Campylobacter

Mon, 10/19/2020 - 16:00
Campylobacter species are zoonotic pathogens and the leading cause of bacterial enteritis worldwide. With the increase of antimicrobial resistance to fluoroquinolones and macrolides, they have been identified by the World Health Organization (WHO) as high-priority antimicrobial-resistant pathogens. There is currently little known about the prevalence and antimicrobial resistance characteristics...
Mon, 10/19/2020 - 16:00
The formation of persister cells is one mechanism by which bacteria can survive exposure to environmental stresses. We show that Campylobacter jejuni 11168H forms persister cells at a frequency of 10−3 after exposure to 100 × MIC of penicillin G for 24 h. Staining the cell population with a redox sensitive fluorescent dye revealed that penicillin G treatment resulted in the appearance of a...
Sat, 10/17/2020 - 08:02
by Heba Barnawi, Nader Masri, Natasha Hussain, Bushra Al-Lawati, Evita Mayasari, Aleksandra Gulbicka, Adrian J. Jervis, Min-Hsuan Huang, Jennifer S. Cavet, Dennis Linton RNA thermometers (RNATs) trigger bacterial virulence factor expression in response to the temperature shift on entering a warm-blooded host. At lower temperatures these secondary structures sequester ribosome-binding sites (RBSs...
Fri, 10/16/2020 - 06:16
Author(s): Benjamin Duqué, Justine Canon, Nabila Haddad, Sandrine Guillou, Jeanne-Marie Membré Predictive modelling is used in microbiological risk assessment to quantify the growth and inactivation of microorganisms through the use of mathematical models. Campylobacter jejuni is one of the main foodborne pathogens and broiler meat is considered as the most important source of human...
Thu, 10/15/2020 - 16:00
The present study investigated the genomic constitution and antimicrobial resistance (AMR) of 238 Campylobacter from pigs and wild boars in Italy between 2012 and 2019. Campylobacter strains were genotyped using multilocus sequence typing (MLST) and whole genome MLST (wgMLST), screened for antimicrobial resistance genes, and tested for phenotypic susceptibility to six different antibiotics. C....
Thu, 10/15/2020 - 00:01
The zoonotic human pathogen Campylobacter jejuni is known for its ability to induce DNA-damage and cell death pathology in humans. The molecular mechanism behind this phenomenon involves nuclear translocation by Cas9, a nuclease in C. jejuni (CjeCas9) that is the molecular marker of the Type II CRISPR-Cas system. However, it is unknown via which cellular pathways CjeCas9 drives human intestinal...
Thu, 10/08/2020 - 10:04
In this report, we present the whole-genome sequence of a Campylobacter jejuni strain isolated recursively for the last 3 years from an Italian poultry farm.
Thu, 10/08/2020 - 10:04
Here, we report the draft genome sequences of robust (A74/C_24-3) and poor (A74/O_2-2) chicken-colonizing Campylobacter jejuni isolates. Whole-genome sequence analyses of these isolates will be helpful in facilitating further studies to identify genetic factors used in chicken colonization.
Thu, 10/08/2020 - 06:23
Author(s): S. Vaddu, J. Kataria, E.N. Rama, A.E. Moller, A. Gouru, M. Singh, H. Thippareddi Peroxy acetic acid (PAA) is widely used as an antimicrobial in poultry processing, specifically in the chiller. While the natural pH of PAA at the concentrations used is between 4.5 and 6.0, poultry processors adjust the pH to ≥8.0 to maintain product yield. The objective of this study was to evaluate (i)...
Fri, 10/02/2020 - 10:03
In September 2018, Hurricane Florence caused extreme flooding in eastern North Carolina, USA, a region highly dense in concentrated animal production, especially swine and poultry. In this study, floodwater samples (n = 96) were collected as promptly post-hurricane as possible and for up to approximately 30 days and selectively enriched for Campylobacter using Bolton broth enrichment and...
