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Research Publications (Food Safety)

This page tracks research articles published in national and international peer-reviewed journals. Recent articles are available ahead of print and searchable by Journal, Article Title, and Category. Research publications are tracked across six categories: Bacterial Pathogens, Chemical Contaminants, Natural Toxins, Parasites, Produce Safety, and Viruses. Articles produced by USDA Grant Funding Agencies (requires login) and FDA Grant Funding Agencies (requires login) are also tracked in Scopus.

Displaying 1 - 25 of 546

  1. Fate of salmonella and shiga-toxin producing Escherichia coli on wheat grain during tempering

    • Sun, 11/27/2022 - 04:00
    • Food Microbiology
    • Outbreaks of Salmonella and Shiga-toxin producing Escherichia coli (STEC) linked to wheat flour led to increased interest in characterizing the fate of Salmonella and STEC on wheat during processing. Tempering is the stage of wheat processing where water is added to toughen the bran prior to milling, which has the potential to influence pathogen behavior on the kernels.

      • Bacterial pathogens
      • Salmonella
  2. Serotype-dependent adhesion of Shiga toxin-producing Escherichia coli to bovine milk fat globule membrane proteins

    • Thu, 11/24/2022 - 04:00
    • Frontiers in Microbiology
    • Shiga toxin-producing Escherichia coli (STEC) are food-borne pathogens that can cause severe symptoms for humans. Raw milk products are often incriminated as vehicule for human STEC infection. However, raw milk naturally contains molecules, such as the milk fat globule membrane and associated proteins, that could inhibit pathogen adhesion by acting as mimetic ligands.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  3. Comparative genomic and phenotypic analyses of the virulence potential in Shiga toxin-producing Escherichia coli O121:H7 and O121:H10

    • Thu, 11/24/2022 - 04:00
    • Frontiers in Cellular and Infection Microbiology
    • Shiga toxin-producing Escherichia coli (STEC) O121 is among the top six non-O157 serogroups that are most frequently associated with severe disease in humans. While O121:H19 is predominant, other O121 serotypes have been frequently isolated from environmental samples, but their virulence repertoire is poorly characterized.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  4. Shiga toxin (stx) encoding genes in sheep and goats reared in Trinidad and Tobago

    • Wed, 11/16/2022 - 04:00
    • PLOS ONE
    • by Anil K. Persad, Gireesh Rajashekara, Jeffrey T. LeJeune Shiga toxin-producing Escherichia coli (STEC) is estimated to cause over two million cases of human disease annually. Trinidad and Tobago is one of the largest livestock producer and consumer of sheep and goat meat in the Caribbean, however, the potential role of these animals in the epidemiology of STEC infections has not been previously described.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  5. Effect of an Eco-Friendly Cuminaldehyde Guanylhydrazone Disinfectant on Shiga Toxin Production and Global Transcription of Escherichia coli

    • Thu, 11/03/2022 - 04:00
    • Toxins
    • Antimicrobials have been important medicines used to treat various infections. However, some antibiotics increase the expression of Shiga toxin (Stx). Also, the pervasive use of persistent antibiotics has led to ecotoxicity and antibiotic resistance. In this study, a newly developed broad-spectrum and reversible antibiotic (guanylhydrazone disinfectant) was evaluated for its antibiotic activity and effects on Stx production and global transcription of bacteria.

      • Bacterial pathogens
      • Escherichia coli O157:H7
  6. Effects of acidic water tempering and heat treatment on the Shiga toxin‐producing Escherichia coli (O121 and O26) load of wheat during tempering and its impact on wheat flour quality

    • Sat, 10/29/2022 - 04:00
    • Journal of Food Processing and Preservation
    • Journal of Food Processing and Preservation, Volume 46, Issue 10, October 2022. The study investigated the effects of acidic water (sodium bisulfate, SBS/lactic acid, LA) tempering (alone) and in combination with heat treatment on the Shiga toxin-producing Escherichia coli (STEC) O121 and O26 load of wheat and its impact on wheat flour quality.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  7. Biofilm formation, antibiotic resistance and genotyping of Shiga toxin-producing Escherichia coli isolated from retail chicken meats

    • Thu, 10/20/2022 - 04:00
    • British Poultry Science
    • 1. The Shiga toxin-producing Escherichia coli (STEC) is a hazardous zoonotic agent for chicken meat consumers. This study determined the serogroups and evaluated the virulence genes, antibiotic resistance, biofilm-forming profiles and genetic relationships of STEC isolates in chicken meat.

