Research Publications (Food Safety)

Bradd J. Haley, James Pettengill, Sasha Gorham, Andrea Ottesen, Jeffrey S. Karns, Jo Ann S. Van Kessel

Guillermo Cebrián, Pilar Mañas, Santiago Condón

Cédric Woudstra, Caroline Le Maréchal, Rozenn Souillard, Marie-Hélène Bayon-Auboyer, Isabelle Mermoud, Denise Desoutter, Patrick Fach

Yi Chen, Emma Allard, Anna Wooten, Minji Hur, Ishani Sheth, Anna Laasri, Thomas S. Hammack, Dumitru Macarisin

Xiaona Shen, Jingyun Zhang, Jialiang Xu, Pengcheng Du, Bo Pang, Jie Li, Biao Kan

Chun-Xue Zhou, Hany M. Elsheikha, Dong-Hui Zhou, Qing Liu, Xing-Quan Zhu, Xun Suo

Hui Huang, Yawei Sun, Li Yuan, Yushan Pan, Yanlin Gao, Caihui Ma, Gongzheng Hu

Diogo T. da Cunha, Ana L. de Freitas Saccol, Eduardo C. Tondo, Ana B. A. de Oliveira, Veronica C. Ginani, Carolina V. Araújo, Thalita A. S. Lima, Angela K. F. de Castro, Elke Stedefeldt

Wonhee Cha, Rebekah Mosci, Samantha L. Wengert, Pallavi Singh, Duane W. Newton, Hossein Salimnia, Paul Lephart, Walid Khalife, Linda S. Mansfield, James T. Rudrik, Shannon D. Manning Campylobacter jejuni is a zoonotic pathogen and the most common bacterial cause of human gastroenteritis worldwide. With the increase of antibiotic resistance to fluoroquinolones and macrolides, the drugs of choice for treatment, C. jejuni was recently classified as a serious antimicrobial resistant threat.

Daniel Ryan, Urmesh K. Ojha, Sangeeta Jaiswal, Chandrashekhar Padhi, Mrutyunjay Suar The Gram-negative, enteropathogen Salmonella enterica serovar Typhimurium (S. Typhimurium) is exposed to various stress conditions during pathogenesis, of which acid stress serves as a major defense mechanism in the host. Such environments are encountered in the stomach and Salmonella containing vacuole of phagocytic and non-phagocytic cells.

Anke Stüken, Pilar Riobó, José Franco, Kjetill Sigurd Jakobsen, Laure Guillou and Rosa Isabel Figueroa Dinoflagellates are microscopic aquatic eukaryotes with huge genomes and an unusual cell regulation. For example, most genes are present in numerous copies and all copies seem to be obligatorily transcribed. The consequence of the gene copy number (CPN) for final protein synthesis is, however, not clear. One such gene is sxtA, the starting gene of paralytic shellfish toxin (PST) synthesis.

Lisa Barco, Federica Barrucci, Enzo Cortini, Elena Ramon, John Elmerdahl Olsen, Ida Luzzi, Antonia Anna Lettini and Antonia Ricci The current picture of human salmonellosis shows Salmonella Typhimurium and S. 4,[5],12:i:- as the most common serovars in Italy. The aims of this study were to investigate the genetic relationship between these serovars, as well as to test the possibility of inferring sources of human salmonellosis due to S. Typhimurium and S.

Feng Xu, Saba Ilyas, Jeffrey A. Hall, Stephen H. Jones, Vaughn S. Cooper and Cheryl A. Whistler Gastric infections caused by the environmentally transmitted pathogen, Vibrio parahaemolyticus, have increased over the last two decades, including in many parts of the United States (US). However, until recently, infections linked to shellfish from the cool northeastern US waters were rare. Cases have risen in the Northeast, consistent with changes in local V.

Yufei Wang Bacterial small non-coding RNAs (sRNAs) are gene expression modulators respond to environmental changes, stressful conditions, and pathogenesis. In this study, by using a combined bioinformatic and experimental approach, eight novel sRNA genes were identified in intracellular pathogen Brucella melitensis. BSR0602, one sRNA that was highly induced in stationary phase, was further examined and found to modulate the intracellular survival of B. melitensis.