Fri, 10/02/2020 - 10:03
The present multicenter study aimed at assessing the performance of air sampling as a novel method for monitoring Campylobacter in biosecure poultry farms. We compared, using a harmonized procedure, the bacteriological isolation protocol (ISO 10272-1:2017) and a real-time PCR method used on air filter samples. Air samples and boot swabs were collected from 62 biosecure flocks from five European...
Fri, 10/02/2020 - 10:02
Rapid and accurate identification of Arcobacter is of great importance because it is considered an emerging food- and waterborne pathogen and potential zoonotic agent. Raman spectroscopy can differentiate bacteria based on Raman scattering spectral patterns of whole cells in a fast, reagentless, and easy-to-use manner. We aimed to detect and discriminate Arcobacter bacteria at the species level...
Sun, 09/27/2020 - 06:15
Author(s): L.K. Froebel, L.E. Froebel, T. Duong The development of interventions to reduce human foodborne pathogens in the gastrointestinal (GI) tract of chickens will be important for improving the microbial food safety of poultry. Saccharomyces-derived prebiotic Refined Functional Carbohydrates (RFC), composed primarily of β-glucans, mannanoligosaccharides (MOS), and D-mannose, have been...
Fri, 09/25/2020 - 22:16
SUMMARY Microbiological contamination of chicken meat depends on the conditions under which the animals are reared, slaughtered and processed. The aim of this study was to determine the influence of farm origin and processing stages at slaughterhouse on the microbial safety and quality of chicken. Samples of chicken carcasses from three different farms were taken from a slaughterhouse. Mesophiles...
Thu, 09/24/2020 - 16:00
Vibrio parahaemolyticus non-toxigenic strains are responsible for about 10% of acute gastroenteritis associated with this species, suggesting they harbor unique virulence factors. Zonula occludens toxin (Zot), firstly described in Vibrio cholerae, is a secreted toxin that increases intestinal permeability. Recently, we identified Zot-encoding genes in the genomes of highly cytotoxic Chilean V....
Wed, 09/23/2020 - 22:17
Campylobacter spp. is the leading cause of bacterial gastroenteritis worldwide and poultry are the primary reservoir. The aim of this study was to investigate the survival and/or growth of C. jejuni NCTC 11168 in broiler digestate prepared from commercial starter, grower and finisher feed formulations. Bolton broth and digestates were prepared, inoculated with C. jejuni NCTC 11168 (approximately...
Wed, 09/23/2020 - 14:10
Campylobacter jejuni is a predominant zoonotic pathogen causing gastroenteritis and other diseases in humans. An important bacterial virulence factor is the secreted serine protease HtrA (HtrA Cj ), which targets tight and adherens junctional proteins in the gut epithelium. Here we have investigated the function and structure of HtrA Cj using biochemical assays and cryo-electron microscopy. Mass...
Wed, 09/23/2020 - 10:03
The bacteriological diagnosis of intestinal bacterial infections has historically been based on culture on agar plates. However, culture may lack sensitivity, and some enteropathogens, such as pathovars of Escherichia coli, may escape routine diagnosis. Our goal was to evaluate the analytical performance of the Novodiag Bacterial GE+ kit for the detection of enteropathogenic bacteria in acute...
Wed, 09/23/2020 - 10:03
Campylobacter jejuni is a leading cause of enteric bacterial illness in the United States. Traditional molecular subtyping methods, such as pulsed-field gel electrophoresis (PFGE) and 7-gene multilocus sequence typing (MLST), provided limited resolution to adequately identify C. jejuni outbreaks and separate out sporadic isolates during outbreak investigations. Whole-genome sequencing (WGS) has...
Fri, 09/18/2020 - 08:02
by Elisa Peh, Sophie Kittler, Felix Reich, Corinna Kehrenberg Contaminated poultry meat is considered to be the main source of human infection with Campylobacter spp., a pathogen that asymptomatically colonizes broiler chickens during fattening and contaminates carcasses during slaughter. To prevent or reduce the colonization of broiler flocks with Campylobacter spp., applying different organic...