      • Bacterial pathogens
      • Escherichia coli O157:H7
  8. DNA concentration by solid phase reversible immobilization improves its yield and purity, and detection time of E. coli O157:H7 in foods by high resolution melt curve qPCR

    • Thu, 10/20/2022 - 04:00
    • Food Control
    • For molecular detection of foodborne pathogens to be effective, an efficient technique for bacterial DNA concentration is required. Solid phase reversible immobilization (SPRI) paramagnetic beads can concentrate DNA from foods containing low amounts of bacteria because of their highly specific DNA-binding capacity.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  9. Optimizing Escherichia coli O157:H7 inactivation in goat's milk by thermosonication

    • Wed, 10/19/2022 - 04:00
    • Journal of Food Process Engineering
    • Journal of Food Process Engineering, EarlyView. Escherichia coli O157:H7 (STEC) is one of the hazardous foodborne pathogens in milk. Although traditional preservation methods reduce contamination, they are time-consuming or cause physicochemical changes. Therefore, we optimize STEC inactivation in goat's milk by thermosonication, an alternative to traditional treatments.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  10. Validation of baking as a kill-step for controlling Shiga toxin-producing Escherichia coli during traditional crust pizza baking process

    • Thu, 10/06/2022 - 04:00
    • Frontiers in Microbiology
    • A study was conducted to validate a simulated traditional crust pepperoni pizza baking process to control Shiga toxin-producing Escherichia coli (STEC) and to determine the heat resistance characteristics of STEC in pizza dough. Pizza dough and pepperoni slices were inoculated with 7 strains STEC cocktail and baked at 500°F (260°C) for 12 min using a conventional kitchen oven followed by 15 min of ambient air cooling.

      • Bacterial pathogens
      • Escherichia coli O157:H7
  11. Transduction of stx2a mediated by phage (Φ11-3088) from Escherichia coli O104:H4 in vitro and in situ during sprouting of mung beans

    • Fri, 09/30/2022 - 04:00
    • International Journal of Food Microbiology
    • Escherichia coli O104:H4 strain 11-3088 encoding Stx2a is epidemiologically related to the foodborne outbreak associated with sprouts in Germany, 2011. Sprouting provides suitable conditions for bacterial growth and may lead to transduction of non-pathogenic strains of E. coli with Stx phages. Although transduction of E. coli by Stx phages in food has been documented, data on the phages from E. coli O104:H4 is limited.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  12. Prevalence and molecular characterization of shiga toxin-producing Escherichia coli in animal source foods and green leafy vegetables

    • Wed, 09/14/2022 - 04:00
    • Food Science and Technology International
    • Shiga toxin–producing Escherichia coli (STEC) has emerged as important enteric foodborne zoonotic pathogens of considerable public health significance worldwide. The aim of this study was to determine the prevalence of the top seven STEC serotypes and to identify these serotypes in samples of animal source foods and vegetables. A total of 294 samples including 84 meat samples, 135 milk and dairy product samples and 75 green leafy vegetable samples were tested.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
      • Produce Safety
      • Leafy Greens
  13. Exploring the nature of interaction between shiga toxin producing Escherichia coli (STEC) and free-living amoeba - Acanthamoeba sp

    • Sat, 09/10/2022 - 04:00
    • Frontiers in Cellular and Infection Microbiology
    • Free-living amoebae (FLA) are widely distributed protozoa in nature, known to cause severe eye infections and central nervous system disorders. There is growing attention to the potential role that these protozoa could act as reservoirs of pathogenic bacteria and, consequently, to the possibility that, the persistence and spread of the latter may be facilitated, by exploiting internalization into amoebae.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  14. Survival of Salmonella and Shiga toxin–producing Escherichia coli during tempering of wheat berries

