority antimicrobial-resistant pathogens. There is currently little known about the prevalence and antimicrobial resistance characteristics...
Mon, 10/19/2020 - 16:00
The formation of persister cells is one mechanism by which bacteria can survive exposure to environmental stresses. We show that Campylobacter jejuni 11168H forms persister cells at a frequency of 10−3 after exposure to 100 × MIC of penicillin G for 24 h. Staining the cell population with a redox sensitive fluorescent dye revealed that penicillin G treatment resulted in the appearance of a...
Sat, 10/17/2020 - 08:02
by Heba Barnawi, Nader Masri, Natasha Hussain, Bushra Al-Lawati, Evita Mayasari, Aleksandra Gulbicka, Adrian J. Jervis, Min-Hsuan Huang, Jennifer S. Cavet, Dennis Linton RNA thermometers (RNATs) trigger bacterial virulence factor expression in response to the temperature shift on entering a warm-blooded host. At lower temperatures these secondary structures sequester ribosome-binding sites (RBSs...

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