Wed, 09/16/2020 - 22:19
Significance and Impact of the Study: Campylobacter fetus subsp. venerealis (Cfv) is frequently associated with lowered fertility and abortion in ruminants. These infections are responsible for significant reproductive wastage in infected beef and dairy herds and may reduce gross profit margins up to 66%. In India, decreased fertility rates in productive animals are traditionally attributed to...
Mon, 09/14/2020 - 06:15
Author(s): L.C. Eastwood, T.M. Taylor, J.W. Savell, K.B. Gehring, A.N. Arnold Effect of various antimicrobial interventions on pork carcass cuts – skin-on and skinless, non-chilled and chilled – was studied. Carcass pieces were inoculated with Salmonella enterica, Shiga toxin-producing Escherichia coli (STEC), Escherichia coli pathogen surrogates or Campylobacter spp. Inoculated pieces were...
Fri, 09/11/2020 - 16:00
We compared the consistency, accuracy and reproducibility of next-generation short read sequencing between ten laboratories involved in food safety (research institutes, state laboratories, universities and companies) from Germany and Austria. Participants were asked to sequence six DNA samples of three bacterial species (Campylobacter jejuni, Listeria monocytogenes and Salmonella enterica) in...
Sat, 09/05/2020 - 22:25
The research aimed to generate an early warning system highlighting in real‐time bacterial contamination of meat matrices and providing information which could support companies in accepting or rejecting batches. Current microorganisms’ detection methods rely on techniques (plate counting), which provide retrospective values for microbial contamination. The purpose of this research was to...
Sat, 09/05/2020 - 22:18
Aim To analyse the prevalence, genetic diversity and antimicrobial susceptibility of Campylobacter spp. in northern Spain. Methods and results Campylobacter was isolated from 139 samples of broiler meat and faecal dropping of broiler and swine with a prevalence of 35.4%, 62% and 42.8%, respectively. Campylobacter jejuni (n=55) and Campylobacter coli (n=31) were identified by multiplex‐PCR in meat...
Sat, 09/05/2020 - 14:08
The symptoms of infectious diarrheal disease are mediated by a combination of a pathogen’s virulence factors and the host immune system. Campylobacter jejuni is the leading bacterial cause of diarrhea worldwide due to its near-ubiquitous zoonotic association with poultry. One of the outstanding questions is to what extent the bacteria are responsible for the diarrheal symptoms via intestinal cell...
Fri, 08/28/2020 - 06:11
Author(s): Wageha A. Awad, Daniel Ruhnau, Claudia Hess, Michael Hess In recent years several studies emphasize on the deleterious effects of Campylobacter jejuni on the chicken intestine. In this context it was shown that Campylobacter jejuni, contrary to the general belief, has a negative influence on the gut barrier in chickens. More precisely, we demonstrated that C. jejuni affects gut...
Tue, 08/25/2020 - 10:05
ABSTRACT The crust is the outermost spore layer of most Bacillus strains devoid of an exosporium. This outermost layer, composed of both proteins and carbohydrates, plays a major role in the adhesion and spreading of spores into the environment. Recent studies have identified several crust proteins and have provided insights about their organization at the spore surface. However, although...
Mon, 08/24/2020 - 18:12
Aims: This study aimed to describe the demographic and geographic patterns of campylobacteriosis in Denmark, Finland, Norway and Sweden during 2000–2015. Methods: All Campylobacter infections notified to national authorities in the four countries during the study period were included. Background data for each notification consisted of patient age, sex, geographical location, presumed origin of...
Sat, 08/15/2020 - 12:00
Background Campylobacter infection is associated with impaired growth of children, even in the absence of symptoms. To examine the underlying mechanisms, we evaluated associations between Campylobacter infection, linear growth, and fecal microbial community features in a prospective birth cohort of 271 children with a high burden of diarrhea and stunting in the Amazonian lowlands of Peru. Methods...