    • Wed, 09/07/2022 - 04:00
    • Food Control
    • Wheat flour contaminated with Salmonella or Shiga toxin–producing Escherichia coli (STEC) has been linked to foodborne outbreaks and recalls. The survival of five strains of rifampicin-resistant Salmonella and STEC on wheat berries was evaluated during tempering (hydration). Wheat berries were inoculated with Salmonella (cocktail) or STEC (individual strains or cocktail) and dried at ambient conditions for 24 h.

      • Bacterial pathogens
      • Salmonella
  15. Genomic Analysis of Shiga Toxin-Producing E. coli O157 Cattle and Clinical Isolates from Alberta, Canada

    • Thu, 09/01/2022 - 04:00
    • Toxins
    • Shiga toxin (stx) is the principal virulence factor of the foodborne pathogen, Shiga toxin-producing Escherichia coli (STEC) O157:H7 and is associated with various lambdoid bacterio (phages). A comparative genomic analysis was performed on STEC O157 isolates from cattle (n = 125) and clinical (n = 127) samples to characterize virulence genes, stx-phage insertion sites and antimicrobial resistance genes that may segregate strains circulating in the same geographic region.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  16. Molecular characterization and phylogeny of Shiga toxin-producing Escherichia coli derived from cattle farm

    • Thu, 08/04/2022 - 04:00
    • Frontiers in Microbiology
    • Shiga toxin-producing Escherichia coli (STEC) is an important food-borne pathogen, which can cause diseases such as diarrhea, hemorrhagic enteritis, and hemolytic uremic syndrome in humans. Twelve STEC isolates were collected from beeves and feces of commercial animals in China between 2019 and 2020 for this study.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  17. Insertion Sequence (IS)-Excision Enhancer (IEE)-Mediated IS Excision from the lacZ Gene Restores the Lactose Utilization Defect of Shiga Toxin-Producing Escherichia coli O121:H19 Strains and Is Responsible for Their Delayed Lactose Utilization Phenotype

    • Tue, 08/02/2022 - 04:00
    • Applied and Environmental Microbiology
    • Applied and Environmental Microbiology, Ahead of Print. Lactose utilization is one of the general biochemical characteristics of Escherichia coli, and the lac operon is responsible for this phenotype, which can be detected on lactose-containing media, such as MacConkey agar, after 24 h of incubation. However, some Shiga toxin-producing E.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  18. Virulence and antimicrobial-resistance of shiga toxin-producing E. coli (STEC) Isolated from edible shellfish and its public health significance

    • Fri, 07/22/2022 - 04:00
    • Archives of Microbiology
    • Shiga toxin-producing E. coli (STEC) are an important cause of foodborne illness in humans with infections ranging from mild non-bloody diarrhea to bloody diarrhea (BD) and hemolytic uremic syndrome (HUS). This study aimed to investigate the distribution of STEC in shellfish from coastal shores of Lake Timsah in Ismailia Governorate, Egypt and its probable hazard to seafood consumers.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
      • Escherichia coli O157:H7
  19. Draft Genome Sequences of Multidrug-Resistant Shiga Toxin-Producing Escherichia coli O116:H25 Strains from Ready-to-Eat Foods Sold in Lagos, Nigeria

    • Thu, 07/14/2022 - 04:00
    • Microbiology Resource Announcements
    • Microbiology Resource Announcements, Ahead of Print. Draft genomes of multidrug-resistant Shiga toxin-producing Escherichia coli (STEC) strains IPK9(1) and IKS1(2), which were isolated from ready-to-eat foods (kokoro and shawarma) sold in Lagos, Nigeria, are reported. The genomes possessed genetic determinants for virulence and the antibiotic resistance gene for macrolide-associated resistance mdf(A). Ready-to-eat foods increase public health threats in Nigeria.

