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Campylobacter

Fri, 05/24/2019 - 11:00
Although enteric multianalyte syndromic panels are increasingly employed, direct comparisons with traditional methods and the inclusion of host phenotype correlations are limited. Luminex xTAG gastrointestinal pathogen panel (GPP) and culture results are highly concordant. However, phenotypic and microbiological confirmatory testing raises concerns regarding the accuracy of the GPP, especially...
Fri, 05/24/2019 - 01:11
Publication date: August 2019Source: LWT, Volume 111Author(s): Mohammad Amin Nouri Ala, Yasser ShahbaziAbstractThe aims of the present study were to evaluate the effects of carboxymethyl cellulose (CMC) coatings containing Ziziphora clinopodioides (ZEO; 0, 0.25 and 0.5%) and Mentha spicata (MEO; 0 and 0.5%) essential oils on pathogenic bacteria (i.e. Listeria monocytogenes, Staphylococcus aureus...
Wed, 05/22/2019 - 15:00
Abstract Susceptibility profiles were determined for 111 Campylobacter coli strains obtained in 1998 to 1999 and 2015 from market age pigs and pork chops against 22 disinfectants and 9 antimicrobials. Resistance to tetracycline (TET) was observed in 44.4% of 1998 to 1999 strains, and the antibiotic resistance profile was TET. But strains obtained in 2015 from swine and retail pork chops had 75%...
Fri, 05/17/2019 - 15:30
The goal of this study was to evaluate factors causing low recovery of Campylobacter during regulatory sampling following acidified sodium chlorite (ASC) treatment of broiler carcasses and subsequent carry‐over into neutralizing buffered peptone water (nBPW) rinses. Solution alkalinity was shown to positively correlate with the presence of un‐reduced chlorite anion in the recovery broth,...
Fri, 05/17/2019 - 15:30
The goal of this study was to evaluate factors causing low recovery of Campylobacter during regulatory sampling following acidified sodium chlorite (ASC) treatment of broiler carcasses and subsequent carry‐over into neutralizing buffered peptone water (nBPW) rinses. Solution alkalinity was shown to positively correlate with the presence of un‐reduced chlorite anion in the recovery broth,...
Fri, 05/17/2019 - 01:12
Available online 15 May 2019 Author(s): Simone Buess, Katrin Zurfluh, Roger Stephan, Claudia GuldimannAbstractWe performed a quantitative microbiological slaughter process analysis in a large-scale Swiss poultry abattoir. At each of six selected process steps (after plucking, cloaca excision, evisceration, washing, water spray chiller and dry air chiller), 103 carcasses from 50 flocks were...
Thu, 05/16/2019 - 20:00
Campylobacter jejuni infections constitute serious threats to human health with increasing prevalences worldwide. Our knowledge regarding the molecular mechanisms underlying host–pathogen interactions is still li...
Thu, 05/16/2019 - 11:01
Campylobacter is a major foodborne pathogen in humans and a significant cause of abortion in sheep. Although ruminants are increasingly recognized as important reservoirs for Campylobacter species, limited information is available about the molecular epidemiology and antimicrobial resistance (AMR) profiles of sheep Campylobacter. Here, we describe a two-trial study that examined Campylobacter...
Tue, 05/14/2019 - 09:32
Eleutherine americana, a well‐known Asian medicinal herb, has been claimed for many biological activities. In this study, potential of E. americana ethanolic extract against adhesion and invasion of Caco‐2 cells by commonly contaminated Campylobacter spp. was evaluated. MIC50 values of E. americana extract against 39 enteritis patient and 26 chicken isolates were 125 µg/ml. Two models were set up...
Mon, 05/13/2019 - 20:00
The aim of this study is to investigate the antimicrobial synergistic effect against Campylobacter jejuni, a leading foodborne pathogen that causes human gastroenteritis, by cinnamon oil, encapsulated curcumin, and zinc oxide nanoparticles (ZnO NPs). We compared three approaches to study the antimicrobial interactions, including the time-killing method, the fractional inhibitory concentration...
Thu, 05/09/2019 - 10:29
Campylobacter jejuni helical shape is key to its pathogenesis. Under stress, C. jejuni alters its shape from helical to coccoid. This study characterized C. jejuni bacterial cell wall peptidoglycan changes occurring during coccoid formation and identified a role for the peptidoglycan hydrolases Pgp1 and previously uncharacterized AmiA in the process. In addition, we determined that coccoid C....
