Research Publications (Food Safety)

Letters in Applied Microbiology, Accepted Article. The present study evaluated the antibacterial and antibiofilm activity of MHIR in combination with amoxicillin and clindamycin against biofilm-forming MRSA isolated from milk samples of mastitic cows. Microdilution susceptibility testing and microtiter plate assays were used to evaluate the in-vitro efficacy of MHIR and antibiotic combinations against MRSA (n=12).

Letters in Applied Microbiology, Accepted Article. Bovine by-products, such as liver, could be an underestimated source of Campylobacter jejuni. Therefore, our aims were to evaluate the occurrence of C.

Letters in Applied Microbiology, Accepted Article. One of the main drawbacks of chlorine disinfectants is the emergence of chlorine adapted (CA) or resistant microbial cells. This research aimed to investigate the effect of chlorine adaptation on resistance of Salmonella enterica upon atmospheric cold plasma (ACP) application at different voltages (6, 8 and 11 kV) and times (5, 10 and 15 min).

Letters in Applied Microbiology, Accepted Article. Fresh cucumbers have been recognized as a vehicle in foodborne disease outbreaks since several multistate outbreaks of salmonellosis linked to fresh cucumbers occurred in the United States. Little is known about how microbial cell surface characteristics that are known to affect adhesion can influence bacterial cross-contamination and transfer. This study investigated the role of S.

Letters in Applied Microbiology, Accepted Article. Food contamination with aflatoxin is one of the most critical concerns of health professionals. One of the best ways to reduce aflatoxin content in food is probiotics. Therefore, this study was performed to isolate Bifidobacterium from the chick's intestine; evaluate its probiotic activities and its application with Polyvinylpyrrolidone (PVP) to reduce aflatoxin B1 (AFB1) in the medium were investigated.

Letters in Applied Microbiology, Accepted Article. Numerous studies have attempted to restore the function of the tumour suppressor p53 as an anticancer strategy through gene delivery. However, most studies employed non-bacterial vectors to deliver p53.

Letters in Applied Microbiology, Accepted Article. Staphylococcus aureus is one of the main etiological agents causing foodborne diseases. Some strains produce enterotoxins responsible for food poisoning. In addition, they can form biofilms on several surfaces such as plastics, glass, stainless steel making it difficult to eliminate them.

Letters in Applied Microbiology, Accepted Article. Salmonella is a crucial foodborne pathogen causing food poisoning, leading to severe public health events. Here, we developed a technique by integrating recombinase polymerase amplification with CRISPR-LbCas12a and employing two targets with engineered crRNA for detection of Salmonella (RPA-LbCas12a-TTECDS).

Letters in Applied Microbiology, Accepted Article. The purpose of this study was to assess the health of stony coral Porites sp. based on the presence of bacterial pathogens, specifically Vibrio coralliilyticus, in the Gulf of Aqaba, and to assess the impact of anthropogenic activities on Porites sp. Porites sp. specimens were collected from the Marine Science Station (MSS) and a public beach (Yamanyeh) in Jordan.

Letters in Applied Microbiology, Accepted Article. Salmonella Typhi has been an important human restricted pathogen from time immemorial, and unfortunately, the indiscriminate use of antibiotics has induced the emergence of Multidrug-resistance in S. Typhi. Bacteriophage therapy may be a possible alternative in countering antimicrobial resistance.

Letters in Applied Microbiology, Accepted Article. The Curtobacterium genus is a member of the family Microbacteriaceae, and Curtobacterium species are recognized as plant pathogens. The aim of this study was to investigate a dubious result of species identification for an infection located on a catheter tip of a patient with Covid-19.

Letters in Applied Microbiology, Accepted Article. In order to develop a mechanistic bacterial dose-response model, based on the concept of Key Events Dose-Response Framework (KEDRF), this study aimed to investigate the invasion of intestinal model cells (Caco-2) by Salmonella Typhimurium and Listeria monocytogenes and described the behavior of both pathogens as a mathematical model using Bayesian inference.

Letters in Applied Microbiology, Accepted Article. This study was conducted to evaluate the performance of a screening protocol to detect and isolate mcr-positive E. coli and Salmonella spp. from animal caecal content and meat samples. We used a multicentre approach involving twelve laboratories from nine European countries.

