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National Agricultural Library
U.S. Department of Agriculture
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  4. GENOMIC AND HORTICULURAL APPROACHES TO MANAGE BLACKLINE DISEASE IN WALNUTS

GENOMIC AND HORTICULURAL APPROACHES TO MANAGE BLACKLINE DISEASE IN WALNUTS

Objective

1) Screen transgenic walnut lines expressing shRNA for resistance to Cherry leaf roll virus (CLRV). 2) Identify recessive alleles that can confer resistance to CLRV in J. regia. 3) Clone two eIf4E genes identified in J. regia transcriptome and evaluate their interaction with VPg of CLRV. 4) Edit the eIF4E in walnuts using CRISPR system.

Investigators
Sudarshana M R
Institution
USDA - Agricultural Research Service
Start date
2019
End date
2020
Funding Source
Agricultural Research Service
Project number
2032-22000-016-54R
Accession number
436310
Commodities
Nuts, Seeds
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