Sun, 05/05/2019 - 20:00
Campylobacter is the most common cause of traveler’s diarrhea (TD) and human bacterial gastroenteritis. A heteroresistant Campylobacter jejuni (C. jejuni) isolate, identified by microbiological methods and charac...
Thu, 05/02/2019 - 11:02
Enterobactin (Ent)-mediated high-affinity iron acquisition is critical for Gram-negative bacteria to survive in the host. Given the bacteriostatic effect of lipocalin resulting from its potent Ent-binding ability, immune intervention directly targeting Ent is promising for iron-dependent pathogen control. Recently, an Ent conjugate vaccine was reported, but it still has several significant...
Wed, 05/01/2019 - 01:10
Available online 29 April 2019 Author(s): Xinfeng Han, Xingai Guan, Hang Zeng, Jiakang Li, Xuelin Huang, Yiping Wen, Qin Zhao, Xiaobo Huang, Qigui Yan, Yong Huang, Sanjie Cao, Rui Wu, Xiaoping Ma, Likou ZouAbstractA total of 220 Campylobacter isolates was recovered from 656 samples along a commercial duck slaughter line after a panel of confirmation tests including selective culture methods,...
Tue, 04/30/2019 - 20:00
Random Mutagenesis Applied to Reveal Factors Involved in Oxidative Tolerance and Biofilm Formation in Foodborne Cronobacter malonaticus Maofeng Zhang1,2†, Xiyan Zhang1†, Liaowang Tong1†, Dexin Ou1, Yaping Wang1, Jumei Zhang2, Qingping Wu2* and Yingwang Ye1,2* 1School of Food Science and Engineering, Hefei University of Technology, Hefei, China 2State Key Laboratory of...
Tue, 04/30/2019 - 01:14
Available online 28 April 2019 Author(s): G. Douglas Inglis, Valerie F. Boras, Andrew L. Webb, Vivien V. Suttorp, Pamela Hodgkinson, Eduardo N. TaboadaAbstractInfections by pathogenic Campylobacter species were determined in diarrheic (n = 2,217) and non-diarrheic control (n = 104) people in Southwestern Alberta (SWA) over a 1-year period using specialized and conventional isolation, and direct...
Thu, 04/25/2019 - 20:00
Role of Starter Cultures on the Safety of Fermented Meat Products Marta Laranjo1*, Maria Eduarda Potes1,2 and Miguel Elias1,3 1ICAAM-Instituto de Ciências Agrárias e Ambientais Mediterrânicas, Universidade de Évora, Pólo da Mitra, Évora, Portugal 2Departamento de Medicina Veterinária, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo...
Wed, 04/24/2019 - 20:00
Originating from poultry, particularly chickens, Campylobacter jejuni is the leading foodborne pathogen worldwide and a major cause of campylobacteriosis. Isolating C. jejuni is difficult due to its specific grow...
Tue, 04/16/2019 - 20:00
Structural and Proteomic Changes in Viable but Non-culturable Vibrio cholerae Susanne Brenzinger1*, Lizah T. van der Aart1, Gilles P. van Wezel1, Jean-Marie Lacroix2, Timo Glatter3 and Ariane Briegel1* 1Department of Microbial Biotechnology & Health, Institute of Biology Leiden, Leiden University, Leiden, Netherlands 2Unité de Glycobiologie Structurale et Fonctionnelle, UMR CNRS 8576...
Mon, 04/15/2019 - 15:00
Abstract The role of free‐ranging wildlife in the epidemiology of enteropathogens causing clinical illness in humans and domestic animals is unclear. Salmonella enterica and anti‐microbial resistant bacteria have been detected in the faeces of raccoons (Procyon lotor), but little is known about the carriage of these bacteria in other sympatric meso‐mammals. Our objectives were to: (a) report the...
Sun, 04/14/2019 - 01:12
Available online 13 April 2019 Author(s): Cangliang Shen, Lacey Lemonakis, Xiaoli Etienne, KaWang Li, Wentao Jiang, Jeremy M. AdlerAbstractThis study compares the efficacy of commercial antimicrobials to inactivate unstressed and stress-adapted Campylobacter jejuni on broiler wings using immersion and electrostatic spray and evaluates the economic feasibility of the two methods. Overnight...