Sonia Boughattas

A commentary on
Detection of Toxoplasma gondii in raw caprine, ovine, buffalo, bovine, and camel milk using cell cultivation, cat bioassay, capture ELISA, and PCR methods in Iran

Dehkordi, F. S., Borujeni, M. R., Rahimi, E., and Abdizadeh, R. (2013). Foodborne Pathog. Dis. 10, 120–125. doi: 10.1089/fpd.2012.1311

Keila Martin-Betancor, Ismael Rodea-Palomares, Angeles Muñoz Martin, Francisco Leganes and Francisca FERNÁNDEZ-PIÑAS A self-luminescent bioreporter strain of the unicellular cyanobacterium Synechococcus sp. PCC 7942 was constructed by fusing the promoter region of the smt locus (encoding the transcriptional repressor SmtB and the metallothionein SmtA) to luxCDABE from Photorhabdus luminescens; the sensor smtB gene controlling the expression of smtA was cloned in the same vector.

Rongzhi Wang, Yangfang Zhong, Xiaosong Gu, Jun Yuan, Abdullah Farhan ul haque Saeed and Shihua Wang Vibrio parahaemolyticus, a Gram-negative motile bacterium that inhabits marine and estuarine environments throughout the world, is a major food-borne pathogen that causes life-threatening diseases in humans after the consumption of raw or undercooked seafood. The global occurrence of V.

Ying Yu, Lihua Fang, Yan Zhang, Hongxia Sheng and Weihuan Fang Type VI secretion system (T6SS) is a macromolecular transenvelope machine encoded within the genomes of several proteobacteria species. Vibrio parahaemolyticus contains two putative T6SS systems, VpT6SS1 and VpT6SS2, both contributing to adherence to Caco-2 and/or HeLa cells. However, it remains unknown if these systems are involved in cellular responses.

Juan-Carlos Gutierrez, Francisco Amaro and Ana Martin-Gonzalez This review analyzes the advantages and disadvantages of using eukaryotic microorganisms to design whole-cell biosensors (WCBs) for monitoring environmental heavy metal pollution in soil or aquatic habitats. Basic considerations for designing a eukaryotic WCB are also shown.

Neha Rajpara, Braj Mohan Ram Narayan Singh Kutar, Ritam Sinha, Dhrubajyoti Nag, Hemanta Koley, Thandavarayan Ramamurthy and Ashima Kushwaha Bhardwaj The isolates of Vibrio cholerae and Providencia vermicola obtained from a diarrheal patient were investigated for genetic elements governing their drug resistance phenotypes. Out of 14 antibiotics tested, V. cholerae Vc IDH02365 isolate showed resistance to nine antibiotics, while P.

Rauna Riva, Timo K. Korhonen and Seppo Meri The virulence factor PgtE is an outer membrane protease (omptin) of the zoonotic pathogen Salmonella enterica that causes diseases ranging from gastroenteritis to severe enteric fever. It is surface exposed in bacteria that have a short-chain, i.e., rough LPS, as observed e.g., in bacteria residing inside macrophages or just emerging from them.

Brian William Brunelle, Bradley L. Bearson and Shawn M.D. Bearson Salmonella enterica serovar Typhimurium is one of the most common serovars isolated from humans and livestock, and over 35% of these isolates are resistant to three or more antibiotics.

Changyong Cheng, Yongchun Yang, Zhimei Dong, Xiaowen Wang, Chun Fang, Menghua Yang, Jing Sun, Liya Xiao, Weihuan Fang and Houhui Song Listeria monocytogenes, a food-borne pathogen, has the capacity to maintain intracellular pH (pHi) homeostasis in acidic environments, but the underlying mechanisms remain elusive.

Yael Kotton, Michal Gordon, Vered Chalifa-Caspi and Naiel Bisharat In 1996 a common-source outbreak of severe soft tissue and bloodstream infections erupted among Israeli fish farmers and fish consumers due to changes in fish marketing policies. The causative pathogen was a new strain of Vibrio vulnificus, named biotype 3, which displayed a unique biochemical and genotypic profile.

Ian F. Connerton and Steven P.T. Hooton Campylobacter jejuni is a worldwide cause of human diarrhoeal disease. Clustered Repetitively Interspaced Palindromic Repeats (CRISPRs) and associated proteins allow Bacteria and Archaea to evade bacteriophage and plasmid infection. Type II CRISPR systems are found in association with combinations of genes encoding the CRISPR-associated Cas1, Cas2, Cas4 or Csn2, and Cas9 proteins. C.