Letters in Applied Microbiology, Accepted Article. CRISPR-Cas system contributes adaptive immunity to protect the bacterial and archaeal genome against invading mobile genetic elements. In this study, an attempt was made to characterize the CRISPR-Cas system in S. coagulans, the second most prevalent coagulase positive staphylococci causing skin infections in dogs.

Letters in Applied Microbiology, Accepted Article. Vibrio parahaemolyticus (V. parahaemolyticus) is a common marine foodborne pathogen that causes gastroenteritis. With the long-term use of antibiotics, many bacteria become resistant strains, therefore, developing antibiotic-free antimicrobial strategies is urgent.

Letters in Applied Microbiology, Accepted Article. Noroviruses cause acute gastroenteritis with symptoms of diarrhea and vomiting, and their high infectivity allows outbreaks to readily occur. Quickly identifying and isolating potential contaminants is an effective method to prevent the spread of outbreaks.

Letters in Applied Microbiology, Accepted Article. Antimicrobial resistance has silently turned into one of the biggest threats to global health, marking the fall of the Golden age of antibiotics. In the search for antibiotic replacement or enhancement, plant-derived natural compounds have attracted lots of interest. Even though firmly believed, the low resistance tendency of pathogenic bacteria against plant extracts has been scarcely demonstrated.

Letters in Applied Microbiology, Accepted Article. This is the first report of acute deaths in five European brown hares (Lepus europaeus) attributed to mucoid and necrotizing typhlocolitis caused by genetically different Cronobacter (C.) turicensis strains in northeastern Austria.

Letters in Applied Microbiology, Accepted Article. In this work, the antibacterial activity and mechanism of chloroform fraction obtained from aqueous extract of mugwort leaves against Staphylococcus aureus (S. aureus) were investigated. The extract showed obvious antibacterial activity against S.

Letters in Applied Microbiology, Accepted Article. Listeria monocytogenes is a typical foodborne pathogen that causes hard-to-treat bacterial infections, mainly due to its ability to form biofilm and enter into a viable but non-culturable state (VBNC). In this study, we investigated the removal effects of four antimicrobial agents on L.

Letters in Applied Microbiology, Accepted Article. We evaluated combined effects of vacuumed hydrogen peroxide vapor (VHPV) and vacuum-sealed dry heat (vacuum heat, VH) to inactivate foodborne pathogens (Salmonella Typhimurium, Escherichia coli O157:H7, and Listeria monocytogenes) on alfalfa seeds.

Letters in Applied Microbiology, Accepted Article. Enterohemorrhagic Escherichia coli O157:H7 (EHEC) causes foodborne outbreaks worldwide and the bacteria form antimicrobial-tolerant biofilm. We investigated the abilities of Thymus daenensis and Satureja hortensis essential oils (EOs) to inhibit bacterial growth, biofilm formation and quorum sensing (QS) by E. coli O157:H7.

Letters in Applied Microbiology, Accepted Article. The genetic fusion of cytolysin A (ClyA) to heterologous antigen expressed in live Salmonella vector demonstrated efficient translocation into periplasmic space and extracellular medium. Accumulating evidence has shown that clyA-mediated antigen delivery improved growth fitness and enhanced immunogenicity of live vector vaccine, but the factors influencing this protein exportation has not been investigated.

Letters in Applied Microbiology, Accepted Article. Methicillin-resistant Staphylococcus aureus (MRSA) is a multidrug-resistant opportunistic pathogen with a great ability to form biofilms. Herein, the antimicrobial potential of Thymbra capitata essential oil (EO) against MRSA biofilms was investigated. The determination of the minimum inhibitory concentration (MIC) and the minimum lethal concentration (MLC) of the T.

Letters in Applied Microbiology, Accepted Article. Artocarpin-rich extract (ARE) was prepared using a green technology and standardized to contain 49.6% w/w artocarpin, while lawsone methyl ether was prepared using a green semi-synthesis. ARE, LME and ampicillin exhibited weak anti-MRSA activity with the MICs of 31.2-62.5 µg/mL.