Thu, 04/11/2019 - 20:00
Campylobacter jejuni is a major food-borne pathogen and a worldwide health threat. Utilizing different virulence factors, C. jejuni invades the host’s intestinal epithelial cell layer. One important factor in thi...
Wed, 04/10/2019 - 20:00
Campylobacter jejuni Demonstrates Conserved Proteomic and Transcriptomic Responses When Co-cultured With Human INT 407 and Caco-2 Epithelial Cells Nicholas M. Negretti1, Geremy Clair2, Prabhat K. Talukdar1, Christopher R. Gourley1, Steven Huynh3, Joshua N. Adkins2, Craig T. Parker3, Colby M. Corneau1 and Michael E. Konkel1* 1School of Molecular Biosciences, College of Veterinary Medicine,...
Tue, 04/09/2019 - 20:00
Identification of a nth-Like Gene Encoding an Endonuclease III in Campylobacter jejuni Lei Dai1, Jing Xia1,2, Orhan Sahin3 and Qijing Zhang1* 1Departments of Veterinary Microbiology and Preventive Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United States 2National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, College of...
Sat, 03/30/2019 - 01:13
Anna-Maria Schmidt, Ulrike Escher, Soraya Mousavi, Manja Boehm, Steffen Backert, Stefan Bereswill, Markus M. Heimesaat
Thu, 03/28/2019 - 11:00
Enteropathogenic Escherichia coli (EPEC) is a major cause of diarrhea in children from developing countries and presents high genetic variability. We aimed to characterize the EPEC virulence-related gene (VRG) distribution and copathogens associated with diarrhea and nutrition-related outcomes in children from the low-income Brazilian semiarid region. A cross-sectional case-control study of...
Thu, 03/28/2019 - 01:13
Publication date: 15 August 2019 , Volume 289Author(s): Deyun He, Zhengzong Wu, Bo Cui, Enbo Xu, Zhengyu JinAbstractCampylobacter jejuni (C. jejuni) is considered one of the most common cause of human gastroenteritis. Aiming to detect C. jejuni in food products rapidly and sensitively, a dual mode lateral flow assay, based on the peroxidase mimicking and surface enhanced Raman scattering (SERS)...
Mon, 03/25/2019 - 17:00
Clifford Clark, Chrystal Berry, Walter Demczuk Campylobacter transducer-like proteins (Tlps), also known as methyl-accepting chemotaxis proteins (MCPs), are associated with virulence as well as niche and host adaptation. While functional attributes of these proteins are being elucidated, little has been published regarding their sequence diversity or chromosomal locations and context, although...
Fri, 03/22/2019 - 11:00
Shiga toxin-producing Escherichia coli (STEC) and Campylobacter jejuni are notable health hazards associated with the consumption of raw milk. These bacteria may colonize the intestines of asymptomatic cattle and enter bulk tank milk via fecal contamination during milking. We studied the frequency of STEC O157:H7 and C. jejuni contamination in tank milk (n = 785) and the in-line milk filters of...
Fri, 03/22/2019 - 01:18
Sandip Shrestha, Basanta R. Wagle, Abhinav Upadhyay, Komala Arsi, Indu Upadhyaya, Dan J. Donoghue, Annie M. Donoghue
Sun, 03/17/2019 - 01:12
Available online 16 March 2019 Author(s): Roberta T. Melo, Ana Laura Grazziotin, Edson C.V. Júnior, Renata R. Prado, Eliane P. Mendonça, Guilherme P. Monteiro, Phelipe A.B.M. Peres, Daise A. RossiAbstractCampylobacter jejuni is the most common pathogen associated with foodborne diseases. Persistent presence of this pathogen contaminating the environment in slaughterhouses and chicken products...
Tue, 03/12/2019 - 10:19
Abstract Fresh fruit is indispensable as a part of a daily diet; however, it commonly harbors large and diverse bacteria populations, including some important human pathogens. To determine the microbial composition on the surface of a strawberry, and structural changes of microbial composition from the field and market, 16SrDNA sequencing of 32 samples collected from production field (B) and...
Sun, 03/03/2019 - 01:14
Publication date: Available online 28 February 2019Source: LWTAuthor(s): Lin Lin, Yulei Gu, Yanhui Sun, Haiying CuiAbstractFor improving stability of liposomes, the chitosan and pectin modified chrysanthemum essential oil (CHEO) liposomes were prepared. The average particle size of CHEO liposome was 132.4 nm with polymer dispersity index (PDI) of 0.152, zeta potential of −37.6 mV. Results showed...
