Research Publications (Food Safety)

Listeria monocytogenes is a concerning foodborne pathogen incriminated in soft cheese and meat-related outbreaks, highlighting the significance of applying alternative techniques to control its growth in food. In the current study, eco-friendly zinc oxide nanoparticles (ZnO-NPs) were synthesized using Rosmarinus officinalis, Punica granatum, and Origanum marjoram extracts individually.

Edible grasshopper, Ruspolia ruspolia, has nutritional and cherished cultural and economic importance to people from diverse cultures, particularly in over 20 African countries. It is consumed at home or commercially traded as sautéed, deep-fried, or boiled products. However, there is limited information on the hygiene practices of the vendors and the implications on the microbial safety of the final product.

This study evaluated the effects of bioactive feed ingredients on fecal microbiome of weaned pigs subjected to F18 enterotoxigenic Escherichia coli (ETEC) challenge. Two blocks of weaned pigs [n = 72; Yorkshire/Landrace/Duroc; initial body weight (BW) = 6.62 ± 1.37 kg] were balanced by gender and litter and randomly allocated to one of the six treatments with 12 pens/block and 6 pigs/pen.

The sheep rumen microbiome contains a vast wealth of known and unknown members, whose metabolic processes impact nutrient acquisition as well as gut and overall health of the animal. Based on previous research from our group on investigating the impact of a change in diet on the composition of the rumen microbiome in lambs, further insights into the metabolic functions of novel bacterial species represented by three Operational Taxonomic Units (OTUs) were pursued.

This study evaluated the effects of a sulfate polysaccharide extracted from marine algae product on performance, intestinal lesion scores, and mRNA abundance of various tight junction proteins and immune response genes.

Several feed supplements have received interest for their potential to increase growth performance and health post weaning, including alternatives to high levels of zinc oxide (ZnO) such as short- and medium-chain fatty acids (SCFA, MCFA), and phytogenics. The objective of this study is to observe the growth performance and intestinal health effects from the supplementation of SCFA with and without the addition of MCFA and phytogenics.

The activation of the immune system reduces the nutrient availability for growth and increases the variability among pigs. Functional amino acids (AA) supplementation can reduce the negative effects of immune activation. Additionally, providing daily tailored diets using individual precision feeding (IPF) was shown to account for individual variability.

Abstract Aims Our aim is to characterize through whole‐genome sequencing (WGS) the antimicrobial resistance (AMR) and heavy metal tolerance (HMT) genes content, plasmid presence, virulence potential and genomic diversity of the rare non‐typhoid Salmonella enterica serovar Orion (S. Orion) from 19 countries of the African, American, Eastern Mediterranean, European, Southeastern Asia and Western Pacific regions. Methods and Results Totally 324 S.

The study compared the effects of the flow regimes (laminar, transitional, and turbulent), treatment time, and the volume of water and plasma‐activated water bubbles circulation on the inactivation of the mixed‐species biofilms of Salmonella Typhimurium and Aeromonas australiensis grown on the inner surfaces of the polyvinyl chloride pipes. Abstract Biofilm formation in broiler drinking water systems is a public health concern.

Microbiology Resource Announcements, Ahead of Print.

Microbiology Resource Announcements, Ahead of Print.

Microbiology Resource Announcements, Ahead of Print.

Validation of traditional crust pepperoni pizza baking process. Abstract The objective of this study was to validate a simulated commercial baking process for traditional crust pepperoni pizza to inactivate Salmonella when contamination was introduced through inoculated flour and pepperoni slices.

Background

The pet industry is expanding worldwide, particularly raw meat-based diets (RMBDs). There are concerns regarding the safety of RMBDs, especially their potential to spread clinically relevant antibiotic-resistant bacteria or zoonotic pathogens.

Aim

IntroductionCryopreservation of semen can give full play to the reproductive advantages of male animals. However, in actual production, due to the poor frost resistance of sheep semen and the low conception rate, the promotion of sheep frozen semen is greatly hindered. Therefore, it is urgent to improve the frost resistance of semen to improve the quality of frozen semen.

Background Emergence and dissemination of antibiotic resistance is one of the major risks associated with the rampant usage of antibiotics in food-producing animals including aquaculture.

Background For pathogen detection in food, there are occasions where samples cannot be processed immediately after selective enrichment or need to be reexamined days or weeks later for confirmation or retest. Objective This study aimed to investigate the effect of different prolonged period of storage of selective enrichments of food at 4 ± 2°C and room temperature (20–22°C) on the detection and isolation of Salmonella.

This study investigates if a mixture of ribwort, sorrel, and yarrow extracts can reduce the thermal resistance of Salmonella Enteritidis in ground chicken. The thermal death kinetics calculated by curve fitting to the log‐linear and Weibull models revealed that the herbal extract mixture may provide Salmonella‐free cooked chicken products.

Applied and Environmental Microbiology, Ahead of Print. The emergence of foodborne Salmonella strains carrying antimicrobial resistance (AMR) in mobile genetic elements (MGE) is a significant public health threat in a One Health context requiring continuous surveillance. Resistance to ciprofloxacin and cephalosporins is of particular concern.

by A. Q. M. Robiul Kawser, M. Nazmul Hoque, M. Shaminur Rahman, Tahsin Islam Sakif, Tracey J. Coffey, Tofazzal Islam The field of fish microbiome research has rapidly been advancing, primarily focusing on farmed or laboratory fish species rather than natural or marine fish populations.

In this study, we aimed to investigate the effect of Scutellaria baicalensis and Lonicerae Flos (SL) extract on the growth performance and intestinal health of yellow-feather broilers following a Clostridium perfringens challenge.

This study investigated the effects of fish protein hydrolysate derived from barramundi on growth performance, muscle composition, immune response, disease resistance, histology and gene expression in white shrimp (Penaeus vannamei). In vitro studies demonstrated FPH enhanced mRNA expressions of key immune-related genes and stimulated reactive oxygen species (ROS) production and phagocytic activity in shrimp hemocytes.

Staphylococcus aureus is one of the primary pathogenic agents found in cheeses produced with raw milk. Some strains of S. aureus are enterotoxigenic, possessing the ability to produce toxins responsible for staphylococcal food poisoning when present in contaminated foods. This study aimed to genotypically characterize, assess the antimicrobial resistance profile, and examine the enterotoxigenic potential of strains of S.

Microviridae is a family of phages with circular ssDNA genomes and they are widely found in various environments and organisms. In this study, virome techniques were employed to explore potential members of Microviridae in a poultry slaughterhouse, leading to the identification of 98 novel and complete microvirus genomes.

Abstract Aims Contact with backyard poultry (i.e., privately‐owned, non‐commercial poultry) was first associated with a multistate outbreak of salmonellosis in 1955. In recent years, backyard poultry‐associated salmonellosis outbreaks have caused more illnesses in the United States than salmonellosis outbreaks linked to any other type of animal. Here, we describe the epidemiology of outbreaks from 2015–2022 to inform prevention efforts.