Research Publications (Food Safety)

Shikonin has been used in food production because of its potent bacteriostatic activity and colorability, but it is limited to be widely used because of its poor water solubility. The aim of this study was to prepare shikonin @ chitosan nanoemulsion (NS) to improve the solubility of shikonin and evaluate its antibacterial activity against Staphylococcus aureus (S. aureus) and the effect on storage quality of a pork food model.

This study was designed to isolate and characterize novel lytic phages specific to Staphylococcus aureus (S. aureus) from wastewater samples, determine their antimicrobial and antibiofilm activities, and investigate the biocontrol efficiency of phages on raw beef contaminated with S. aureus. For this purpose, a total of 50 wastewaters from slaughterhouses were used as material, and phages were isolated with the double-layer agar method.

Staphylococcus aureus is a major pathogen capable of causing foodborne diseases (FBDs) in fresh meat products and ready-to-eat (RTE) sandwiches. Therefore, investigating antimicrobial therapies that are safe and efficacious will benefit food safety.

The peptide MOp3 from Moringa oleifera seeds has been reported to have good antimicrobial activity against Staphylococcus aureus, but its specific antimicrobial effect is still unclear. Therefore, the present study aimed to investigate the antibacterial properties of MOp3 against S.

Staphylococcus aureus (S. aureus) is widely distributed in the environment, which can easily lead to food contamination. Thus, it is essential to develop rapid and on-site detection of S. aureus. In the present study, an intuitive colorimetric method based on phage display technology (PDT), immunomagnetic separation (IMS), and polymerase spiral reaction (PSR) was successfully established for S. aureus detection.

Due to the rich source of compounds, milk and dairy products are an excellent environment for the growth of many groups of microorganisms, including Staphylococcus aureus. The presence of pathogens in raw materials can threaten the dairy industry around the world.

This work investigated the antibacterial activity and mechanism of canthin-6-one (CAN) on Staphylococcus aureus (S. aureus). CAN could inhibit the growth of S. aureus with minimum inhibitory concentration (MIC) of 16 μg/mL, exhibiting weak trends in resistance, good biosafety and acceptable stability. Furthermore, 5.30 log-units of S. aureus in beef soup were reduced after treated by 32 μg/mL of CAN at 37 oC for 3 days.

At present, the main challenge for Staphylococcus aureus (S. aureus) in food safety is still a lack of rapid, convenient detection methods. This study developed a combination of recombinase polymerase amplification (RPA) and the CRISPR-Cas12a technology (known as the RPA-CRISPR-Cas12a assay) for the detection of S. aureus in food.

Rapid and accurate screening of foodborne pathogens in contaminated food is with great significance for food safety. AuNPs-based colorimetric assay is an attractive strategy in the field of food safety inspection, but is limited by tedious probe preparation, low color rendering resolution, and narrow color gamut.

Staphylococcus aureus (S. aureus) is a pathogenic bacterium that can cause serious contamination in meat. Herein, a novel fluorescence biosensor was designed for the rapid detection of S. aureus based on mesoporous silica-modified upconversion nanoparticles (UCNPs-mSiO2).

BCp12, a novel antimicrobial agent derived from buffalo milk, poses superior antimicrobial activity against Staphylococcus aureus. In the present study, the effect of BCp12 on the cell wall synthesis and molecular pathways of Staphylococcus aureus was studied by the CLSM, SEM and proteomics analyses to facilitate the development of this novel antimicrobial peptide.

This study aims to elucidate the inhibitory activity of nisin alone or in combination with gilaburu (Viburnum opulus L.) against the foodborne Staphylococcus aureus. The effect of nisin and gilaburu on the growth of S. aureus was investigated under different pH (5–7) and salt concentrations (NaCl, 0.5–4.5% w/v) at 30 °C.

This study aimed to develop innovative micro-nano fibers loaded with lemon peel essential oil for food packaging via centrifugal spinning. Lemon peel essential oil (LPO) was extracted from dried lemon peel by hydrodistillation. The major one of the 16 components of the extract detected by GS-MS was limonene (60.4%). Gelatin fibrous mats loaded with three different concentrations of LPO were fabricated with centrifugal spinning and then cross-linked.

The safety risks of antimicrobial peptide (AMP) resistance have never been properly understood. Herein, we comprehensively investigated the evolution of resistance to AMPs in Staphylococcus aureus using piscidin1 (PIS-1) and piscidin3 (PIS-3) as archetypes, in which they were both combined with cell membranes via a common structural motif but vary in permeabilizing actions.

The inhibitory mechanism of CUM on Staphylococcus aureus exotoxins and invasive enzyme, and the effect of CUM on sauced beef were investigated. Treatment with sub-minimum inhibitory concentrations of CUM observed very little decrease in cell density after 24 h. Additionally, CUM reduced hemolytic activity by inhibiting the formation of heptamer, and inhibited the content of enterotoxin in culture medium and sauced beef.

Food-borne Staphylococcus aureus has shown multidrug resistance. According to our previous studies, most S. aureus isolated from retail foods in China exhibited resistance to a variety of antimicrobials. Further Genome Wide Association Study (GWAS) revealed that a novel gene cluster, containing erythromycin-resistant genes, mef(D)-msr(F), existed in 19 isolates.

Author(s): Feng Zhang, Shi Wu, Jiahui Huang, Jingsha Dai, Jumei Zhang, Runshi Yang, Liang Xue, Miao Zhao, Juan Wang, Yu Ding, Moutong Chen, Qingping Wu Food-borne Staphylococcus aureus has shown multidrug resistance. According to our previous studies, most S. aureus isolated from retail foods in China exhibited resistance to a variety of antimicrobials.

Author(s): Kateřina Dorotíková, Josef Kameník, Kateřina Bogdanovičová, Simona Křepelová, Jan Strejček, Danka Haruštiaková

Author(s): Jiwon Oh, Hoikyung Kim, Larry R. Beuchat, Jee-Hoon Ryu

Author(s): Daniela Bencardino, Giulia Amagliani, Giorgio Brandi

Author(s): G.K. Sivaraman, K.H. Muneeb, S. Sudha, Bibek Shome, Jennifer Cole, Mark Holmes

Author(s): Huixue Cai, Xiaomeng Kou, Hua Ji, Xin Wang, Haixia Wang, Yan Zhang, Shili Lu, Baokun Li, Juan Dong, Qingling Wang, Jing Zhou, Die Hu

Author(s): Wen-Li Seow, Nor Ainy Mahyudin, Syafinaz Amin-Nordin, Son Radu, Noor Azira Abdul-Mutalib

Author(s): Xiaonan Zhao, Xiaomeng Yuan, Ming Hu, Yin Zhang, Lulu Li, Qing Zhang, Xuexia Yuan, Wenbo Wang, Yuqing Liu

Author(s): Mengyuan Qi, Ruoqing Zhao, Qingyan Liu, Haiyang Yan, Yan Zhang, Shiyi Wang, Yuan Yuan