Fri, 03/01/2019 - 01:11
Available online 26 February 2019 Author(s): Nereus W. Gunther, Aisha Abdul-Wakeel, O. Joseph Scullen, Christopher SommersAbstractRecent outbreaks of Campylobacter mediated disease attributed to undercooked chicken livers have highlighted a continuing need for methods to reduce Campylobacter numbers in these types of food products. In this study, gamma irradiation is evaluated for its...
Fri, 03/01/2019 - 01:11
Available online 27 February 2019 Author(s): Wendy Katiyo, Henrietta L. de Kock, Ranil Coorey, Elna M. BuysAbstractChicken meat has been identified as one of the most important food vehicles of pathogens, particularly Salmonella spp. and Campylobacter spp. Consumer food safety knowledge and behaviour can substantially contribute to the prevention of foodborne illness. The main objective of this...
Wed, 02/20/2019 - 11:00
Campylobacter bacteria are major human enteropathogens. Campylobacter coli shows less genetic diversity than C. jejuni and clusters into three clades, of which clade 1 includes most human and farm animal isolates, while environmental C. coli isolates mainly belong to clades 2 and 3. Recently, we sequenced the whole genomes of eight C. coli clade 2 and 3 isolates cultivated from water, and here we...
Tue, 02/12/2019 - 19:00
Campylobacter jejuni secretes HtrA (high temperature requirement protein A), a serine protease that is involved in virulence. Here, we investigated the interaction of HtrA with the host protein occludin, a tight ...
Wed, 02/06/2019 - 11:00
Poultry are considered a major reservoir and source of human campylobacteriosis, but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterized Campylobacter jejuni from western jackdaws (n = 91, 43%), mallard ducks (n = 82, 76%), and pheasants (n = 9, 9%). Most of the western jackdaw and mallard duck C. jejuni...
Wed, 02/06/2019 - 01:08
Publication date: August 2018Source: Microbial Risk Analysis, Volume 9Author(s): Mary E. Schoen, Michael A. Jahne, Jay GarlandAbstractWe assessed the annual probability of infection resulting from non-potable exposures to distributed greywater and domestic wastewater treated by an aerobic membrane bioreactor (MBR) followed by chlorination. A probabilistic quantitative microbial risk assessment...
Thu, 01/31/2019 - 01:13
Sun Ae Kim, Min Ji Jang, Seo Young Kim, Yichao Yang, Hilary O. Pavlidis, Steven C. Ricke
Thu, 01/31/2019 - 01:11
Quorum sensing is a widespread form of cell-to-cell communication, which is based on the production of signaling molecules known as autoinducers (AIs). The first group contains highly species-specific N-acyl homoserine lactones (N-AHLs), generally known as AI-1, which are produced by AHL synthase. The second group, possessing the characteristic structure of a furanone ring, are known as AI-2. The...
Mon, 01/28/2019 - 15:58
Campylobacter jejuni and Campylobacter coli are the most common cause of bacterial gastroenteritis in the world. Ganglioside mimicry by C. jejuni lipooligosaccharide (LOS) is the triggering factor of Guillain-Barré syndrome (GBS), an acute polyneuropathy. Sialyltransferases from the glycosyltransferase (GT) family 42 are essential for the expression of ganglioside mimics in C. jejuni....
Thu, 01/24/2019 - 11:00
Eimeria tenella can cause the disease coccidiosis in chickens. The direct and often detrimental impact of this parasite on chicken health, welfare, and productivity is well recognized; however, less is known about the secondary effects that infection may have on other gut pathogens. Campylobacter jejuni is the leading cause of human bacterial foodborne disease in many countries and has been...
Wed, 01/23/2019 - 01:08
Steven C. Ricke, Kristina M. Feye, W. Evan Chaney, Zhaohao Shi, Hilary Pavlidis, Yichao Yang
Fri, 01/18/2019 - 01:11
Available online 16 January 2019 Author(s): I. Clemente, S. Condón-Abanto, S. Pedrós-Garrido, P. Whyte, J.G. LyngAbstractThis work evaluated the bactericidal effects of a number of essential oils (EOs), organic acids and pulsed electric fields (PEF) against a range of Campylobacter jejuni strains. C. jejuni (1146DF) was found to be the most PEF resistant strain, and was chosen to evaluate the...

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