Thu, 08/13/2020 - 16:00
Rapid developments in the field of whole genome sequencing (WGS) make in silico antimicrobial resistance (AMR) a target within reach. Campylobacter jejuni is a leading cause of foodborne infections in Israel with increasing rates of resistance. We applied WGS analysis to study the prevalence and genetic basis of AMR in 263 C. jejuni human and veterinary representative isolates retrieved from a...
Sat, 08/08/2020 - 04:02
The prevalence was determined of Campylobacter jejuni and C. coli on three age classes (lamb, hogget and mutton) of ovine carcass trim post-dressing and pre-chill. Sampling of hogget carcasses was undertaken six months before sampling of lamb and mutton carcasses. A total of 120 trim samples was collected from 11 processing plants across New Zealand (NZ). All samples were enriched and screened...
Tue, 08/04/2020 - 16:00
Campylobacteriosis is one of the most common causes of bacterial gastroenteritis. However, the clinical course of the illness varies in symptoms and severity. The aim of this study was to characterize Campylobacter jejuni (34 isolates) and C. coli (9 isolates) from persons with diarrheal and non-diarrheal stools at the time of examination and fecal sampling, in Poland by using whole-genome...
Tue, 08/04/2020 - 14:07
The aim of this study was to evaluate the influence of Lactobacillus reuteri B1/1 on the local intestinal immune response in the cecum of chickens experimentally infected by C. jejuni CCM6189. Day-old chicken were divided into four groups: control (C), L. reuteri B1/1 (LR), C. jejuni CCM6189 (CJ), and combined L. reuteri B1/1 + C. jejuni CCM6189 (LRCJ). Cecal samples were collected during...
Sun, 08/02/2020 - 06:13
LWT
Author(s): Wentao Jiang, KaWang Li, Yu-Chun Chiu, Carly Waldman, Cangliang Shen This study aimed to evaluate the thermal inactivation of Campylobacter jejuni in moisture enhanced reconstructed non-intact chicken patties with various pump rates and double pan-broiled at different temperatures. Fresh 1.5-kg coarse-ground chicken breast, inoculated with C. jejuni (3-strain mixture), were moisture...
Sat, 08/01/2020 - 06:19
Author(s): Jasmine Kataria, Sasikala Vaddu, Estefania Novoa Rama, Gaganpreet Sidhu, Harshavardhan Thippareddi, Manpreet Singh Peracetic acid (PAA) is commonly used as an antimicrobial during poultry processing to reduce the pathogen load on poultry and poultry products. However, limited research is available on the effects of pH on the efficacy of PAA against Salmonella and Campylobacter....
Fri, 07/31/2020 - 08:02
by Rhiannon L. Wallace, Dieter M. Bulach, Amy V. Jennison, Mary Valcanis, Angus McLure, James J. Smith, Trudy Graham, Themy Saputra, Simon Firestone, Sally Symes, Natasha Waters, Anastasia Stylianopoulos, Martyn D. Kirk, Kathryn Glass Australian rates of campylobacteriosis are among the highest in developed countries, yet only limited work has been done to characterize Campylobacter spp. in...
Thu, 07/30/2020 - 22:14
ABSTRACT Aims This study evaluated the performance of a commercial molecular detection method (mericon Campylobacter triple kit real‐time/quantitative (q)PCR) and a selective plating medium (R&F Campylobacter jejuni/Campylobacter coli Chromogenic Plating Medium (CCPM)) against a culture‐based reference method (ISO 10272‐1:2017 detection procedure B) for the detection of Campylobacter from raw...
Thu, 07/30/2020 - 04:02
Herein, a high-affinity single-stranded DNA aptamer (59 nt) against Campylobacter jejuni, defined as CJA1, was obtained using the whole-bacterium-based systemic evolution of ligands by exponential enrichment procedure. CJA1 was analyzed with a stable secondary structure and low dissociation constant (Kd) value of 1.37 ± 0.28 nM. The potential use of CJA1 was exemplified by the construction of a...