      • Bacterial pathogens
      • Escherichia coli O157:H7
  20. Genomic Diversity, Virulence Gene, and Prophage Arrays of Bovine and Human Shiga Toxigenic and Enteropathogenic Escherichia coli Strains Isolated in Hungary

    • Tue, 07/05/2022 - 04:00
    • Frontiers in Microbiology
    • Escherichia coli belonging to the enterohemorrhagic (EHEC), Shiga toxin-producing (STEC) and atypical enteropathogenic (aEPEC) pathotypes are significant foodborne zoonotic pathogens posing serious health risks, with healthy cattle as their main reservoir. A representative sampling of Hungarian cattle farms during 2017–2018 yielded a prevalence of 6.5 and 5.8% for STEC and aEPEC out of 309 samples.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  21. Microbiological quality of ready-to-eat salad products collected from retail and catering settings in England during 2020-21

    • Sat, 07/02/2022 - 04:00
    • Salad and other fresh produce were collected in England from retail and catering during 2020-21 and  were tested for Salmonella, Shiga-toxin producing Escherichia coli (STEC), Listeria , Bacillus cereus , and generic E. coli. Of the 604 samples collected , 57% were from retail and 43% from catering: 61% were either salad leaves or salad leaves mixed with other products. Equal numbers of samples were prepacked or loose and 50% were refrigerated at the time of sampling.

      • Bacterial pathogens
      • Bacillus cereus
      • Listeria monocytogenes
      • Salmonella
  22. Microbial Antagonism in Food-Enrichment Culture: Inhibition of Shiga Toxin-Producing Escherichia coli and Shigella Species

    • Thu, 06/23/2022 - 04:00
    • Frontiers in Microbiology
    • Bacterial pathogens, such as Shiga toxin-producing Escherichia coli (STEC) and Shigella spp., are important causes of foodborne illness internationally. Recovery of these organisms from foods is critical for food safety investigations to support attribution of illnesses to specific food commodities; however, isolation of bacterial cultures can be challenging. Methods for the isolation of STEC and Shigella spp.

      • Bacterial pathogens
      • Escherichia coli O157:H7
      • Shigella
  23. Draft Genome Sequences of Tetracycline-Resistant Shiga Toxin-Producing Escherichia coli Isolates from Food

    • Tue, 06/14/2022 - 04:00
    • Microbiology Resource Announcements
    • Microbiology Resource Announcements, Ahead of Print. Shiga toxin-producing Escherichia coli (STEC) is a foodborne pathogen transmitted from animal to humans through contaminated food. Here, we report the draft genome sequences of six STEC isolates (six serotypes) from food (cheese, coriander, and pea protein pellets) in different countries; these isolates were resistant to tetracycline, with MIC values ranging from <1.5 to 256 μg/mL.

      • Bacterial pathogens
      • Escherichia coli O157:H7
  24. Occurrence of multiple drug-resistant Shiga toxigenic Escherichia coli in raw milk samples collected from retail outlets in South India

    • Sat, 06/04/2022 - 04:00
    • Journal of Food Science and Technology
    • The prevalence of Shiga-toxigenic E. coli (STEC), harbouring multidrug-resistant genes in raw milk collected from household vendors and cooperative milk marketing societies in Ernakulam District, Kerala, India, has been investigated. A higher prevalence of STEC pathotypes, with 47.16% (20 out of 42 samples), was observed in raw milk. A total of 157 STEC isolates were identified, which included notorious pathoserotypes, E. coli O157 group (10.19%) and E.

      • Bacterial pathogens
      • Enterohemorrhagic Escherichia coli
  25. A bacteriological survey of fresh minced beef on sale at retail outlets in Scotland in 2019: three food-borne pathogens, hygiene process indicators and phenotypic antimicrobial resistance.

    • Fri, 06/03/2022 - 04:00
    • The health and economic burden of foodborne illness is high, with approximately 2.4 million cases occurring annually in the United Kingdom. A survey to understand the baseline microbial quality and prevalence of food-related hazards of fresh beef mince on retail sale could inform risk assessment, management and communication to ensure the safety of this commodity.

      • Bacterial pathogens
      • Campylobacter
      • Salmonella