Microarray Analysis of Gene Function and Host Interaction in Salmonella Typhimurium (Salarray)

<ol> <LI> The construction of a complete and definitive gene array for S.typhimurium based on its genomic sequence <LI> Creation of a microarray of a selected set of chicken genes involved in host response to Salmonella infections. <LI> Optimised procedures for the preparation of RNA, labeling and hybridisation for the Salmonella and chicken gene arrays<LI> Gene expression profiles for S.typhimurium growing under different nutrient and redox conditions <LI> Gene expression profiles for strains containing known mutations in genes affecting growth and virulence <LI> Gene expression profiles for S.typhimurium genes and host genes expressed during adhesion and invasion of host cells <LI> Gene expression profiles for S.typhimurium genes and host genes expressed in interactions between macrophages and S.typimurium