Thu, 07/23/2020 - 10:02
Campylobacter jejuni is the leading cause of bacterial foodborne disease worldwide. Here, we report the complete annotated genomes and plasmid sequences of 17 Campylobacter jejuni strains isolated from patients with gastroenteritis in Santiago, Chile.
Wed, 07/22/2020 - 08:02
Vertebrates, from zebra fish to humans, have an innate immune recognition of many bacterial flagellins. This involves a conserved eight-amino acid epitope in flagellin recognized by the Toll-like receptor 5 (TLR5). Several important human pathogens, such as Helicobacter pylori and Campylobacter jejuni, have escaped TLR5 activation by mutations in this...
Wed, 07/22/2020 - 00:00
Campylobacter species are among the major bacteria implicated in human gastrointestinal infections and are majorly found in faeces of domestic animals, sewage discharges and agricultural runoff. These pathogens have been implicated in diseases outbreaks through consumption of contaminated milk and water in some parts of the globe and reports on this is very scanty in the Eastern Cape Province....
Sat, 07/18/2020 - 08:02
The objective of this study was to compare the prevalence and antimicrobial resistance (AMR) rates of Campylobacter spp. isolated from conventional and organic turkey meat sold at retail in Germany. Samples of conventional (N = 527) and organic (N = 245) fresh turkey meat without skin were collected at retail markets throughout Germany and tested for Campylobacter spp.. Campylobacter isolates...
Sat, 07/11/2020 - 04:02
In Japan, strategies for ensuring food safety have been developed without reliable scientific evidence on the relation between foodborne infections and foods. The aim of this research was to provide information on the proportions of foodborne diseases caused by seven major causative pathogens ( Campylobacte r spp., Salmonella spp., EHEC, V. parahaemolyticus , Clostridium perfringens ,...
Fri, 07/10/2020 - 08:02
by Mihkel Mäesaar, Triin Tedersoo, Kadrin Meremäe, Mati Roasto The reservoir and source of human campylobacteriosis is primarily considered to be poultry, but also other such as ruminants, pets and environmental sources are related with infection burden. Multilocus sequence typing is often used for Campylobacter epidemiological studies to determine potential sources of human infections. The...
Fri, 07/10/2020 - 04:02
As one of the main causes of bacterial diarrhea and a major risk factor for triggering Guillain–Barré autoimmune syndrome, campylobacteriosis, that is, Campylobacter spp. infections, represents a major health issue worldwide. There is thus a pressing need for developing an effective and broad-coverage campylobacteriosis vaccine. Campylobacter jejuni, an encapsulated, multidrug resistant Gram-...
Thu, 07/09/2020 - 08:02
Campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide with a very low outbreak reported in China. In May 2019, we investigated an outbreak of Campylobacter jejuni infections among students in a junior high school in Eastern China. Cases were interviewed to identify a common source of contamination. As cases were identified in the same school...
Thu, 07/09/2020 - 06:13
Author(s): C. Marin, S. Sevilla-Navarro, R. Lonjedo, P. Catalá-Gregori, M.A. Ferrús, S. Vega, A. Jiménez-Belenguer Thermophilic Campylobacter species are recognised as a major cause of acute bacterial diarrhoea in humans, being broiler meat the most common source of human infection. Antibiotic therapy is usually necessary for severe or prolonged infections, especially in immunocompromised...
Thu, 07/09/2020 - 00:00
Tea Tree oil (TTO) is well known for its numerous good properties but might be also irritating or toxic when used topically or ingested, thus limiting the number of possible applications in Humans. The aim of the study was to characterize the antimicrobial spectrum as well as the toxicity of Titroleane™, a new anti-infective agent obtained from TTO but cleared of its toxic monoterpenes...
Wed, 07/08/2020 - 04:02
Results from monitoring of the microbiological quality of 2,721 samples of ready-to-eat cooked chicken collected between 2013 to 2017 in England were reviewed: 70% of samples were from retail, catering or manufacture and 30% were imported and collected at English ports. Samples were tested for a range of bacterial pathogens and indicator organisms. Six samples (<1%) had unsatisfactory levels...

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